Biogeosciences, 9, 3891-3899, 2012
https://doi.org/10.5194/bg-9-3891-2012
© Author(s) 2012. This work is distributed under
the Creative Commons Attribution 3.0 License.
Research article
10 Oct 2012
Anaerobic oxidation of methane in grassland soils used for cattle husbandry
A. Bannert1, C. Bogen2, J. Esperschütz2, A. Koubová4,3, F. Buegger5, D. Fischer1, V. Radl1, R. Fuß6, A. Chroňáková4,3, D. Elhottová3, M. Šimek4,3, and M. Schloter1 1Research Unit for Environmental Genomics, Helmholtz Zentrum München – German Research Center for Environmental Health, Ingolstädter Landstraße 1, Neuherberg, 85764, Germany
2Chair of Soil Ecology, Technische Universität München, Ingolstädter Landstraße 1, Neuherberg, 85764, Germany
3Institute of Soil Biology, Biology Centre AS CR, v. v. i., Na Sádkách 7, 37005 České Budějovice, Czech Republic
4Faculty of Science, University of South Bohemia, Branišovská 31, 37005 České Budějovice, Czech Republic
5Institute of Soil Ecology, Helmholtz Zentrum München – German Research Center for Environmental Health, Ingolstädter Landstraße 1, Neuherberg, 85764, Germany
6Institute of Agricultural Climate Research, Johann Heinrich von Thünen Institute, 38116 Braunschweig, Germany
Abstract. While the importance of anaerobic methane oxidation has been reported for marine ecosystems, the role of this process in soils is still questionable. Grasslands used as pastures for cattle overwintering show an increase in anaerobic soil micro-sites caused by animal treading and excrement deposition. Therefore, anaerobic potential methane oxidation activity of severely impacted soil from a cattle winter pasture was investigated in an incubation experiment under anaerobic conditions using 13C-labelled methane. We were able to detect a high microbial activity utilizing CH4 as nutrient source shown by the respiration of 13CO2. Measurements of possible terminal electron acceptors for anaerobic oxidation of methane were carried out. Soil sulfate concentrations were too low to explain the oxidation of the amount of methane added, but enough nitrate and iron(III) were detected. However, only nitrate was consumed during the experiment. 13C-PLFA analyses clearly showed the utilization of CH4 as nutrient source mainly by organisms harbouring 16:1ω7 PLFAs. These lipids were also found as most 13C-enriched fatty acids by Raghoebarsing et al. (2006) after addition of 13CH4 to an enrichment culture coupling denitrification of nitrate to anaerobic oxidation of methane. This might be an indication for anaerobic oxidation of methane by relatives of "Candidatus Methylomirabilis oxyfera" in the investigated grassland soil under the conditions of the incubation experiment.

Citation: Bannert, A., Bogen, C., Esperschütz, J., Koubová, A., Buegger, F., Fischer, D., Radl, V., Fuß, R., Chroňáková, A., Elhottová, D., Šimek, M., and Schloter, M.: Anaerobic oxidation of methane in grassland soils used for cattle husbandry, Biogeosciences, 9, 3891-3899, https://doi.org/10.5194/bg-9-3891-2012, 2012.
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