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Biogeosciences An interactive open-access journal of the European Geosciences Union
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BG | Volume 17, issue 10
Biogeosciences, 17, 2767–2789, 2020
© Author(s) 2020. This work is distributed under
the Creative Commons Attribution 4.0 License.

Special issue: Assessing environmental impacts of deep-sea mining...

Biogeosciences, 17, 2767–2789, 2020
© Author(s) 2020. This work is distributed under
the Creative Commons Attribution 4.0 License.

Research article 26 May 2020

Research article | 26 May 2020

Assessing the temporal scale of deep-sea mining impacts on sediment biogeochemistry

Assessing the temporal scale of deep-sea mining impacts on sediment biogeochemistry
Laura Haffert1, Matthias Haeckel1, Henko de Stigter2, and Felix Janssen3,4 Laura Haffert et al.
  • 1GEOMAR Helmholtz Centre for Ocean Research Kiel, Wischhofstrasse 1–3, 24148 Kiel, Germany
  • 2NIOZ – Royal Netherlands Institute for Sea Research, Department of Ocean Systems, and Utrecht University, P.O. Box 59, 1790 AB Den Burg, Texel, the Netherlands
  • 3HGF MPG Joint Research Group for Deep-Sea Ecology and Technology, Alfred Wegener Institute Helmholtz Centre for Polar and Marine Research, Am Handelshafen 12, 27570 Bremerhaven, Germany
  • 4Max Planck Institute for Marine Microbiology, Celsiusstrasse 1, 28359 Bremen, Germany

Correspondence: Laura Haffert (

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Deep-sea mining for polymetallic nodules is expected to have severe environmental impacts because not only nodules but also benthic fauna and the upper reactive sediment layer are removed through the mining operation and blanketed by resettling material from the suspended sediment plume. This study aims to provide a holistic assessment of the biogeochemical recovery after a disturbance event by applying prognostic simulations based on an updated diagenetic background model and validated against novel data on microbiological processes. It was found that the recovery strongly depends on the impact type; complete removal of the reactive surface sediment reduces benthic release of nutrients over centuries, while geochemical processes after resuspension and mixing of the surface sediment are near the pre-impact state 1 year after the disturbance. Furthermore, the geochemical impact in the DISturbance and reCOLonization (DISCOL) experiment area would be mitigated to some degree by a clay-bound Fe(II)-reaction layer, impeding the downward diffusion of oxygen, thus stabilizing the redox zonation of the sediment during transient post-impact recovery. The interdisciplinary (geochemical, numerical and biological) approach highlights the closely linked nature of benthic ecosystem functions, e.g. through bioturbation, microbial biomass and nutrient fluxes, which is also of great importance for the system recovery. It is, however, important to note that the nodule ecosystem may never recover to the pre-impact state without the essential hard substrate and will instead be dominated by different faunal communities, functions and services.

1 Introduction
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This work is part of the Joint Programming Initiative Healthy and Productive Seas and Oceans (JPI Oceans) MiningImpact project, which is an integrated research project assessing the potential ecological impact caused by deep-sea mining for polymetallic nodules. Most of these nodules occur in the deep ocean basins at water depths greater than 4000 m, which represents a part of the ocean that is largely unstudied. In fact, until recently it was assumed that life would be sparse in the deep ocean, where, in the absence of light, the only energy source is falling organic matter produced in the photic zone. Increased research interest and technological advances to overcome the large distances and pressure differences of over 400 times the atmospheric pressure have revealed that the deep sea hosts a surprising diversity of life forms. Information on benthic ecosystem functions, including spatial heterogeneity, temporal variability and biogeochemical feedbacks, are still limited but are an important prerequisite in the evaluation of the environmental impacts of deep-sea mining.

Figure 1Bathymetric map of the DEA region in the Peru Basin including the sampling stations presented in this work. More details on the sampling locations are provided in Table 1.

This work focuses on a unique study area in the Peru Basin (Fig. 1), where in 1989 a circular area with a diameter of about 2 nmi (nautical miles) was intensely disturbed with a plough–harrow as part of the DISturbance and reCOLonization (DISCOL) experiment (Thiel and Schriever, 1990). The DISCOL experimental area (DEA) was revisited before and several times after the initial disturbance, with research concentrating mainly on the characterization and distribution of benthic fauna (Borowski and Thiel, 1998; Borowski, 2001; Bluhm, 2001). Macrofaunal communities, which are particularly important for the bioturbation and thus redox zoning of the sediment, are mainly composed of Polychaeta (about 50 %) and to a lesser extent Tanaidacea (10 %–20 %) and Bivalvia (5 %–10 %; Borowski and Thiel, 1998). After the artificial disturbance, very few faunal groups returned to baseline or control conditions after more than 2 decades (Jones et al., 2017), and the main mode of macrofaunal recolonization was found to be via lateral migration (Borowski and Thiel, 1998).

The current MiningImpact project makes use of the technological advances that have taken place in recent years, e.g. precise underwater positioning and in situ experimentation by remotely operated vehicles (ROVs), in situ oxygen measurement, precise sampling with TV-guided or ROV-manipulated equipment and analysis of microbial functions, and attempts to derive a holistic understanding of the deep-sea mining impact on the closely linked ecosystem functions. The specific objectives of this work are as follows:

  • i.

    creating a biogeochemical (diagenetic) reference model for the DEA region and validating it based on a comprehensive set of geochemical data (pore water and solid phases from undisturbed and disturbed sediments) obtained with state-of-the-art methodology

  • ii.

    determining short- to long-term impacts of a deep-sea mining event on the geochemical system through field-data-supported simulations

  • iii.

    advancing our understanding of the intricate interplay between ecosystem functions and geochemical processes in the context of a benthic disturbance in the deep sea.

Figure 2Schematic diagram (not to scale) outlining the formation of a Fe(II)-rich clay phase (green) in the context of glacially high organic-matter input and its conversion to Fe(III) by downward progressing NO3 fronts during interglacial periods triggered by reduced organic-matter input as postulated by König et al. (2001). In this study, we propose that a second reaction front exists impeding the downward O2 migration through a reaction with the semi-labile Fe(II) phase.


