Journal cover Journal topic
Biogeosciences An interactive open-access journal of the European Geosciences Union
Biogeosciences, 14, 1189-1196, 2017
© Author(s) 2017. This work is distributed
under the Creative Commons Attribution 3.0 License.
Research article
13 Mar 2017
Ice nucleators, bacterial cells and Pseudomonas syringae in precipitation at Jungfraujoch
Emiliano Stopelli1,*, Franz Conen1, Caroline Guilbaud2, Jakob Zopfi3, Christine Alewell1, and Cindy E. Morris2 1Environmental Geosciences, University of Basel, 4056 Basel, Switzerland
2INRA PACA, UR 0407 Plant Pathology Research Unit, 84143 Montfavet, France
3Acquatic and Stable Isotope Biogeochemistry, University of Basel, 4056 Basel, Switzerland
* Invited contribution by Emiliano Stopelli, recipient of the EGU Outstanding Student Poster and PICO (OSPP) Awards 2016.
Abstract. Ice nucleation is a means by which the deposition of an airborne microorganism can be accelerated under favourable meteorological conditions. Analysis of 56 snow samples collected at the high-altitude observatory Jungfraujoch (3580 m a.s.l.) revealed an order-of-magnitude-larger dynamic range of ice-nucleating particles active at −8 °C (INPs−8) compared to the total number of bacterial cells (of which on average 60 % was alive). This indicates a shorter atmospheric residence time for INPs−8. Furthermore, concentrations of INPs−8 decreased much faster, with an increasing fraction of water precipitated from the air mass prior to sampling, than the number of total bacterial cells. Nevertheless, at high wind speeds (> 50 km h−1) the ratio of INPs−8 to total bacterial cells largely remained in a range between 10−2 and 10−3, independent of prior precipitation, likely because of recent injections of particles in regions upwind. Based on our field observations, we conclude that ice nucleators travel shorter legs of distance with the atmospheric water cycle than the majority of bacterial cells. A prominent ice-nucleating bacterium, Pseudomonas syringae, has been previously supposed to benefit from this behaviour as a means to spread via the atmosphere and to colonise new host plants. Therefore, we targeted this bacterium with a selective cultivation approach. P. syringae was successfully isolated for the first time at such an altitude in 3 of 13 samples analysed. Colony-forming units of this species constituted a minor fraction (10−4) of the numbers of INPs−8 in these samples. Overall, our findings expand the geographic range of habitats where this bacterium has been found and corroborate theories on its robustness in the atmosphere and its propensity to spread to colonise new habitats.

Citation: Stopelli, E., Conen, F., Guilbaud, C., Zopfi, J., Alewell, C., and Morris, C. E.: Ice nucleators, bacterial cells and Pseudomonas syringae in precipitation at Jungfraujoch, Biogeosciences, 14, 1189-1196, doi:10.5194/bg-14-1189-2017, 2017.
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Short summary
Based on the analysis of precipitation collected at high altitude, this study provides a relevant advancement in the assessment of the major factors responsible for the abundance and variability of airborne bacterial cells and Pseudomonas syringae in relation to ice nucleators. This is of prime importance to obtain a better understanding of the impact of ice-nucleation-active organisms on the development of precipitation and to determine the dispersal potential of airborne microorganisms.
Based on the analysis of precipitation collected at high altitude, this study provides a...