Abstract. Carbon (C) turnover time is a key factor in determining C storage capacity in various plant and soil pools as well as terrestrial C sink in a changing climate. However, the effects of C turnover time on ecosystem C storage have not been well explored. In this study, we compared mean C turnover times (MTTs) of ecosystem and soil, examined their variability to climate, and then quantified the spatial variation in ecosystem C storage over time from changes in C turnover time and/or net primary production (NPP). Our results showed that mean ecosystem MTT based on gross primary production (GPP; MTT_{EC_GPP} = C_pool/GPP, 25.0±2.7 years) was shorter than soil MTT (MTT_soil = C_soil/NPP, 35.5±1.2 years) and NPP-based ecosystem MTT (MTT_{EC_NPP} = C_pool/NPP, 50.8±3 years; C_pool and C_soil referred to ecosystem or soil C storage, respectively). On the biome scale, temperature is the best predictor for MTT_EC (R² = 0.77, p<0.001) and MTT_soil (R² = 0.68, p<0.001), while the inclusion of precipitation in the model did not improve the performance of MTT_EC (R² = 0.76, p<0.001). Ecosystem MTT decreased by approximately 4 years from 1901 to 2011 when only temperature was considered, resulting in a large C release from terrestrial ecosystems. The resultant terrestrial C release caused by the decrease in MTT only accounted for about 13.5% of that due to the change in NPP uptake (159.3±1.45 vs. 1215.4±11.0PgC). However, the larger uncertainties in the spatial variation of MTT than temporal changes could lead to a greater impact on ecosystem C storage, which deserves further study in the future.