Journal cover Journal topic
Biogeosciences An interactive open-access journal of the European Geosciences Union
Journal topic
BG | Volume 16, issue 4
Biogeosciences, 16, 903–916, 2019
https://doi.org/10.5194/bg-16-903-2019
© Author(s) 2019. This work is distributed under
the Creative Commons Attribution 4.0 License.
Biogeosciences, 16, 903–916, 2019
https://doi.org/10.5194/bg-16-903-2019
© Author(s) 2019. This work is distributed under
the Creative Commons Attribution 4.0 License.

Research article 26 Feb 2019

Research article | 26 Feb 2019

Examining the evidence for decoupling between photosynthesis and transpiration during heat extremes

Examining the evidence for decoupling between photosynthesis and transpiration during heat extremes
Martin G. De Kauwe1,2, Belinda E. Medlyn3, Andrew J. Pitman1,2, John E. Drake4, Anna Ukkola5,6, Anne Griebel2, Elise Pendall2, Suzanne Prober7, and Michael Roderick5,6 Martin G. De Kauwe et al.
  • 1ARC Centre of Excellence for Climate Extremes, Sydney, NSW 2052, Australia
  • 2Climate Change Research Centre, University of New South Wales, Sydney, NSW 2052, Australia
  • 3Hawkesbury Institute for the Environment, Western Sydney University, Locked Bag 1797, Penrith, NSW 2751, Australia
  • 4Forest and Natural Resources Management, SUNY-ESF, Syracuse, NY, USA
  • 5ARC Centre of Excellence for Climate Extremes, Canberra, ACT 2601, Australia
  • 6Research School of Earth Sciences, Australian National University, Canberra, ACT 2601, Australia
  • 7CSIRO Ecosystem Sciences, Private Bag 5, Wembley, WA 6913, Australia

Correspondence: Martin G. De Kauwe (mdekauwe@gmail.com)

Abstract
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Recent experimental evidence suggests that during heat extremes, wooded ecosystems may decouple photosynthesis and transpiration, reducing photosynthesis to near zero but increasing transpiration into the boundary layer. This feedback may act to dampen, rather than amplify, heat extremes in wooded ecosystems. We examined eddy covariance databases (OzFlux and FLUXNET2015) to identify whether there was field-based evidence to support these experimental findings. We focused on two types of heat extremes: (i) the 3 days leading up to a temperature extreme, defined as including a daily maximum temperature >37C (similar to the widely used TXx metric), and (ii) heatwaves, defined as 3 or more consecutive days above 35 C. When focusing on (i), we found some evidence of reduced photosynthesis and sustained or increased latent heat fluxes at seven Australian evergreen wooded flux sites. However, when considering the role of vapour pressure deficit and focusing on (ii), we were unable to conclusively disentangle the decoupling between photosynthesis and latent heat flux from the effect of increasing the vapour pressure deficit. Outside of Australia, the Tier-1 FLUXNET2015 database provided limited scope to tackle this issue as it does not sample sufficient high temperature events with which to probe the physiological response of trees to extreme heat. Thus, further work is required to determine whether this photosynthetic decoupling occurs widely, ideally by matching experimental species with those found at eddy covariance tower sites. Such measurements would allow this decoupling mechanism to be probed experimentally and at the ecosystem scale. Transpiration during heatwaves remains a key issue to resolve, as no land surface model includes a decoupling mechanism, and any potential dampening of the land–atmosphere amplification is thus not included in climate model projections.

Please read the corrigendum first before continuing.
1 Introduction
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In response to a warming climate, heatwaves have increased in frequency, magnitude and duration (Alexander et al., 2006; Perkins et al., 2012). Coupled climate models from the Coupled Model Intercomparison Project (CMIP5) project a marked increase in the frequency and severity of these heat extremes (Coumou and Robinson, 2013; Sillmann et al., 2013), highlighting the urgent need to understand the underlying driving mechanisms. Whilst heatwaves are commonly associated with large-scale, high-pressure synoptic systems (anticyclones) (Perkins, 2015), there is increasing evidence of the role of the land surface in the amplification of heat extremes (Fischer et al., 2007; Teuling et al., 2010; Miralles et al., 2012; Kala et al., 2016; Donat et al., 2017). This land–atmosphere feedback is driven by drying soils and an increase in the sensible heat flux which further warms the boundary layer (Lorenz et al., 2010; Seneviratne et al., 2006). The combination of heat advection and heat storage in the boundary layer is recycled back to the surface over successive days and can lead to increasingly intense heatwaves, including “mega-heatwaves” (Miralles et al., 2014).

A number of studies have highlighted the contrasting functional traits of grasslands and forests as important controls on the role of the land surface in the amplification of heatwaves (Teuling et al., 2010; van Heerwaarden and Teuling, 2014). Grasses often have shallow root profiles, meaning that a relatively small reduction in soil moisture can stress a grassland, resulting in decreased transpiration (either directly via reduced stomatal conductance and/or indirectly via reduced leaf area), leading to a repartitioning of the available (radiant) energy towards sensible heat. Heatwaves also affect forests, but the deeper root profiles that characterize forests may make surface drying less likely to influence the surface energy balance. However, whilst this slower soil water depletion may buffer the transition to increased sensible heat flux, ultimately the decline in soil moisture may still result in heatwave intensification during prolonged dry spells (Teuling et al., 2010).

On the other hand, recent experimental evidence has highlighted a previously overlooked vegetation–atmosphere feedback that may in fact dampen, rather than amplify, heat extremes. A number of heatwave experiments carried out in well-watered, potted plants, have suggested that during temperature extremes, photosynthesis and stomatal conductance (gs) become decoupled, such that photosynthesis is reduced to near zero but transpiration is maintained (Ameye et al., 2012; von Caemmerer and Evans, 2015; Urban et al., 2017). For example, in a growth chamber study, Urban et al. (2017) found that gs increased with rising temperature despite photosynthetic activity shutting down for both Pinus taeda and Populus deltoides×nigra. This result was also confirmed in a field-based whole-tree chamber study by Drake et al. (2018), who reported that transpiration was increased and decoupled from photosynthesis in 6 m tall Eucalyptus parramattensis trees during an imposed heatwave of 4 consecutive days with temperatures exceeding 43 C. Crucially, in the Drake et al. (2018) study, the plants were not well-watered. Instead, these trees had been subject to an imposed 1-month drought prior to the experiment to reduce soil water stores. Evidence that transpiration increases during a heatwave, resulting in a cooler canopy temperature, would be consistent with an active mechanism (Trewavas et al., 2009) by trees to cool their canopies. Such a response to heat extremes would increase the latent heat flux into the boundary layer and have two major negative feedbacks on heat extremes: first, the increase in latent heat flux would be at the cost of the sensible heat flux and a reduction in sensible heat flux would potentially reduce any land amplification on heatwaves over forested regions. Second, by moistening the boundary layer, the chance of clouds being formed would increase, leading to a decrease in solar radiation at the surface and a consequent cooling effect.