2 Methods
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2.1 Biogeochemical site description

In comparison to other abyssal areas, the Peru Basin receives high organic-matter input, which is fuelled by the equatorial high-productivity zone (Weber et al., 2000). The degradation of deposited organic matter plays a crucial role in defining the benthic biogeochemical system (Froelich et al., 1979). The reactions utilize different terminal electron acceptors of the order of decreasing free-energy production, namely oxygen, nitrate, manganese oxide, iron oxide and sulfate, and their availability controls the position of the various redox zones in the sediment column. König et al. (1999, 2001) and Haeckel et al. (2001) have previously identified and quantified, through diagenetic modelling, the main biogeochemical processes in the Peru Basin, including the DEA region. They found that organic-matter degradation progresses through the stages of oxic respiration, denitrification, manganese and in deeper sediments iron oxide reduction before the reactive fraction of organic matter are diminished, leaving only the most refractory fraction of organic material to be permanently buried. Unlike manganese, the reduced Fe(II) species is not released into the pore water but instead incorporated into the clay mineral phase as nontronite, resulting in dissolved Fe concentrations below or near the detection limit. The largest quantity of organic matter is degraded by oxic respiration in a surface “reaction layer” which coincides with the bioturbated zone. Oxygen is depleted in the Peru Basin at a sediment depth of 5 to 15 cm, the latter being the typical oxygen penetration depth within the DEA region (Haeckel et al., 2001). Larger variations were found among the nitrate profiles. In the slightly shallower region of the Peru Basin, seawater-derived nitrate and nitrate from oxic respiration are consumed within the bioturbated upper 20 cm of sediment. In contrast, in the DEA region nitrate escapes the reaction layer and diffuses to a depth of about 2 m, where a Fe(II)-rich layer impedes further downward migration (König et al., 2001; see also Fig. 2). This Fe(II)-rich layer was formed during glacial times (corresponding to an age of at least 60 ka), when organic-matter deposition was strongly increased and potential electron acceptors such as oxygen, nitrate and manganese oxide were exhausted closely below the sediment surface (König et al., 2001). During these times, structural Fe(III) within smectite lattices acted as the thermodynamically favoured electron acceptor, creating Fe(II)-rich surface sediments that were subsequently buried (Lyle, 1983). This process was reversed when waning organic-matter input during interglacial periods allowed oxygen and nitrate to migrate downwards, thus creating a “burn-down” situation of the redox-sensitive Fe(II)-rich smectite phase (Fig. 2). The position of the reaction front is easily distinguished in deep sediment cores as a transition from a tan to green colour change (Lyle, 1983) and has also been confirmed by Mössbauer spectroscopic measurements (Drodt et al., 1997; König et al., 1997, 1999).

Comparatively little is known about the redox potential of Fe(II) on different crystallographic lattice positions (coordination sites) in clay minerals such as nontronite. In particular, information is lacking on the reactivity of the Fe(II) on these sites with respect to oxidation by nitrate and oxygen. Available Mössbauer data (König et al., 2001) from the DISCOL area indicate a refractory fraction of Fe(II) that prevails in the nitrate reduction zone (∼10 % of total iron). We propose that part of this refractory fraction is in fact semi-labile and that oxygen, which has a higher redox potential than nitrate, can oxidize this semi-labile Fe(II) and thus presents a second reaction front impeding the downward migration of oxygen.

2.2 Disturbance experiments

The current study presents information collected in the DEA during the two legs of the SO242 research cruise in 2015. The DISCOL experiment was designed to artificially disturb the surface sediment layer through mixing and remove nodules from the surface with a specially designed device, the so-called plough–harrow (Thiel and Schriever, 1990). To this end the ∼4150 m deep circular DEA (11 km2) was crossed 78 times starting from various directions, resulting in a heavily disturbed area in the centre and less disturbed peripheral regions. In total about 20 % of the DEA was directly ploughed, with the remaining 80 % being not directly disturbed but – at least in part – covered by an up to 30 mm thick resettled sediment blanket (Schriever and Thiel, 1992).

During the first leg of SO242 an additional artificial disturbance was created using an epibenthic sled (EBS) which was sampled 5 weeks later on the second leg of SO242. The epibenthic sled (CliSAP-Sled; Brenke, 2005) has a width of 2.4 m and weight of 880 kg (in air). Unlike the plough–harrow, which “mixes” the upper sediment layer, the EBS largely scrapes off the upper centimetres of sediment (most of the reactive, bioturbated layer), including embedded nodules, and bulldozes them sideways from the track.

Table 1Sampling station locations and description.

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2.3 Sampling procedure

A map of the sampling stations and a table of the exact positions are provided in Fig. 1 and Table 1, respectively. Several devices were deployed at each sampling site to retrieve surface and subsurface sediment samples (Greinert, 2015; Boetius, 2015): (i) a multiple corer (MUC, Oktopus, Kiel, Germany), which samples the upper 20–40 cm of the sediment including the overlying bottom water and was equipped with a TV–camera system for precise positioning, e.g. for sampling specific seafloor features like the disturbance tracks, (ii) a ROV-deployed push corer (PUC) focusing on small-scale topographic features created by the seafloor disturbance, (iii) a box corer (BC – USNEL type; Hessler and Jumars, 1974) to sample the sediment including macrofauna and nodules, and (iv) a gravity corer (GC) extracting sediment up to 10 m b.s.f.

After core retrieval the samples were brought into the ship's cold room (approx. 4 C); 1 m long sections of the gravity cores were cut into two half-cylinders dedicated for sampling and archiving. The working half of the gravity cores were sampled by extracting 3 cm thick slices at intervals of 20 to 40 cm. Sediment from the plastic liners of the other corers were extruded with a piston and cut into 0.5 to 2 cm thick slices under an oxygen-free, argon atmosphere in a glove bag. Subsequently, the pore water was extracted using a low-pressure squeezer (argon gas at 3–5 bar) and filtered through a Nuclepore 0.2 µm polycarbonate filter. Pore water extracted from the samples was stored in two recipient vessels: (i) an acidified sample (i.e. pH < 1 with 20 µL 30 % HCl Suprapur per 1 mL sample) for analysis of metal cations and (ii) a non-acidified sample for nutrients. Additional sediment samples were collected from the same core and sediment intervals for porosity, organic carbon content and radiometric analysis.

