Abstract. We have studied a suite of 35 sapropel sequences from a transect of four ODP sites across the Eastern Mediterranean to explore for paleoproductivity patterns and provide new insights on ecological changes during their deposition. Paleoproductivity variations were identified using TOC and Ba_bio mass accumulation rates and δ¹⁵N_total and δ¹³C_org values. Elevated Ba/Al and TOC mass accumulation rates record periods of basin-wide amplified productivity. Our data further support that sapropels were formed by cyclic increases in primary production of marine organic matter largely sustained by N-fixing bacteria. This productivity increase was triggered by climate factors leading to increased fluvial discharge and amplified nutrient input that also favored the establishment of N-fixing bacteria. Enhanced productivity led to depletion of deepwater dissolved oxygen and consequently improved organic matter preservation. Primary production was more intense during the middle to Late Pleistocene compared to Pliocene equivalents, coinciding with increasing total sedimentation rates. δ¹⁵N values are dramatically lower in the sapropels than in TOC-poor background sediments, indicating a major contribution from nitrogen-fixing bacteria to the higher productivity during sapropel deposition. Additionally, different degrees of denitrification occurred as a consequence of water column oxygenation which in turns evolved from stagnant anoxic bottom waters during Pliocene sapropels to oxygen depleted and sluggish circulation in late Quaternary layers. These differences between sapropel layers provide new evidences for the general evolution of the Eastern Mediterranean basin during the last 3 Mys in terms of paleoceanographic conditions and the intensity of climate variability leading to sapropel deposition.