In climate models, including CMIP5 models, the land surface is represented by modules that assume the photosynthesis and gs (and consequently transpiration) are inherently coupled (De Kauwe et al., 2013). At high temperatures, models assume that photosynthesis is reduced due to (i) the direct impairment of the photosynthetic biochemistry; (ii) increased respiration; and (iii) reduced gs due to the associated high vapour pressure deficit. Finding additional evidence of a decoupling between photosynthesis and gs at high temperatures would therefore require revisiting existing assumptions embedded in all climate models and have important implications for model-based assessments of the role of the land surface in the amplification of heat extremes.

Here, we hypothesized that evidence of decoupling would present itself as a reduction in gross primary productivity (GPP) and an increase in latent heat flux (LE) as air temperatures increased. It is important to clarify that decoupling does not mean that gs will increase as GPP declines, only that it will decline less strongly than current theory would predict if photosynthesis and gs remained coupled. As temperature increases, vapour pressure deficit (D) also increases, which will drive an increase in LE unless there is stomatal closure, but this effect is unrelated to the decoupling mechanism we seek to find. To disentangle the potentially contributing role of D, we also explored these data based on the theoretical expectation (Lloyd et al., 1991; Medlyn et al., 2011; Zhou et al., 2014) that transpiration (E) is approximately proportional to GPP ×D0.5 (g C kPa0.5 m−2 d−1; Eq. 7). This expectation is based the idea of optimal stomatal behaviour proposed by Cowan and Farquhar (1977) that stomata should be regulated so as to maximize photosynthetic carbon gain for a given amount of transpiration. Medlyn et al. (2011) derived the optimal stomatal behaviour as

(1)Gs=1.61+g1DACa,

where Gs is canopy stomatal conductance to CO2 (mol m−2 s−1), A is the net assimilation rate (µmol m−2 s−1), Ca is the ambient atmospheric CO2 concentration (µmol mol−1), D is the vapour pressure deficit (kPa), the parameter g1 (kPa0.5) is a fitted parameter representing the sensitivity of the conductance to the assimilation rate and the factor 1.6 is the ratio of diffusivity of water to CO2 in air. Assuming that transpiration is largely controlled by conductance, this relationship can be rearranged to show that water use efficiency (AE) is approximately proportional to 1/D. This dependence has been remarked by many authors (e.g. Lloyd, 1991; Katul et al., 2009). Based on this dependence, Zhou et al. (2014, 2015) proposed an “underlying water use efficiency” (uWUE) for eddy covariance data:

(2)uWUEGPPDE.

Zhou et al. (2014) argued that the D0.5 term provided a better linear relationship between GPP and E. Thus, to probe the effect of D, we focused on heatwaves (i.e. approach 2) and plotted LE expressed as evapotranspiration (mm day−1), as a function of GPP ×D0.5. We note for the interested reader tracing the development of the optimal stomatal theory through the cited publications that Eq. (7) in Medlyn et al. (2011) is missing a pressure (P) term in the numerator (under the square root sign), which ensures the equation is dimensionally correct. However, the equation is not used in any further derivation in Medlyn et el. (2011) and so the missing term does not have any impact on the theory presented in the rest of that paper.

In this paper we therefore explore eddy covariance measurements to examine whether there is widespread field-based evidence that during heat extremes, trees decouple photosynthesis and gs, leading to increased transpiration. In contrast to previous experimental studies (e.g. Urban et al., 2017), our focus is on the ecosystem scale, and so we analysed the photosynthetic decoupling between photosynthesis and transpiration using theory derived from optimal stomatal behaviour (Lloyd, 1991; Medlyn et al., 2017; Zhou et al., 2014). We chose to focus on wooded ecosystems as the capacity to maintain transpiration throughout a heat extreme most likely requires deep soil water access and is in line with previous experimental evidence from trees (Drake et al., 2018; Urban et al., 2017).

2 Materials and methods
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2.1 Evidence of photosynthesis–transpiration decoupling

A number of experimental studies reporting photosynthetic decoupling have focused on the coupling between A and gs (Weston and Bauerle, 2007; Ameye et al., 2012; von Caemmerer and Evans, 2015), as opposed to A and E (Drake et al., 2018). At the ecosystem scale (eddy covariance), coincident measurements of Gs and LE (or transpiration) are rarely available. Whilst it is possible to estimate the canopy Gs by inverting the Penman–Monteith equation using measured LE, such an approach necessitates additional assumptions related to the canopy boundary layer conductance (Jarvis and McNaughton, 1986; De Kauwe et al., 2017), the canopy net radiation and the ground heat flux (Medlyn et al., 2017). Here we avoid these assumptions by focusing our analysis on the measured LE flux, as opposed to an estimate of the canopy Gs.

A range of definitions currently exist to identify an extreme temperature event (see Perkins et al., 2015, for a review). Most of these are defined from the context of the climate and may not reflect the physiological adaptations of the vegetation. Given this lack of a single unifying definition, we tested two approaches on the eddy covariance measurements: (1) the change in GPP and latent heat flux during the 4 days leading up to and including a temperature extreme, where a temperature extreme was defined as being a day when the daily maximum temperature exceeded 37 C, and (2) the change in GPP and latent heat flux during a heatwave, defined as at least 3 consecutive days where the maximum daily temperature exceeded 35 C. The first approach can be viewed as analogous to the behaviour leading up to the hottest day of the year (commonly defined as TXx; Klein Tank et al., 2009) and the imposed lower boundary of 37 C similar to selecting a number of “hot” days by using a percentile from the TXx but defined from a more physiological standpoint. This temperature threshold was selected to ensure the events were hot enough to stress the vegetation (Curtis et al., 2016; O'Sullivan et al., 2017; Zhu et al., 2018). For the Australian sites, 37 C was consistent with a site's climate of origin + threshold (mean summer maximum temperature; Tmax+10C) (Drake et al., 2017).

For each of these events we recorded the maximum daytime temperature, the mean daytime (06:00–20:00 LT) LE and the daytime summed GPP. Although we chose to compare mean daytime LE and the summed daytime GPP with the maximum daytime temperature, there are of course alternative analysis approaches. We chose our approach as an appropriate trade-off in time resolution that facilitated us to consider several heat-extreme events, across multiple sites. This allowed us to see the broader patterns of behaviour at the ecosystem scale. Had we considered analysing the raw 30 min data for example, we felt that interpretation of the underlying behaviour would have been made considerably more difficult due to the increased time frequency and inherent noise in these data. A further alternative analysis approach would have been to compare the maximum or daily mean temperature with the midday GPP and LE fluxes; however, we felt such an approach could miss interesting morning and afternoon responses which may result directly from the temperature extremes but not be present in the midday observation.