2.4 Analytical methods

Analyses for the pore water solutes NO3-, NO2-, NH4+, PO43- and SiO44- were completed onboard using a Hitachi UV/VIS spectrophotometer on 1.3 mL of freshly extracted untreated pore water which was diluted 3 fold before analysis. The respective chemical analytics followed standard procedures (Grasshoff et al., 1999); i.e. nitrite and nitrate (after reduction with Cd) were measured as sulfanile-naphthylamide, ammonium was measured as indophenol blue, phosphate and silicate as molybdenum blue, and iron with ferrospectral. The total alkalinity of the pore water was determined by titration with 0.02 N HCl using a mixture of methyl red and methylene blue as an indicator. The titration vessel was bubbled with argon to strip any CO2 produced during the titration. The IAPSO seawater standard was used for calibration. Further details on analytical methods, e.g. analytical precision and accuracy, are given in Table 2.

Table 2Summary of analysed properties, analytical methods, estimated analytical errors and detection limits.

a For some properties no analytical error could be determined because of few data points or concentrations close to the detection limit. b Grasshoff et al. (1999). c Welicky et al. (1983). d Breland and Byrne (1993).

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At the NIOZ laboratory sediment profiles of 210Pb and 226Ra, radioisotopes from the 234U decay series were measured in multicores for determining biological mixing rates integrated over a ∼100-year timescale. Total 210Pb and 226Ra activity was determined directly by gamma spectrometry, whilst total 210Pb was also measured indirectly by alpha spectrometry via its granddaughter isotope 210Po. Activities of anthropogenic 137Cs proved to be generally below the detection level. Radionuclide activities are reported in the database as millibecquerels per gram of dry sediment.

For gamma spectrometry, a few grams of a freeze-dried and homogenized sediment sample was contained in a 5 cm diameter plastic petri dish, which was closed with tape and sealed gas-tight in a plastic envelope. After leaving the sample for at least 4 weeks to ensure equilibrium, measurement of 210Pb and 226Ra was undertaken with a Canberra Broad Energy Range High Purity Germanium (BEGe) detector, using the 46.5 keV line for 210Pb and 295.2, 351.9 and 609.3 keV lines for 226Ra. The detector, connected to a computer via a digital spectrum analyser (DSA-1000), counted the radionuclide activities with Genie 2000 gamma spectroscopy software. The detector was externally calibrated with a Geological Certified Reference Material IAEA/RGU-1, with a reference date of 1 January 1988. A monitor standard IAEA-300 provided quality control. Excess 210Pb activities were calculated by subtracting 226Ra activity averaged for the 295.2, 351.9 and 609.3 keV lines from the measured total 210Pb activity.

For alpha spectrometry measurement of 210Po, 0.5 g of a freeze-dried and homogenized sediment sample was spiked with 1 mL of a standard solution of 209Po in 2 M HCl and then leached for 6 h in 10 mL of concentrated HCl heated to 85 C. After diluting the fluid with 45 mL of demineralized water and adding 5 mL of an aqueous solution of ascorbic acid (40 g L−1), natural 210Po and added 209Po were collected from the fluid by spontaneous electrochemical deposition on silver plates. For subsequent alpha spectrometry, Canberra Passivated Implanted Planar Silicon detectors were used. 210Pb activity was calculated from 210Po, assuming secular equilibrium and correcting for the time elapsed since collection of the samples.

2.5 Numerical methods

We follow the classical approach in early diagenetic modelling, where partial differential equations represent the diffusive and advective fluxes coupled to a reaction term (R), as formulated by Berner (1980). When applying a conversion via the volume fraction, the following expressions for solutes and solids can be derived, respectively:




where t and x represent time and the depth under the seafloor, respectively, Cpw and Cs are the molar species concentration of solute or solid, respectively, ϕ is the porosity, and u and w represent fluid and sediment velocity due to burial. Solute diffusion and sediment bioturbation are scaled by the effective diffusion coefficient D and the bioturbation coefficient Db.

The porosity–depth profile is assumed to be produced by steady-state compaction (Berner, 1980) and is approximated empirically by the following exponential function (Murray et al., 1978; Berner, 1980; Martin et al., 1991; Rabouille and Gaillard, 1991a, b):


where ϕ is the porosity at infinite depth, ϕ0 is the porosity at the sediment surface (x=0) and β is the porosity attenuation coefficient.

The fluid and sediment velocity–depth distribution is calculated according to the mathematical convention of Luff and Wallmann (2003):




where w is the burial velocity at infinite depth.

The effective diffusion coefficient was tortuosity corrected:


with D0 being the infinite-dilution molecular diffusion coefficient calculated after Berner (1980) and θ2 being the squared tortuosity. The tortuosity can be related to the porosity ϕ according to Boudreau (1996):


The bioturbation profile is approximated by the arbitrary function:


where Db0 represents the maximum bioturbation intensity at the sediment surface, xDb is the bioturbation half-depth and βDb is the bioturbation attenuation coefficient.

The diagenetic equations, consisting of a set of partial differential equations (Eqs. 1 and 2), are solved via a finite-difference scheme (1-D uneven grid with a sigmoidal distribution ranging from 0.1 cm at the surface to 1 cm at the lower boundary) and subsequent minimization of the ordinary differential equations by inbuilt ODE (ordinary differential equation) solvers of MATLAB.

Table 3Reaction stoichiometry and rate expressions of the organic mater (CH2OaNH3bH3PO4c) degradation and secondary redox reactions. All solute species are in concentrations of millimoles per litre of porewater (mmol Lpw-1) and all solid species in millimoles per litre of dry sediment (mmol Lds-1). All reaction rate expressions are stated (in the units of mmol Lpw-1 a−1) via a conversion with Fpw=1-ϕϕ.

