Revisiting chlorophyll extraction methods in biological soil crusts – methodology for determination of chlorophyll a and chlorophyll a + b as compared to previous methods

Caesar, Jennifer; Tamm, Alexandra; Ruckteschler, Nina; Leifke, Anna Lena; Weber, Bettina

doi:https://doi.org/10.5194/bg-15-1415-2018

Articles | Volume 15, issue 5

https://doi.org/10.5194/bg-15-1415-2018

© Author(s) 2018. This work is distributed under
the Creative Commons Attribution 4.0 License.

Special issue:

Biological soil crusts and their role in biogeochemical processes...

https://doi.org/10.5194/bg-15-1415-2018

© Author(s) 2018. This work is distributed under
the Creative Commons Attribution 4.0 License.

Articles | Volume 15, issue 5

Research article

|

08 Mar 2018

Research article |

| 08 Mar 2018

Revisiting chlorophyll extraction methods in biological soil crusts – methodology for determination of chlorophyll a and chlorophyll a + b as compared to previous methods

Jennifer Caesar, Alexandra Tamm, Nina Ruckteschler, Anna Lena Leifke, and Bettina Weber

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Final revised paper (published on 08 Mar 2018)
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Preprint (discussion started on 25 Sep 2017)
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Interactive discussion

Status: closed

AC: Author comment | RC: Referee comment | SC: Short comment | EC: Editor comment

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RC1: 'Referee comments', Jayne Belnap, 18 Oct 2017
- AC1: 'Response to comments of reviewer 1 for manuscript: Caesar et al.: Revisiting chlorophyll extraction methods in biological soil crusts —methodology for determination of chlorophyll a and chlorophyll a+b as compared to previous methods', Jennifer Caesar, 27 Dec 2017
RC2: 'comments reviewer 2', Kees Jan van Groenigen, 29 Nov 2017
- AC2: 'Response to comments of reviewer 2 for manuscript: Caesar et al.: Revisiting chlorophyll extraction methods in biological soil crusts —methodology for determination of chlorophyll a and chlorophyll a+b as compared to previous methods', Jennifer Caesar, 27 Dec 2017

Peer-review completion

AR: Author's response | RR: Referee report | ED: Editor decision

ED: Publish subject to minor revisions (review by editor) (30 Dec 2017) by Kees Jan van Groenigen

AR by Jennifer Caesar on behalf of the Authors (13 Jan 2018) Author's response Manuscript

ED: Publish as is (24 Jan 2018) by Kees Jan van Groenigen

AR by Jennifer Caesar on behalf of the Authors (30 Jan 2018) Manuscript

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Short summary

In our study we analyzed the efficiency of different chlorophyll extraction solvents and investigated the effect of different preparatory steps to determine the optimal extraction method for biological soil crusts. Based on our results we confirm a DMSO-based chlorophyll extraction method without grinding pretreatment and suggest to insert an intermediate shaking step for complete chlorophyll extraction.