To test for evidence of photosynthetic decoupling in the ecosystem-scale fluxes we fitted a linear regression to the fluxes from each event leading up to a day where the maximum temperature exceeded 37 C (i.e. approach 1 above), showing events where the fitted slope was negative for GPP and positive for LE. We do not necessarily expect the response of GPP or LE to be linear with respect to increasing temperature, but selecting events based on their fitted (positive/negative) slopes allows us to identify patterns in the data. We do not seek to draw inferences from the fitted slope being significant or not, given the small number of samples (n=4) in each event. We simply use this distinction to identify stronger positive or negative trends in these data. To disentangle the potentially contributing role of D, we also explored flux behaviour based on the theoretical expectation (Lloyd et al., 1991; Medlyn et al., 2011; Zhou et al., 2014) that E is approximately proportional to GPP ×D0.5 (g C kPa0.5 m−2 d−1). To address this issue, we focused on heatwave events (i.e. approach 2 above).

2.2 Flux data

Half-hourly eddy covariance measurements of the exchange of carbon dioxide, energy, and water vapour were obtained from OzFlux (http://www.ozflux.org.au/, last access: 26 April 2017) and FLUXNET2015 (http://fluxnet.fluxdata.org/data/fluxnet2015-dataset, last access: 26 April 2017) and releases. We confined our FLUXNET2015 analysis to sites classified as wooded according to the International Geosphere–Biosphere Programme, namely evergreen needleleaf forest, evergreen broadleaved forest and deciduous broadleaved forest (albeit noting that these names have an inherently Northern Hemisphere bias and would be better classified as evergreen coniferous, evergreen angiosperm and deciduous angiosperm forest, respectively). We excluded sites classified as savanna due to the associated complication of needing to attribute the total transpiration flux to grasses and trees; however, we do acknowledge that many of the Australian sites are also relatively open (see screening step below). We also excluded sites classified as mixed forest from our analysis or those that did not meet our physiological threshold of a daily maximum temperature that exceeded 37 C. We also excluded sites that experienced burning. A total of nine sites met these criteria in the Tier 1 (freely available) FLUXNET 2015 database. FLUXNET data were preprocessed using the FluxnetLSM R package (Ukkola et al., 2017). For OzFlux, we used Level 6 gap-filled data following Isaac et al. (2017). These data were then screened to only keep measured and good-quality gap-filled data. Events were ignored if a rainfall event greater than 0.5 mm day−1 was observed during or in the 2 days prior to a heat event in the eddy covariance data (Dekker et al., 2001, 2016; Law et al., 2002; Groenendijk et al., 2011; Keenan et al., 2013; De Kauwe et al., 2017; Knauer et al., 2017; Medlyn et al., 2017) as this could bias the LE flux by leading to an increase in LE not associated with the mechanism we wished to identify (i.e. due to soil/canopy evaporation). Knauer et al. (2017) is the only study to have explicitly tested the impact of assuming that 2 days following a rainfall event, the LE flux is dominated by transpiration. Across six FLUXNET sites, they found between a 9 % and 19 % change in estimates of the slope parameter of the optimal stomatal parameter (g1; Medlyn et al., 2017) with increasing time since the last rainfall event beyond 48 h (out to 240 h). However, their analysis did not account for the potential confounding effect that as they screened a greater number of hours following rainfall, the number of samples used to estimate the g1 parameter was also reduced, which would increase the error in estimates of the model parameter. Given both the high temperatures considered in our analysis framework and the length of the period after screening for rain (at least 3 days), we would expect the impact of soil evaporation to be a minor consideration.

2.3 Accumulated heat stress

To characterize a measure of the annual heat accumulated stress experienced by the vegetation, we calculated the average number of growing degree days above our upper threshold of 37 C year−1 (GDD37). We used surface air temperature from the 6-hourly, reanalysis by the Global Soil Wetness Project Phase 3 (GSWP3; http://hydro.iis.u-tokyo.ac.jp/GSWP3, last acccess: 21 January 2019, and Dirmeyer et al., 2006) dataset during the period of 1970–2015 at a 0.5 spatial resolution. We opted to use this coarser dataset to estimate GDD37 rather than the observed flux record due to the longer temporal record, which is likely to be more reflective of longer-term conditions.

2.4 Analysis code

All analysis code is freely available from https://github.com/mdekauwe/heat_extremes_decoupling (last access: 26 April 2017).

Table 1OzFlux and FLUXNET2015 site information. GDD37 is the average number of growing degree days above our threshold of 37 C. Plant functional types (PFTs) were ENF (evergreen needleleaf forest), EBF (evergreen broadleaved forest) and DBF (deciduous broadleaved forest). Note the FLUXNET sites Castel d'Asso 1 and 3 and Roccarespampani 1 and 2 have been combined in the table.

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3 Results
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We first focus on the Australian sites as these experienced more temperature extremes due to the warm climate. We found significant evidence of thermal heat stress (Table 1), with 85.8 GDD37 at Alice Springs, 85.1 GDD37 at Great Western Woodlands, 68.3 at Calperum, 31.7 at Gingin, 13.5 at Cumberland Plains, 13.4 at Whroo and 3.1 at Wombat.

Figure 1 shows a consistent reduction in the flux-derived GPP with increasing daily maximum temperature for each of the events (4-day events, where the maximum temperature > 37 C). We emphasize (see Sect. 2) that one should only interpret differences between significant negative slopes (dark blue lines) and negative slopes (dark green lines) as indicative of (possibly) stronger or more consistent reductions in GPP as a function of temperature. This reduction in GPP follows theory related to biochemical, respiratory and stomatal drivers (Lin et al., 2012). With the exception of the Whroo site, GPP was reduced to close to zero at temperatures greater than 40 C. Figure S1 in the Supplement shows the limited occurrences where the fitted slopes indicated a positive (or arguably flat) response with increasing temperature.

Evidence for the hypothesized decoupling between photosynthesis and gs, which would lead to an increase in LE with temperature (but a concomitant decline in GPP, Fig. 1), is shown in Fig. 2. Despite variability in the measured data, at each of the seven sites, LE is found to increase or be sustained as the temperature increases in the lead-up to the maximum temperature of each heat event. This increase is steepest at the Wombat State Forest site but is based on only one GDD37 event (Table S1). At the other sites, the magnitude of the increase is smaller. However, it is clear that the LE flux is not reduced in line with GPP (Fig. 1) and instead remains sustained with temperature throughout the extreme events. Figure S2 shows the occurrences where the fitted slopes indicated a negative response with increasing temperature. In many cases these events were broadly flat in response to increasing temperature, again indicating a sustained LE flux. Taken together, Figs. 1, 2 and S2 provide consistent evidence of a decoupling between photosynthesis and transpiration during significant heat extremes across a range of Australian wooded ecosystems.

https://www.biogeosciences.net/16/903/2019/bg-16-903-2019-f01

Figure 1Evolution of GPP in the 3 days prior to and including a hot temperature extreme (daily maximum temperature exceeded 37 C). Dark blue lines represent events in which a fitted linear regression indicated a significant negative slope, whilst dark green lines represent events where the fitted slope was negative but not significant. Note in both cases that we are not showing the fitted slopes, but we are simply using this approach to identify stronger positive or negative trends in these data (see Sect. 2). Events where the fitted slope was positive are shown in Fig. S1.