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The reaction network shaping the geochemistry of the upper sediment metres, namely organic-matter degradation and secondary redox reactions including the oxidation of ammonium and dissolved manganese, is listed in Table 3 as well as the corresponding rate expressions. To account for different reactivities of the various organic-matter phases, we apply a 3G model (Jorgensen, 1978; Westrich and Berner, 1984; Middelburg, 1989) allowing for a labile, moderately reactive and refractory phase. We also included a shallow semi-labile Fe(II) layer that reacts with oxygen to the model (Fig. 2). While it is established that a labile Fe(II) reaction front impedes the downward migration of nitrate (König et al., 1997, 1999), little is known about the redox potential of Fe(II) on different crystallographic lattice positions (coordination sites) in clay minerals such as nontronite. In particular, information is lacking on the reactivity of the Fe(II) on these sites with respect to oxidation by nitrate and oxygen. Available Mössbauer data (König et al., 2001) from the DISCOL area indicate a refractory fraction of Fe(II) that prevails in the nitrate reduction zone (∼10 % of total iron). In the light of the much higher redox potential of oxygen compared to nitrate, we model the remaining Fe(II) as a semi-labile phase representing a second reaction front impeding the downward migration of oxygen. The diagenetic model is applied to define a background or reference geochemical system. This is then used to derive transient prognostic models in response to different impact types. Initial profiles of the transient simulations are based on the steady-state background model which was adjusted for the modelled impact type: (i) in the case of “sediment removal”, the top 10 cm of the background profiles was “cut off” while maintaining bottom water concentrations and organic-matter flux for the upper boundary conditions. For the “sediment mixing” case, the oxygen concentrations of the upper 10 cm of the sediment were set to bottom water values, imitating an oxygen flooding event. Transient models were augmented by an additional Fe(II) profile – an oxygen reaction layer (Fig. 2) – as justified by the surprisingly constant oxygen penetration depth at the reference and all disturbed sites. This Fe(II) reaction layer is crucial in the prognostic simulations which would otherwise predict that oxygen rapidly diffuses into deeper sediments after a disturbance event. It is assumed that bioturbation is inhibited immediately after the impact, with a linear increase to undisturbed reference bioturbation intensity within 100 to 200 years, which is in line with macrofaunal abundance data presented in Stratmann et al. (2018a). A detailed parameterization of the background and prognostic models is provided in Table 4.

The bioturbation coefficient Db0, a critical input parameter to the background model, is derived by fitting a simplified version of the background model to summed 210Pb, 226Ra and 230Th radiometric data. The physical set-up of the model is the same as the background model, with the difference that only the above-mentioned radiometric species and their first-order radioactive decay is considered.

Figure 3Organic carbon content (Paul et al., 2018, 2019) and dissolved solute profiles of summed NO3 and NO2 (Paul et al., 2018, 2019), NH4 and Mn in gravity cores, and their corresponding multicores. Symbols represent the measured values at the mean depth of the sediment layer sampled. Buried manganese nodules are indicated by a grey line. Details on individual sampling stations can be found in Table 1.


3 Results
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3.1 Geochemistry of the DEA region

The parameter depth profiles for each core are presented in Figs. 3–6, grouped into “outside DEA” and “inside DEA” stations for the gravity cores and for the multiple and box cores in reference sites and various types of disturbed sites.

Figure 4Porosity and organic carbon content in multiple and box cores retrieved in various microhabitats (Paul et al., 2018). Symbols represent the measured values at the mean depth of the sediment layer sampled. Details on individual sampling stations can be found in Table 1.


Table 4Constrained and fitted parameters used in the numerical simulations.

(a) Approximated by field data. (b) Fitted to Pb data (Fig. 7). (c) Fitted to field data. (d) Approximated from data and references presented in Volz et al. (2020). (e) Inferred from data presented in König et al. (1997, 1999).

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Figure 5Averaged (black line) in situ oxygen profile representative of various disturbance settings including their standard deviations (shaded area). Because the depth steps between each measuring point of the oxygen profiles are very close, they are not resolved in the diagram. Details on individual sampling stations can be found in Table 1.


Geochemical trends observed in the sediment cores from reference stations agree in general well with those observed in the same area during a previous investigation (Haeckel et al., 2001). The upper reactive sediment section (up to ∼20 cm b.s.f) is markedly different from the deeper sediment sections (up to 10 m; Fig. 3). It is characterized by a significant decrease in porosity (from 0.94 to approx. 0.86; Table 4 and Fig. 4) and is rich in manganese oxides (1–2 wt %; Paul et al., 2019), which gives this layer its distinct dark brown colour. Below this layer, the sediment takes on a light brown to grey brown (tan) colour, which is replaced by a greyish–green colour – the shift to Fe(II)-rich lattices in the smectite phase – at a depth of 2–2.5 m (38 GC 1, 84 GC 3, 100 GC 5 and 123 GC 6; Table 1). Organic carbon content in the DEA region oscillates about a mean value of 0.5 to 0.75 wt % in the upper 50 cm (Fig. 3), which is controlled by the difference in organic carbon input during glacial (higher Corg content) and interglacial (lower Corg content) sedimentation regimes (Haeckel et al., 2001). With depth the organic carbon content decreases steadily to about 0.1 wt % at 10 m.b.s.f (Fig. 3). Contrary to previous observations that nodules in the DEA area occur only at the sediment surface, investigation of the current set of gravity cores revealed buried manganese nodules at depths of 4 to 8 m (Fig. 3). The undisturbed appearance of the sediment surrounding these buried nodules, and evidence of diagenetic alteration of the nodules, proves that these nodules were genuinely buried and do not represent a coring artefact.

Figure 6Solute profiles in multiple and box cores retrieved in various microhabitats. Symbols represent the measured values at the mean depth of the sediment layer sampled (summed NO3 and NO2 from Paul et al., 2018). Details on individual sampling stations can be found in Table 1.


Figure 7Model results of the faunal bioturbation activity in reference and DEA outside track sediments based on measured 210Pb activities. Left: model curves indicate total 210Pb activity (solid) and supported contributions by 230Th (dotted) and 226Ra (dashed). Right: bioturbation intensity profile derived for the background model.


In situ oxygen profiles confirm that the upper brown layer represents the oxygenated zone (Fig. 5). In this zone, oxic respiration of organic matter not only consumes downward diffusing oxygen but also increases ammonium and, via nitrification, nitrate (Fig. 6). While the trend in the nitrate profiles is clearly discernable, ammonium concentrations remain rather low (10–20 µmol L−1) and are scattered and likely controlled by secondary processes, such as adsorption and desorption (Haeckel et al., 2001). Below the oxic dark brown layer, denitrification commences and nitrate declines within the upper 2–3 m of the sediment and is entirely depleted in the cores with the greyish green Fe(II)-rich reaction layer (51GC2, 84GC3, 100GC5, 123GC6; Fig. 3). The depleted nitrate profiles tend to be more linear (e.g. 84GC3) than the profiles of cores where the Fe(II)-rich layer is absent (e.g. 132GC7), as is typical in the presence of a reaction layer (Goloway and Bender, 1982; Wilson et al., 1985; Jahnke et al., 1989). Dissolved manganese is strongly redox sensitive and is absent in the upper oxic zone (Fig. 6). Below the oxic zone, organic-matter degradation progresses to manganese oxide reduction and produces dissolved manganese, which increases steadily until it reaches an asymptotic concentration at 6 m depth, with terminal concentrations ranging between 25 and 120 µmol L−1 (Fig. 3). Diagenetic simulations (Haeckel et al., 2001) could not reproduce this strong increase in dissolved manganese, and the discovery of buried manganese nodules now confirms that the dissolved manganese concentrations are indeed additionally shaped by the dissolution of manganese nodules at a depth of 4–8 m.

