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Biogeosciences An interactive open-access journal of the European Geosciences Union
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Volume 3, issue 3
Biogeosciences, 3, 243–249, 2006
https://doi.org/10.5194/bg-3-243-2006
© Author(s) 2006. This work is licensed under
the Creative Commons Attribution-NonCommercial-ShareAlike 2.5 License.

Special issue: Origin and evolution of the biosphere

Biogeosciences, 3, 243–249, 2006
https://doi.org/10.5194/bg-3-243-2006
© Author(s) 2006. This work is licensed under
the Creative Commons Attribution-NonCommercial-ShareAlike 2.5 License.

  20 Jun 2006

20 Jun 2006

The nonenzymatic template-directed ligation of oligonucleotides

A. V. Lutay, E. L. Chernolovskaya, M. A. Zenkova, and V. V. Vlassov A. V. Lutay et al.
  • Institute of Chemical Biology and Fundamental Medicine SB RAS, Lavrentiev Avenue 8, Novosibirsk 630090, Russia

Abstract. The nonenzymatic template-directed ligation of oligonucleotides containing 2', 3'-cyclic phosphate was investigated in the presence of divalent cations. Ligation of the oligonucleotides readily occurred in the presence of Mg2+, Mn2+, Co2+, Zn2+, Pb2+. Efficacy of the metal ion catalysts inversely correlated with pKa values of the metal-bound water molecules. The intermolecular transesterification reaction yielded at least 95metal ion. Relatively high reaction yields (up to 15fragmentation to oligonucleotides with 2',3'-cyclic phosphates, followed by reactions of those oligonucleotides could provide a source of new RNA molecules under prebiotic conditions.

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