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We now seek to explore the strength of this apparent decoupling in more detail by looking at the ratio of positive to negative fitted slopes shown in Figs. 1, 2, S1 and S2. Figures 3 and 4 shows the distribution of fitted positive and negative slopes as a function of temperature across the Australian sites for GPP and LE, respectively. Whilst the fitted slopes for GPP are predominately negative (Fig. 3), there does not appear to be a consistent pattern in the frequency of positive vs. negative fitted LE slopes, with some sites having more positive slopes (e.g. Gingin, Great Western Woodlands) and some registering more negative slopes (Calperum, Whroo), while others are about even (Alice Springs, Cumberland Plains) (Fig. 4). This result is not surprising given our hypothesis that significant transpiration during a heatwave is dependent upon the available supply of soil moisture. As soil water supply becomes limiting, we would expect to find more frequent negative slopes. Consistent with this link to soil moisture, there is a small drop in the proportion of positive slopes (i.e. increased LE) towards the end of summer, which is coincident with an increase in the frequency of negative slopes (Fig. S3).

Evidence for an increase in LE with temperature and for photosynthetic decoupling during heat extremes was much weaker across the seven FLUXNET2015 sites (excluding Australian sites; Figs. S4 and S5) that exceeded our 37 C threshold. The number of concomitant negative GPP slopes (Fig. S4) and positive LE slopes (Fig. S5) was noticeably lower when compared to Australian sites, making it harder to draw clear inferences. On the one hand, the weaker evidence from across the larger FLUXNET2015 dataset may point to this decoupling behaviour being species or climatic zone specific (i.e. located in very hot environments). However, we would caution against that interpretation as it is as likely to also point to the lack of representation of FLUXNET sites in regions, other than Australia, that experience very hot temperature extremes (e.g. the average GDD37 for the non-Australian sites was >1 at only two sites; Table 1). Given the limited signal in the results obtained from FLUXNET2015 sites, we continue to focus our analysis on Australian sites. However, given the extremely hot summer experienced across Europe in 2018, future studies may wish to revisit this analysis as these updated flux data become available.

https://www.biogeosciences.net/16/903/2019/bg-16-903-2019-f02

Figure 2Evolution of LE in the 3 days prior to and including a hot temperature extreme (daily maximum temperature exceeded 37 C). Dark blue lines represent events in which a fitted linear regression indicated a significant positive slope, whilst dark green lines represent events where the fitted slope was positive but not significant. Note in both cases that we are not showing the fitted slopes, but we are simply using this approach to identify stronger positive or negative trends in these data (see Sect. 2). Events where the fitted slope was negative are shown in Fig. S2.

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Increasing temperature also usually leads to increasing D and as a result, even with perfect coupling between photosynthesis and gs, we would still expect to see transpiration changing as a function of GPP ×D0.5. Figure 5 shows this relationship for consecutive heatwave and non-heatwave days (note Wombat State Forest was excluded from this analysis as there were insufficient consecutive days > 35 C). If the change in transpiration was being driven by a decoupling of gs from the response of photosynthesis, we might expect to see increasing transpiration for a given GPP ×D0.5, i.e. a spread in points vertically for heatwave days. If the change was being driven by increasing water use efficiency, we might expect to see an increased GPP ×D0.5 for a given unit of transpiration, i.e. a spread horizontally for heatwave days. Across the sites there was no clear difference in the behaviour for heatwave vs. non-heatwave days. At Calperum, Cumberland Plains and Whroo, the relationship between GPP ×D0.5 and transpiration was fairly constant, whereas at Great Western Woodlands, transpiration for a given GPP ×D0.5 on heatwave days was slightly higher than on non-heatwave days and at Alice Springs and Gingin, slightly lower. At Alice Springs and Gingin, this seems to fit with our expectation of increasing D driving increasing water use efficiency, i.e. not the decoupling mechanism. At Great Western Woodlands, there is some indication that the data spread vertically, which may be consistent with our expectation outlined for decoupling, but the pattern is not conclusive.

4 Discussion
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Recent experimental studies (Drake et al., 2018; Urban et al., 2017) have identified that at very high temperatures (> 40 C), plants decouple photosynthesis and gs and instead increase transpiration in an apparent active process to cool their canopies. Our results from across seven wooded ecosystems located in Australia were inconclusive. We found some indication (Figs. 1–4) that LE was increased or sustained as GPP decreased when exploring the behaviour in the lead-up to the hottest days of the year. However, when we focused on heatwave events (i.e. consecutive days >35C; Fig. 5) and considered the role of D, i.e. as a driver of increased LE, rather than a photosynthetic decoupling that would increase the transpiration flux to cool the canopy (i.e. in response to leaf temperature), we found little clear support for photosynthetic decoupling.

https://www.biogeosciences.net/16/903/2019/bg-16-903-2019-f03

Figure 3Probability density and histogram showing the distribution of fitted positive and negative GPP slopes across the OzFlux sites. The dark blue curve shows the fitted kernel density estimate (KDE) and the vertical blue lines along the x axis are “rugs”, which represent the individual occurrence of fitted slopes. Data from Wombat State Forest have been omitted from the graph as there was only one slope. Note that the sum of the bars can exceed 1 as the normalization ensures that the sum of the bar heights multiplied by the bar widths equals 1, which allows the normalized histogram to be compared to the KDE, which is normalized so that the area under the curve equals 1.

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Figure 4Probability density and histogram showing the distribution of fitted positive and negative LE slopes across the OzFlux sites. The dark blue curve shows the fitted kernel density estimate (KDE) and the vertical blue lines along the x axis are “rugs”, which represent the individual occurrence of fitted slopes. Data from Wombat State Forest have been omitted from the graph as there was only one slope. Note that the sum of the bars can exceed 1 as the normalization ensures that the sum of the bar heights multiplied by the bar widths equals 1, which allows the normalized histogram to be compared to the KDE, which is normalized so that the area under the curve equals 1.