Alkalinity profiles are largely unchanged over the entire sampling domain (up to 10 m) but display some variations in the upper 5 cm (Fig. 6). Haeckel et al. (2001) have argued that the alkalinity profile is likely influenced by carbonate geochemistry, so we will neglect the upper alkalinity profile in our interpretations. Dissolved iron concentrations were near or below the detection limit of 1 µM in all cores due to its incorporation into the clay mineral phase, and sulfate concentrations remained close to their sea water value, which is in line with the absence of sulfate reduction in the Peru Basin.

3.2 Diagenetic reference model

With the MiningImpact sampling campaign specifically targeting the DEA region, we were able to update the parameterization of the previous model presented by Haeckel et al. (2001). The main differences are as follows. (i) The presented model applies a 3G model, i.e. allows for three fractions of organic matter with varying reactivity and thus a better fit to the field data. (ii) The bioturbation intensity (Db=0.65 cm2 a−1) and depth profile are now based on radioactive disequilibria between radionuclides of the natural uranium and thorium series in the DEA region (Fig. 7). The numerical simulations predict that excess 210Pb (difference between solid and dashed lines) exists down to ∼10 cm and below the measured activity is supported by 230Th (dotted line) and 226Ra (difference between dashed and dotted lines). A relatively high scatter of radioactive activities of 1.5–3 Bq g−1 was observed in the uppermost sediment samples (0–0.5 cm), reflecting lateral inhomogeneities. These could be due to sediment focusing created by local topography, e.g. small depressions acting as deposition centres for fluffy material, or by “hotspots” of non-local mixing by individual organisms.

A satisfactory background model fit to undisturbed reference sites could be achieved for all relevant parameters, with particular focus being placed on accurately simulating the redox zonation of the sediment, especially the oxygen penetration depth and the concurrent increase in dissolved manganese (Fig. 8). It should be noted that the geochemical state of the Peru Basin is likely transient in nature, arising from long-term (glacial and interglacial) as well as short-term (ENSO timescale) variations in the depositional flux of organic matter (König et al., 2001). This work focuses on modelling the biogeochemical processes in the upper half-metre of the sediment; these are defined by the Holocene organic-matter input and are thus unaffected by long-term changes in the depositional flux. Similar to the downward-migrating nitrate front, it is likely that the pore water oxygen content also increases with currently waning organic-matter input. The analytical oxygen profiles display less curvature (more linearity) than the modelled profiles, which suggests that secondary reactions, such as a semi-labile Fe(II)–oxygen reaction front, additionally shape the oxygen profile. However, due to the uncertainty in a transient parameterization, we model the reference sediments as a steady-state scenario – justified by the generally good agreement of the model with the geochemical data.

3.3 Impact characterization

All sample stations were affected by at least one of the following processes: (i) removal of the upper dark brown reactive layer, (ii) mixing and redistribution of the surface sediment, resulting in piling up (“ridges”) and removal (“furrows”), (iii) turning of the upper sediment upside down or bringing up deep sediment to the surface – both leading to exposure of sediment from below the reactive layer at the surface, and (iv) deposition of resuspended sediment. Furthermore, there were distinct differences in the physical impact of the plough–harrow and EBS track. While the EBS scraped off the surface sediments and relocated them to the side of the track, the plough–harrow mixed and displaced sediments, resulting in a variety of disturbance structures. In the following a detailed description of the physical and geochemical impact is provided and will be complemented by information on the microbiological impact from a parallel study (Vonnahme et al., 2020).

Sediments inside the EBS track are largely devoid of the upper reactive brown layer, exposing the tan-coloured subsurface sediment in places. The surface of the track is smooth, indicating that sediment mixing is negligible inside the tracks. Removal of the upper reactive layer resulted in decreased sediment porosity by exposing more compacted sediment compared to the reference sites and brought the boundary below which dissolved manganese is found in the sediment pore water as close as 5 cm b.s.f. (Figs. 4 and 6). In contrast, the oxygen penetration depth does not vary significantly from the reference area; the profile shape is, however, slightly more linear (Fig. 5). In line with the severe disturbance of the sediment, physical signs of bioturbation (recent bioturbation channels connected to the surface) are lacking and the microbial cell count in the surface sediment is reduced by 50 %, with the microbial communities being significantly different from the reference sites (Vonnahme et al., 2020).

The disturbance of the surface sediment layer brought about during the DISCOL experiment was still clearly visible after 26 years, and the TV-guided sampling campaign was able to specifically target the following features. (i) The area next to the plough–harrow track (outside track) is not mechanically disturbed but affected by resettling sediment from the disturbance plume. Geochemical profiles do not indicate a significant difference to the reference site (Fig. 6), especially with the natural heterogeneity of the area in mind. Differences in the microbial functions compared to the reference stations are also within the methods' confidence level, and recent bioturbation confirms that the sediment is presently colonized by burying macrofauna (Vonnahme et al., 2020). (ii) The furrows drawn in the seabed by the plough–harrow show some distinct differences to the reference sites. The reactive dark brown surface layer is reduced in thickness and is overlain by resettling sediment which preferentially accumulated in the depression. While geochemical profiles do not differ significantly from the reference site, biological processes are noticeably decreased (Vonnahme et al., 2020). (iii) Ridge sediments show the same disturbance effects, albeit more severe, and seafloor integrity is compromised by deep cracks throughout the sediment (Vonnahme et al., 2020). (iv) Exposed subsurface sediment observable as lighter-coloured patches shows the highest level of disturbance, similar in character to the fresh surface created by the EBS. The reactive brown layer is absent, and thus the porosity of the surface sediment reduced (Fig. 4). Biological processes are significantly impacted. Bioturbation channels are scarce and disconnected with the surface, indicating a pre-impact origin. Microbial cell numbers are reduced by 30 %, with the microbial communities being more similar to those of the subsurface sediment (14–16 cm b.s.f.) of the reference stations than those of the surface sediments (Vonnahme et al., 2020).

Figure 8Simulation of background biogeochemical processes and their effect on solute profiles in the DEA region. In addition to the three reference stations, two other undisturbed sites were included for comparison. See Table 4 for parameterization of the modelled profiles.


3.4 Prognostic simulations

The following presentation of simulation results focuses on the distribution of oxygen, which plays a central role in biogeochemical processes: different impact types were simulated by two “endmember” scenarios. The first scenario assumes that 10 cm of the upper sediment were removed; i.e. the first 10 cm of the reference steady-state profiles were discarded, exposing anoxic subsurface sediments to bottom water concentrations. The second scenario floods the upper 10 cm of the sediment with oxygen, representing, on the one hand, the effect of sediment mixing and cracking and, on the other, the effect of resuspension and subsequent settling of freshly oxygenated reactive sediment (Fig. 9). The transient models showed that the impact of the removal of the reactive upper 10 cm layer is much more severe and longer lasting compared to the “oxygen flooding” scenario. And simulations show no distinguishable difference if 8 or 12 cm is removed instead of 10. This is because the greatest disturbance effect is caused by the absence of the labile organic-matter fraction, which is restricted mostly to the upper 10 cm of the sediment.

Figure 9Schematic characterization of the different impact types at a mined seafloor and their influence on O2 distribution.


Short-term impact

Immediately after the removal of the highly reactive surface sediment, solute profiles are in strong disequilibrium and mostly diffusion controlled. This is especially true for oxygen, which rapidly diffuses into previously anoxic sediments. The Fe(II) layer plays an insignificant role in the initial post-impact weeks but commences to inhibit further penetration of the oxygen front at 5 weeks. In the absence of the reactive surface layer, organic-matter degradation rates, which strongly shape the reference oxygen profile, play only a minimal role in the weeks after the disturbance. The modelled “removal” profiles agree well with the analytical in situ oxygen profiles of the 5-week-old EBS track and confirm that the linear nature of the analytical profiles is likely shaped by a mixture of a downward-diffusing oxygen front and the initiation of the oxygen–Fe(II) reaction layer. For comparison, impact simulations assuming full recovery of the bioturbation intensity by 200 years instead of 100 years are also shown. Here, the oxygen profile is also distinctly more linear, but even more reduced sediment mixing through bioturbation allows oxygen to penetrate slightly deeper (18 cm b.s.f.) compared to the in situ oxygen profiles (13 cm) and to the 100-year bioturbation recovery interval (10 cm b.s.f, Fig. 10).

Figure 10Impact simulations after a disturbance that causes the reactive upper 10 cm to be removed. The short-term (up to 5 weeks, comparable to the EBS tracks) and medium-term (26 years, comparable to the DEA tracks) impacts are visualized by the respective O2 profiles and the fluxes shaping the O2 profiles. It is assumed that the oxygen penetration depth is impeded by a reactive Fe(II) layer. Note that the Fe(II) is assumed to be located at 20 cm b.s.f. but is placed at 10 cm if the upper 10 cm is removed. For comparison the O2 profile in the absence of a Fe(II) layer is also shown. The long-term impact is demonstrated by the surface flux of oxygen into the sediment, the depth-integrated reaction rates (DIRs) of oxygen with organic matter (Corg) and with the reactive Fe(II) layer, and the oxygen penetration depth (OPD) over time. The influence of the bioturbation recovery time is shown by plotting the lines that correspond to a recovery interval of 100 and 200 years in black and grey, respectively.


The oxygen flooding scenario produces a very different oxygen profile. It is shaped mainly by the reaction of the anomalous oxygen with the reactive (labile) organic matter, especially in the upper centimetres, where the labile organic matter is most concentrated. The difference to the EBS track in situ profiles supports the observation that the EBS track is mainly affected by sediment removal rather than mixing (Fig. 11).

Figure 11Impact simulations after a disturbance causing the upper sediment layer to be exposed to bottom water oxygen levels (i.e. through mixing or by resettling sediments; see Fig. 9). See the caption of Fig. 10 for details.


Medium-term impact

The existence of the Fe(II)–oxygen reaction layer plays a particularly important role in the decades after the disturbance. Oxygen is less efficiently removed from the pore water in both scenarios: removal of the reactive layer significantly reduces oxygen uptake through organic-matter degradation, and the reactivity of the organic matter was reduced due to reaction with the anomalous flooding with oxygen. In the absence of an oxygen-impeding reaction layer, oxygen penetration depths would consequently be significantly increased in both cases (Figs. 10 and 11). Simulating the 26-year-old DISCOL disturbance has shown that the existence of an oxygen-consuming reaction layer is a necessary prerequisite. The removal scenario produces slightly more linear profiles compared to the oxygen flooding simulation because of the relative importance of the Fe(II)–oxygen reaction layer over the organic-matter degradation process. The slight misfit of both scenarios with the in situ oxygen data suggests that the DEA tracks are most likely affected by a mixture of both scenarios, possibly levelled out by lateral diffusion effects. Ongoing organic-matter flux at the surface sediment builds up a new post-impact reactive layer with reactivities higher than in the reference model due to the ineffective mixing into deeper sediments through bioturbation (Figs. 10 and 11). Thus, differences between bioturbation recovery scenarios, i.e. 100 and 200 years, increase over time with an increasing amount of fresh organic matter that can potentially be mixed into the sediment.

Long-term impact

It takes centuries for the geochemical processes to completely recover after the removal of the upper reactive sediment. While the observed geochemical profiles of the disturbed sites show surprisingly little variation compared to the reference site, overall surface fluxes vary significantly during the first 100 years after the impact. This is especially true for the oxygen flux into the sediment, which is more than halved within the first year of the impact (Fig. 10). Within the first decades after the impact, the build-up of the labile organic matter, and its mixing into deeper sediment through bioturbation, gains influence. With increasing depletion of oxygen through organic-matter degradation, oxygen concentrations decrease along with Fe(II)–oxygen reaction rates. The transition from Fe(II)-controlled to organic-matter-controlled oxygen profiles is accompanied by a slight increase in the oxygen penetration depth (Fig. 10). The exact quantification of this transition is difficult to estimate, but the fact that 26 years after the impact oxygen profiles are still efficiently impeded by the Fe(II)–oxygen reaction layer suggests that this process will significantly reduce the oxygen penetration depth until the organic-matter degradation rates are near pre-disturbance levels (ca. 100 years after the impact). Interestingly, while steady-state biogeochemical processes strongly depend on the depth and intensity of bioturbation (Haeckel et al., 2001), the time frame of the bioturbation recovery has very little influence on the overall impact recovery process (Fig. 10). This is due to the fact that the limiting factor is the availability of labile organic matter, which is replenished on a much longer timescale compared to the faunal recovery (Stratmann et al., 2018a).