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As the background climate warms with associated changes in the intensity and frequency of heat extremes, there is a growing interest in the degree to which leaf temperature affects, and is affected by, the physiological response of plants. The potential for plants to use a photosynthetic-decoupling mechanism to apparently regulate leaf temperatures is one emerging aspect of this interplay between plant physiology and temperature. Other studies are currently questioning other widely accepted notions about stomatal regulation. For example, Cernusak et al. (2018) recently examined the near-universal assumption that vapour pressure inside a leaf remains saturated in all conditions. They found in two conifer species that, under moderate to high D, this assumption was invalid, leading to a bias in the calculated gs. Similarly, Kowalski et al. (2017) have recently questioned the paradigm that all transport through stomata is diffusive, instead invoking the concept of non-diffusive stomatal jets. However, neither of these papers provides a mechanism by which stomatal closure would be decoupled from photosynthesis. Further plant physiological studies are required to identify this mechanism.

4.1 Why did we not find supporting evidence for ecosystem-scale photosynthetic decoupling?

One interpretation of the apparent contradictions between the findings of previous studies and our lack of conclusive evidence at the ecosystem scale, may simply relate to the interpretation scale. At the leaf level, plants usually reduce gs exponentially with increasing D (Oren et al., 1999). However, at high temperatures and with the associated high D, the increased atmospheric demand for water may drive an increase in the transpiration rate. In well-controlled environments, it may be possible to separate the direct response to temperature from that of D, but as our analysis shows, this is more complicated with ecosystem-scale data.

https://www.biogeosciences.net/16/903/2019/bg-16-903-2019-f05

Figure 5E as a function of GPP ×D0.5 on heatwave and non-heatwave days. The solid lines are smoothed time series using a generalized additive model (with a 95 % confidence intervals). Note that data from Wombat State Forest have also been omitted from the graph as there were insufficient heat wave events and the generalized additive model was not fit to the heatwave days at Cumberland Plains due to the limited data.

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The recent work by Drake et al. (2018) demonstrated clear evidence of photosynthetic decoupling at the canopy scale using a series of whole-tree chambers, which would suggest that this mechanism is unlikely to simply be scale dependent. However, to infer the photosynthetic decoupling, Drake et al. (2018) demonstrated that the observed decline in gs (and so transpiration) was weaker than predicted by a coupled leaf Ags model, which was specifically calibrated to the experimental data. This approach is not viable across multiple sites as it necessitates detailed site measurements for calibrations that are often prohibited by the tall canopy height of mature stands. Applying such a coupled model (e.g. a land surface model) to these site data simply demonstrates that the model is unable to capture the observed site responses (not shown). As a result, we could not reliably infer that the divergence from model behaviour points to evidence of photosynthetic-decoupling, as opposed to, for example, poor parameterization associated with stand-level attributes such as leaf area index or root-zone soil moisture.

A number of the previous studies that showed photosynthetic decoupling experimentally were carried out on well-watered plants (Ameye et al., 2012; Urban et al., 2017). Thus, one interpretation of our results is simply that root-zone soil moisture was limiting any photosynthetic decoupling. In Drake et al. (2017), irrigation of the whole-tree chambers was withheld for the month prior to the heatwave experiment; thus, a more nuanced interpretation may be that a photosynthetic-decoupling mechanism requires access to soil moisture from deeper in the profile (perhaps associated with access to groundwater). Without data throughout the root-zone profile across the flux sites, we cannot rule out this explanation. Our results did show tentative evidence consistent with this explanation; we found a small decrease in the number of positive slopes (i.e. increased LE) towards the end of the summer (Fig. S3), which may reflect reduced soil water availability to sustain transpiration. Using sap flow data, Tatarinov et al. (2015) found a  60 % decrease in canopy conductance, an approximate halving of daytime GPP, but little change in ET during spring heat waves (“hamsin”) in a 50-year-old Aleppo pine forest located at the edge of the Negev desert. The observed responses during these Mediterranean heat extremes are consistent with a photosynthetic decoupling, although in their study, we note that the authors attributed these differences in behaviour to the relative influence of D and soil moisture availability.

One could ask whether our analysis considered hot enough temperatures (> 37 C) to trigger a photosynthetic-decoupling mechanism. For example, during an imposed heatwave, Ameye et al. (2012) probed the decoupling mechanism at daily maximum temperatures between 47 and 53 C. Similarly, Zhu et al. (2018) found that most of the 62 species sampled across Australia exhibited maximum critical temperatures near 50 C. However, the temperature optima for leaf and canopy photosynthesis in Eucalypts in southern Australia are well below 30 C (see Duursma et al., 2014; Drake et al., 2016; Kumarathunge et al., 2019), suggesting that days above 37 C should induce a decline in GPP. Our analysis also included events with daily maximum temperatures of greater than 40 C and consecutive heatwave days > 35 C. Therefore, we would argue that insufficiently high temperatures are unlikely to explain why we did not see clear evidence when looking at eddy covariance data.

Our approach relies on GPP which is not directly observed but is instead modelled using assumptions related to the extrapolation of night-time respiration and measured net ecosystem exchange. It is debatable whether these assumptions hold at very high temperatures, and examining these modelled GPP estimates at high temperatures warrants further investigation, particular as researchers leverage these data to explore the responses of the vegetation to temperature extremes. Eddy covariance data are also known to have issues closing the energy balance (Wilson et al., 2002; Foken, 2008; Hendricks-Franssen et al., 2010; Eder et al., 2015), which may introduce errors into the LE flux (see Wohlfahrt et al., 2009, for a detailed discussion). For the seven Australian flux sites that make up the majority of our analysis, we calculated the ratio of the sum of latent and sensible heat fluxes to the sum of the net radiation and ground heat flux, finding on average a  17 % imbalance in the ratio (range 7 %–30 %). Importantly however, we did not find any difference in this imbalance in heatwave vs. non-heatwave days. This is in line with other studies. Despite these limitations, FLUXNET eddy covariance flux measurements still present our best ecosystem-scale estimates of vegetation responses to heat extremes and have been widely analysed to address these types of questions (Ciais et al., 2005; Teuling et al., 2010; Wolf et al., 2013; von Buttlar et al., 2018; Flach et al., 2018).

Our analysis is also limited by the number of extreme events recorded in the existing record, and the clear bias in these data towards Australian sites is due to the lack of representation of sites within the FLUXNET data collection that sample locations in extreme environments outside of Australia. In our analysis we focused on hot days and heatwaves with a very hot temperature range, i.e. consecutive days > 35 C; hence, a fair criticism of our approach is that a lower threshold might be also relevant for different environments and species. Any choice of threshold is arguably arbitrary; we chose ours to ensure we were focusing on the vegetation response to a threshold that would lead to a degree of physiological limitation and is in line with studies that suggest this occur at temperatures above our chosen thresholds (Curtis et al., 2016; O'Sullivan et al., 2017; Zhu et al., 2018).

Although Drake et al. (2018) did not find evidence of increased litterfall during their heatwave experiment, it is of course possible that across the FLUXNET sites we considered, there was some reduction in leaf area in response to high temperature extremes. However, any leaf area reduction would reduce both transpiration and photosynthesis, and thus we think it is unlikely to affect ecosystem-scale estimates of a photosynthetic decoupling. Nevertheless, future flux-based experiments may consider also using leaf litter traps at sites to allow researchers to separate out this effect and confirm this assumption.

Finally, throughout our paper we have treated the measured LE flux as interchangeable with the transpiration flux (i.e. ignoring any potential role of soil and or canopy evaporation – see Sect. 2.2). Strictly, if soil and/or canopy evaporation fluxes were not zero, the signal that we have analysed would contain a contribution that is not directly under the plant's control and so could not be affected (directly) by any photosynthetic decoupling. Whilst we cannot rule out such a contribution, we expect it to be unlikely to be a significant factor at play in our results. Screening the eddy covariance time series for the 2 days following observed rain events follows a widely used strategy in eddy covariance studies (Dekker et al., 2001, 2016; Law et al., 2002; Groenendijk et al., 2011; Keenan et al., 2013; De Kauwe et al., 2017; Knauer et al., 2017; Medlyn et al., 2017). Moreover, our analysis also considers heat extremes that last for at least 3 further days. Thus, after 5 days (2 days prior to event must also have been rain free), in temperatures exceeding 30 C, we think it likely that the latent heat flux will be dominated by transpiration.

4.2 Implications for models

The potential implications for modelling studies that focus on heat extremes are clear, particularly for studies in Australia. None of the current generation of land surface models have the capacity to decouple transpiration from the down-regulation of photosynthesis with increasing temperature. Instead models assume that photosynthesis and gs (and consequently transpiration) remain coupled at all times. As a result, climate models may underestimate the capacity of the vegetation to dampen heat extremes in simulations for Australia. This is also true of more sophisticated plant hydraulic models (Williams et al., 2001) and profit-maximization approaches (Wolf et al., 2016; Sperry et al., 2017) that hypothesize that the cost of water is not fixed in time but instead increases with increasing water stress. For these latter approaches to account for a photosynthetic decoupling, they would need to prioritize maintaining an optimum canopy temperature above a net carbon gain. However, mechanisms to capture this within models should likely wait for further supporting evidence of photosynthetic decoupling.

5 Conclusion
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A number of recent experimental studies have highlighted that during heat extremes, plants may decouple photosynthesis and transpiration, reducing photosynthesis to near zero but increasing transpiration into the boundary layer. In this study we used eddy covariance measurements to examine the evidence for a photosynthetic decoupling in wooded ecosystems at the ecosystem scale during heat extremes. When focusing on the 3 days leading up to a temperature extreme (a daily maximum > 37 C), we found some evidence of reduced photosynthesis and sustained or increased latent heat fluxes at seven Australian evergreen wooded flux sites. However, when considering the role of vapour pressure deficit, we were unable to conclusively disentangle photosynthetic decoupling from the effect of increase in transpiration due to increasing vapour pressure deficit during heatwaves (3 or more consecutive days above 35 C). However, it would be premature to interpret our results as evidence that such a mechanism does not scale from the leaf to the ecosystem. Instead, understanding the response of transpiration during heatwaves remains an important issue to resolve. It is clear that further experimental results will be required to resolve this issue, and these studies will need to be able to more clearly separate the decoupling mechanism from the response to D and other potential factors (see Sect. 4.1). To make progress on this photosynthetic-decoupling issue will likely require concurrent leaf-level gas-exchange measurements (photosynthesis and gs) as well as canopy or ecosystem-scale transpiration. To date, most of our insight has been limited to the leaf scale (Ameye et al., 2012; von Caemmerer and Evans, 2015; Urban et al., 2017) or a single canopy-scale study situated in whole-tree chambers (Drake et al., 2018). To bridge this gap in our knowledge, it would be desirable to align future experiments with measurements taken at eddy covariance sites (i.e. by using matching species) to allow us to more easily test whether this mechanism scales to the ecosystem.

Code and data availability
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Code and data availability. 

All code is freely available from https://github.com/mdekauwe/heat_extremes_decoupling (last access: 12 February 2019, De Kauwe, 2019).

All Eddy covariance data are available from http://www.ozflux.org.au/ (last access: 26 April 2017) and http://fluxnet.fluxdata.org/data/fluxnet2015-dataset (last access: 26 April 2017).

Supplement
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Supplement. 

The supplement related to this article is available online at: https://doi.org/10.5194/bg-16-903-2019-supplement.

Author contributions
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Author contributions. 

MDK conceived and designed the study based on discussions involving MDK, BEM and JED. MDK wrote the code and analysed the results. AU assembled and processed the eddy covariance data. All authors contributed to the writing of the paper.

Competing interests
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Competing interests. 

The authors declare that they have no conflict of interest.

Acknowledgements
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Acknowledgements. 

Martin G. De Kauwe acknowledges support from the Australian Research Council Centre of Excellence for Climate Extremes (CE170100023). This work used eddy covariance data acquired by the FLUXNET community and in particular by the following networks: AmeriFlux (U.S. Department of Energy, Biological and Environmental Research, Terrestrial Carbon Program (DE-FG02-04ER63917 and DE-FG02-04ER63911)), AfriFlux, AsiaFlux, CarboAfrica, CarboEuropeIP, CarboItaly, CarboMont, ChinaFlux, Fluxnet-Canada (supported by CFCAS, NSERC, BIOCAP, Environment Canada and NRCan), GreenGrass, KoFlux, LBA, NECC, OzFlux, TCOS-Siberia and USCCC. We acknowledge the financial support to the eddy covariance data harmonization provided by CarboEuropeIP, FAO-GTOS-TCO, iLEAPS, the Max Planck Institute for Biogeochemistry, the National Science Foundation, the University of Tuscia, Université Laval and Environment Canada, and the US Department of Energy; we also acknowledge the database development and technical support from Berkeley Water Center, Lawrence Berkeley National Laboratory, Microsoft Research eScience, Oak Ridge National Laboratory, the University of California and the University of Virginia.

Edited by: Dan Yakir
Reviewed by: Josef Urban, Andrew Kowalski, and Ryan Teuling

References
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Alexander, L., Zhang, X., Peterson, T., Caesar, J., Gleason, B., Klein Tank, A., Haylock, M., Collins, D., Trewin, B., Rahimzadeh, F., Tagipour, A., Rupa Kumar, K., Revadekar, J., Griffiths, G., Vincent, L., Stephenson, D. B., Burn, J., Aguilar, E., Brunet, M., Taylor, M., New, M., Zhai, P., Rusticucci, M., and Vazquez-Aguirre, J. L.: Global observed changes in daily climate extremes of temperature and precipitation, J. Geophys. Res.-Atmos., 111, D05109, https://doi.org/10.1029/2005JD006290, 2006. 