Recovery of the sediment that was affected by the oxygen flooding scenario occurs within 100 years and is already in a near-pre-impact state 1 year after the disturbance. Here, the Fe(II)–oxygen reaction layer is predominantly responsible for restricting the anomalously high oxygen levels within the upper 20 cm of the sediment. Organic-matter degradation rates remain high throughout the recovery process, and build-up of fresh labile organic matter that replenishes the loss through the introduction of anomalous oxygen concentration occurs within the recovery phase of the bioturbation (Fig. 11).

Figure 12(a) Schematic diagram showing the delicate interplay between the distribution of organic matter, microbial activity and the bioturbation through burrowing megafauna in a pre-impact system and after removal of the upper reactive sediment layer. (b) Quantitative comparison of geochemical and biological processes over time after an impact through sediment removal. Biological data are taken from Stratmann et al. (2018a; macrofaunal abundance) and Vonnahme et al. (2020; microbial cell count) for the most severely disturbed sites (e.g. inside 5-week-old EBS tracks and inside 26-year-old plough marks).


4 Discussion
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The benthic ecosystem is shaped by a balanced interplay of physical, chemical and biological factors, which are fuelled by the deposition of organic matter, especially its labile fraction (Fig. 12): the degradation of organic matter is microbially catalysed and in turn provides nutrients to the microbial community (e.g. Witte et al., 2003a; Moodley et al., 2005; Stratmann et al., 2018b; Sweetman et al., 2018). The associated microbial growth provides biomass that sustains micro- and macrofauna and forms an important component of the benthic food web (Witte et al., 2003b; Van Oevelen et al., 2006, 2011). The concomitant bioturbation of the sediment induces a variety of changes, such as lowering the sediment shear strength and the vertical transport of fresh reactive organic matter into deeper sediments (bioturbation) through burrowing invertebrates (Krantzberg, 1985, and references therein). The occurrence of labile organic matter in deeper sediments where oxygen is not as easily replenished compared to surface sediments places an important restriction on the oxygen penetration depth (Haeckel et al., 2001). Bioturbation plays thus a deciding factor in early diagenetic, especially redox-sensitive, processes. These are typically closely linked to the microbial functions and, with the introduction of fresh and digested organic matter at depth by infauna, new material for additional degradation and microbial colonization, close the causal cycle (Levin et al., 1997; Witte et al., 2003a; Middelburg, 2018). Changes in any compartment of the ecosystem functions will entail modifications of all biogeochemical processes. A natural example is the long-term glacial and interglacial or short-term ENSO-driven change in the depositional flux of organic matter to the seafloor, imposing gradual cycles in the redox zonation of the sediment (König et al., 2001).

Mining the seafloor will have an abrupt and direct impact on several benthic ecosystem functions at a time: (i) through the removal of all seafloor fauna (Vanreusel et al., 2016; Jones et al., 2017; Gollner et al., 2017; Bluhm, 2001; Borowski, 2001), (ii) through changing the physical characteristics of the sediment surface, i.e. by exposing more compacted sediments with increased shear strength, and removing manganese nodules as hard-substrate habitats (Grupe et al., 2001), and (iii) through the displacement and removal of labile organic matter.

Biological, geochemical and numerical results of the MiningImpact project significantly improve our understanding of the interlinked ecosystem recovery processes in the deep sea. Removal of the upper reactive surface layer and its fauna will halt bioturbation and organic degradation rates and significantly decrease microbial activity (Fig. 12a and b). Based only on deep-sea typical microbial biomass turnover rates and conservative bacterial doubling times, recovery of microbial abundances should occur within 2–10 years. Observations show, however, that the microbial activity remained significantly reduced in the decades following the DISCOL disturbance experiment (Vonnahme et al., 2020). Microbial recovery thus appears controlled by the re-establishment of the labile organic-matter fraction, which is still significantly reduced at this timescale. Low nutrient fluxes and the downsized microbial biomass cannot sustain pre-impact faunal abundances (Stratmann et al., 2018a), and recolonization is obstructed by the comparatively hard substrate and absence of hard-substrate habitats (nodule surface) in impacted areas (Vonnahme et al., 2020). This directly affects the macrofaunal efficiency to mix surface sediments into deeper layers, which is presented in the simulations by the reduced bioturbation coefficient (Fig. 12). It should be noted that even if sediment physical conditions and organic-matter availability have recovered, it is unclear whether the pre-impact infauna is still available to recolonize the disturbed area.

Prognostic simulations showed that the timescale of the ecosystem recovery ultimately depends only on one factor: the availability of labile organic matter. The amount of reactive organic matter determines the intensity of the food-web activity (i.e. summed carbon cycling; Stratmann et al. 2018a) and has to reach pre-impact levels to allow for the interlinked ecosystem to recover. In this context, two aspects should be particularly emphasized. (i) Deep-sea sediments are characterized by extremely low surface sedimentation rates (∼0.004 cm a−1) and thus organic-matter influx, so complete recovery of the labile organic-matter fraction takes up to 1000 years (Fig. 12). In the Clarion–Clipperton Zone, located also in the abyssal plains of the Pacific Ocean, surface sedimentation rates are even lower (0.0002–0.001 cm a−1; Volz et al., 2018). In this area, where nodule mining is expected to commence, potential impact recovery after removal of the upper reactive layer was predicted to occur at an even longer (millennia) timescale (Volz et al., 2020). (ii) Ecosystem impact and consequent recovery are strongly dependent on the type of disturbance, more precisely, on the amount of labile organic matter removed. Microbial activity at disturbed sites could be directly correlated to an arbitrary disturbance gradient (Vonnahme et al., 2020), which in turn coincides with the thickness of the reactive dark brown surface sediments that were removed during the experimental disturbance. These findings are in line with the results of the prognostic simulations, which showed that the impact is much less severe and that recovery times significantly reduced (decades; Fig. 11) if sediments are simply mixed or affected by resettling sediment (oxygen flooding scenario) compared to the removal of the upper reactive layer (centuries; Figs. 10 and 12).