Ameye, M., Wertin, T. M., Bauweraerts, I., McGuire, M. A., Teskey, R. O., and Steppe, K.: The effect of induced heat waves on Pinus taeda and Quercus rubra seedlings in ambient and elevated CO2 atmospheres, New Phytol., 196, 448–461, 2012. 

Berbigier, P., Bonnefond, J.-M., and Mellmann, P.: CO2 and water vapour fluxes for 2 years above Euroflux forest site, Agricult. Forest Meteorol., 108, 183–197, 2001. 

Cernusak, L. A., Ubierna, N., Jenkins, M. W., Garrity, S. R., Rahn, T., Powers, H. H., Hanson, D. T., Sevanto, S., Wong, S. C., McDowell, N. G., and Farquhar, G. D.: Unsaturation of vapour pressure inside leaves of two conifer species, Sci. Rep., 8, 7667–7673, 2018. 

Ciais, Ph., Reichstein, M., Viovy, N., Granier, A., Ogee, J., Allard, V., Aubinet, M., Buchmann, N., Bernhofer, Chr., Carrara, A., Chevallier, F., De Noblet, N., Friend, A. D., Friedlingstein, P., Grünwald, T., Heinesch, B., Keronen, P., Knohl, A., Krinner, G., and Loustau, D.: Europe-wide reduction in primary productivity caused by the heat and drought in 2003, Nature, 437, 529–533, https://doi.org/10.1038/nature03972, 2005. 

Cleverly, J., Boulain, N., Villalobos-Vega, R., Grant, N., Faux, R., Wood, C., Cook, P. G., Yu, Q., Leigh, A., and Eamus, D.: Dynamics of component carbon fluxes in a semi-arid Acacia woodland, central Australia, J. Geophys. Res.-Biogeo., 118, 1168–1185, 2013. 

Coumou, D. and Robinson, A.: Historic and future increase in the global land area affected by monthly heat extremes, Environ. Res. Lett., 8, 034018, https://doi.org/10.1088/1748-9326/8/3/034018, 2013. 

Cowan, I. R. and Farquhar, G. D.: Stomatal function in relation to leaf metabolism and environment, in: Integration of Activity in the Higher Plant, edited by: Jennings, D. H., Cambridge University Press, Cambridge, 471–505, 1977. 

Curtis, E. M., Gollan, J., Murray, B. R., and Leigh, A.: Native microhabitats better predict tolerance to warming than latitudinal macro-climatic variables in arid-zone plants, J. Biogeogr., 43, 1156–1165, 2016. 

De Kauwe, M. G., Medlyn, B. E., Zaehle, S., Walker, A. P., Dietze, M. C., Hickler, T., Jain, A. K., Luo, Y. , Parton, W. J., Prentice, I. C., Smith, B., Thornton, P. E., Wang, S., Wang, Y. , Wårlind, D., Weng, E., Crous, K. Y., Ellsworth, D. S., Hanson, P. J., Seok Kim, H., Warren, J. M., Oren, R., and Norby, R. J. : Forest water use and water use efficiency at elevated CO2: A model-data intercomparison at two contrasting temperate forest FACE sites, Glob. Change Biol., 19, 1759–1779, 2013. 

De Kauwe, M. G., Medlyn, B. E., Knauer, J., and Williams, C. A.: Ideas and perspectives: how coupled is the vegetation to the boundary layer?, Biogeosciences, 14, 4435–4453, https://doi.org/10.5194/bg-14-4435-2017, 2017. 

De Kauwe, M. G.: mdekauwe/heat_extremes_decoupling: accepted (Version v1.0), Zenodo, https://doi.org/10.5281/zenodo.2563373, 2019. 

Dekker, S. C., Bouten, W., and Schaap, M. G.: Analysing forest transpiration model errors with artificial neural networks, J. Hydrol., 246, 197–208, 2001. 

Dekker, S. C., Groenendijk, M., Booth, B. B. B., Huntingford, C., and Cox, P. M.: Spatial and temporal variations in plant water-use efficiency inferred from tree-ring, eddy covariance and atmospheric observations, Earth Syst. Dynam., 7, 525–533, https://doi.org/10.5194/esd-7-525-2016, 2016. 

Dirmeyer, P. A., Gao, X., Zhao, M., Guo, Z. H., Oki, T., and Hanasaki, N.: GSWP-2-multimodel analysis and implications for our perception of the land surface, B. Am. Meteorol. Soc., 87, 1381–1397, 2006. 

Donat, M. G., Pitman, A. J., and Seneviratne, S. I.: Regional warming of hot extremes accelerated by surface energy fluxes, Geophys. Res. Lett., 44, 7011–7019, 2017. 

Drake, J. E., Tjoelker, M. G., Aspinwall, M. J., Reich, P. B., Barton, C. V., Medlyn, B. E., and Duursma, R. A.: Does physiological acclimation to climate warming stabilize the ratio of canopy respiration to photosynthesis?, New Phytol., 211, 850–863, 2016. 

Drake, J. E., Vårhammar, A., Kumarathunge, D., Medlyn, B. E., Pfautsch, S., Reich, P. B., Tissue, D. T., Ghannoum, O., and Tjoelker, M. G.: A common thermal niche among geographically diverse populations of the widely distributed tree species Eucalyptus tereticornis: No evidence for adaptation to climate-of-origin, Glob. Change Biol., 23, 5069–5082, 2017. 

Drake, J. E., Tjoelker, M. G., Vårhammar, A., Medlyn, B. E., Reich, P. B., Leigh, A., Pfautsch, S., Blackman, C. J., López, R., Aspinwall, M. J., Crous, K. Y., Duursma, R. A., Kumarathunge D., De Kauwe, M. G., Jiang, M., Nicotra, A. B., Tissue, D. T., Choat, B., Atkin, O. K., and Barton, C. V. M.: Trees tolerate an extreme heatwave via sustained transpirational cooling and increased leaf thermal tolerance, Glob. Change Biol., 24, 2390–2402, 2018. 

Duursma, R. A., Barton, C. V. M., Lin, Y.-S., Medlyn, B. E., Eamus, D., Tissue, D. T., Ellsworth, D. S., and McMurtrie, R. E.: The peaked response of transpiration rate to vapour pressure deficit in field conditions can be explained by the temperature optimum of photosynthesis, Agr. Forest Meteorol., 189–190, 2–10, 2014. 

Eder, F., Schmidt, M., Damian, T., Träumner, K., and Mauder, M.: Mesoscale Eddies Affect Near-Surface Turbulent Exchange: Evidence from Lidar and Tower Measurements, J. Appl. Meteor. Climatol., 54, 189–206, 2015. 