5 Conclusions
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Previous diagenetic models of the DEA (Haeckel et al., 2001) were updated by a field-based bioturbation coefficient and application of a 3G organic-matter model. A near-surface Fe(II)–oxygen reaction layer was introduced in the prognostic simulations, which represents the refractory Fe(II) phase that did not react with the downward-progressing interglacial nitrate front. The surprisingly similar oxygen penetration depths in reference and 26-year-old disturbed sediments indicate that this refractory Fe(II) appears to be an efficient barrier for the progressing oxygen front, thus defying previous impact simulation that predicted a significant downward migration of the oxygen front (König et al., 2001).

Transient simulation results have significantly enhanced the quality of the interpretation of the observed geochemical profiles, which at first sight do not exhibit marked differences among the various disturbed and reference sites. The geochemical recovery after a mining-related removal of the upper reactive sediment layer can be divided into three stages: (i) the initial diffusion-driven equilibration of the post-impact profiles (within weeks of the impact), (ii) burning down of the Fe(II)–oxygen reaction layer (decades after the impact) and (iii) re-establishment of the reactive organic-matter layer (centuries after the impact). If the reactive surface sediment is not removed but instead mixed or temporarily resuspended, anomalously high oxygen concentrations alter biogeochemical processes on a decadal scale, albeit much less severely.

The interdisciplinary (geochemical, numerical and biological) approach to characterize the impact of benthic disturbances on early diagenetic processes provided valuable information on post-impact processes. On the one hand it identifies variables that are suitable as indicators of benthic ecosystem health and also allow identification of significant adverse change of the benthic environment. Metabolites of microbial activity were found to be particularly sensitive to disturbances and can even resolve grades of impact (Vonnahme et al., 2020). Biogeochemical processes responded more subtly to the disturbance, with oxygen, nitrite and bioturbation activity being the most suitable variables. On the other hand, this work highlights the closely linked nature of different benthic ecosystem functions, which is also true during the recovery from mining impacts. The main factor constraining the time frame of the geochemical recovery is the availability of reactive (labile) organic matter, emphasizing the importance of the impact type (sediment removal versus mixing and resuspension) and also the natural depositional flux of organic matter onto the sediment surface (e.g. DEA versus the Clarion–Clipperton Zone). The microbial degradation of the available reactive organic-matter fraction defines the base of the food web structure, which eventually sustains metazoans. Some of the epifauna burrows into the seafloor (bioturbation) and in turn has a critical influence on geochemical, especially redox-sensitive, processes and fluxes. Recovery of the system is thus only possible if the ecosystem functions of all compartments are restored. Our results also show that it is important to identify regional features, such as the reactive Fe(II) layer in DISCOL sediments, because they may affect the biogeochemical response to a benthic disturbance drastically.

While biogeochemical fluxes may recover close to the pre-impact state, the nodule ecosystem cannot recover, since the essential hard substrate, the polymetallic nodules, have been removed. Consequently, a new nodule-free ecosystem with very different faunal communities, functions and services has to be established in the mining areas and its thickly blanketed surrounding. Furthermore, this new ecosystem will take much longer timescales to establish because it is largely controlled by the recovery of the underlying biogeochemical fluxes and processes that fuel abyssal life.

Data availability
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Data availability. 

The geochemical data presented in Figs. 3, 4 and 6 are publicly available in the PANGAEA database with the DOI (Haeckel, 2019). The PANGAEA database also hosts the activity measurements of the 210Pb series presented Fig. 7, with the DOIs (de Stigter, 2019a, alpha spectrometry) and (de Stigter, 2019b, gamma spectrometry).

Author contributions
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Author contributions. 

LH prepared the paper with substantial contributions from all co-authors. MH devised this study and conducted the onboard coring, sampling and chemical analyses of presented solid phase and pore water data. FJ carried out the in situ oxygen measurements during expedition SO242/2 and contributed the data for model validations. HdS provided the 210Pb radioactive data series which allowed for the quantification of the bioturbation intensity. LH and MH conducted the biogeochemical model simulations.

Competing interests
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Competing interests. 

The authors declare that they have no conflict of interest.

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The authors are solely responsible for the content of this paper.

Special issue statement
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Special issue statement. 

This article is part of the special issue “Assessing environmental impacts of deep-sea mining – revisiting decade-old benthic disturbances in Pacific nodule areas”. It is not associated with a conference.

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The authors would like to thank Anke Bleyer, Regina Surberg, Bettina Domeyer and Kristin Hamann for co-working on sample collection on the RV Sonne and onboard and onshore pore water analyses. Axel Nordhausen, Tobias Vonnahme, Frank Wenzhöfer and Cäcilia Wigand are acknowledged for their contribution to in situ measurements during the RV Sonne expedition. Piet van Gaever is thanked for carrying out the radionuclide analysis. We are also indebted to the captain and crew of the RV Sonne for their invaluable support during the cruise SO242. We are grateful for the constructive comments of two anonymous reviewers.

Financial support
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Financial support. 

This work was funded by the German Federal Ministry of Education and Research through the MiningImpact project (grant nos. 03F0707A, 03F0707D, 03F0812A and 03F0812D) of the Joint Programming Initiative of Healthy and Productive Seas and Oceans (JPIO). In that same project context, Henko de Stigter received funding from the Dutch Research Council (NWO) (grant no. 856.14.003).

The article processing charges for this open-access
publication were covered by a Research
Centre of the Helmholtz Association.

Review statement
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Review statement. 

This paper was edited by Jack Middelburg and reviewed by two anonymous referees.

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Publications Copernicus
Short summary
Deep-sea mining for polymetallic nodules is expected to have severe environmental impacts. Through prognostic modelling, this study aims to provide a holistic assessment of the biogeochemical recovery after a disturbance event. It was found that the recovery strongly depends on the impact type; e.g. complete removal of the surface sediment reduces seafloor nutrient fluxes over centuries.
Deep-sea mining for polymetallic nodules is expected to have severe environmental impacts....