Fischer, E., Seneviratne, S., Lüthi, D., and Schär, C.: Contribution of land-atmosphere coupling to recent European summer heat waves, Geophys. Res. Lett., 34, L06707, https://doi.org/10.1029/2006GL029068, 2007. 

Flach, M., Sippel, S., Gans, F., Bastos, A., Brenning, A., Reichstein, M., and Mahecha, M. D.: Contrasting biosphere responses to hydrometeorological extremes: revisiting the 2010 western Russian heatwave, Biogeosciences, 15, 6067–6085, https://doi.org/10.5194/bg-15-6067-2018, 2018. 

Foken, T.: The energy balance closure problem: an overview, Ecol. Appl., 18, 1351–1367, 2008. 

Griebel, A., Bennett, L. T., Metzen, D., Cleverly, J., Burba, G., and Arndt, S. K.: Effects of inhomogeneities within the flux footprint on the interpretation of seasonal, annual, and interannual ecosystem carbon exchange, Agr. Forest Meteorol., 221, 50–60, 2016. 

Groenendijk, M., Dolman, A. J., Ammann, C., Arneth, A., Cescatti, A., Dragoni, D., Gash, J. H. C., Gianelle, D., Gioli, B., Kiely, G., Knohl, A., Law, B. E., Lund, M., Marcolla, B., Van Der Molen, M. K., Montagnani, L., Moors, E., Richardson, A. D., Roupsard, O., Verbeeck, H., and Wohlfahrt, G.: Seasonal varia- tion of photosynthetic model parameters and leaf area index from global Fluxnet eddy covariance data, J. Geophys. Res., 116, 1–18, https://doi.org/10.1029/2011JG001742, 2011. 

Hendricks-Franssen, H. J., Stöckli, R., Lehner, I., Rotenberg, E., Seneviratne, S. I.: Energy balance closure of eddy-covariance data: a multisite analysis for European FLUXNET stations, Agr. Forest Meteorol., 150, 1553–1567, 2010. 

Isaac, P., Cleverly, J., McHugh, I., van Gorsel, E., Ewenz, C., and Beringer, J.: OzFlux data: network integration from collection to curation, Biogeosciences, 14, 2903–2928, https://doi.org/10.5194/bg-14-2903-2017, 2017. 

Jarvis, P. and McNaughton, K.: Stomatal control of transpiration: Scaling up from leaf to region, JAdv. Ecol. Res., 15, 1–49, 1986. 

Kala, J., De Kauwe, M. G., Pitman, A. J., Medlyn, B. E., Wang, Y.-P., Lorenz, R., and Perkins-Kirkpatrick, S. E.: Impact of the representation of stomatal conductance on model projections of heatwave intensity, Sci. Rep., 6, 1–7, 2016. 

Katul, G. G., Palmroth, S., and Oren, R.: Leaf stomatal responses to vapour pressure deficit under current and CO2-enriched atmosphere explained by the economics of gas exchange, Plant Cell Environ., 32, 968–979, 2009. 

Keenan T. F., Hollinger, D. Y., Bohrer, G., Dragoni, D., Munger, J. W., Schmid, H. P., Richardson, A. D., Keeling, B. C. D., Bacastow, R. B., Bainbridge, A. E., Ekdahl, C. A., Guenther, P. R., and Waterman, L. E. E. S.: Increase in forest water-use efficiency as atmospheric carbon dioxide concentrations rise, Nature, 499, 324–327, 2013. 

Klein Tank, A. M. G., Zwiers, F. W., and Zhang, X.: Guidelines on Analysis of extremes in a changing climate in support of informed decisions for adaptation, Geneva, Switzerland, 2009. 

Kowalski, A. S.: The boundary condition for vertical velocity and its interdependence with surface gas exchange, Atmos. Chem. Phys., 17, 8177–8187, https://doi.org/10.5194/acp-17-8177-2017, 2017. 

Knauer, J., Zaehle, S., Medlyn, B. E., Reichstein, M., Williams, C. A., Migliavacca, M., De Kauwe, M. G., Werner, C., Keitel, C., Kolari, P., Limousin, J.-M., and Linderson, M.-L.: Towards physiologically meaningful water-use efficiency estimates from eddy covariance data, Glob. Change Biol., 24, 694–710, 2017. 

Kumarathunge, D. P., Medlyn, B. E., Drake, J. E., Tjoelker, M. G., Aspinwall, M. J., Battaglia, M., Cano, F. J., Carter, K. R., Cavaleri, M. A., Cernusak, L. A., Chambers, J. Q., Crous, K. Y., De Kauwe, M. G., Dillaway, D. N., Dreyer, E., Ellsworth, D. S., Ghannoum, O., Han, Q., Hikosaka, K. Jensen, A. M., Kelly, J. W. G., Kruger, E. L., Mercado, L. M., Onoda, Y., Reich, P. B., Rogers, A., Slot, M., Smith, N. G., Tarvainen, L., Tissue, D. T., Togashi, H. F., Tribuzy, E. S., Uddling, J., Vårhammar, A., Wallin, G., Warren, J. M., and Way, D. A.: Acclimation and adaptation components of the temperature dependence of plant photosynthesis at the global scale, New Phytol., https://doi.org/10.1111/nph.15668, online first, 2019. 

Law, B. E., Falge, E., Gu, L., Baldocchi, D. D., Bakwin, P., Berbigier, P., Davis, K., Dolman, A. J., Falk, M., Fuentes, J. D., Goldstein, A., Granier, A., Grelle, A., Hollinger, D., Janssens, I. A., Jarvis, P., Jensen, N. O., Katul, G., Mahli, Y., Matteucci, G., Meyers, T., Monson, R., Munger, W., and Oechel, W.: Environmental controls over carbon dioxide and water vapor exchange of terrestrial vegetation, Agr. For. Meteorol., 113, 97–120, 2002. 

Lin, Y.-S., Medlyn, B. E., and Ellsworth, D. S.: Temperature responses of leaf net photosynthesis: The role of component processes, Tree Physiol., 32, 219–231, 2012. 

Lloyd, J.: Modeling stomatal responses to environment in Macadamia integrifolia, Aust. J. Plant Physiol., 18, 649–660, 1991. 

Lorenz, R., Jaeger, E. B., and Seneviratne, S. I.: Persistence of heat waves and its link to soil moisture memory, Geophys. Res. Lett., 37, L09703, https://doi.org/10.1029/2010GL042764, 2010. 

Macfarlane, C.: Great Western Woodlands OzFlux: Australian and New Zealand Flux Research and Monitoring, hdl: 102.100.100/14226, 2013. 

McHugh, I. D., Beringer, J., Cunningham, S. C., Baker, P. J., Cavagnaro, T. R., Mac Nally, R., and Thompson, R. M.: Interactions between nocturnal turbulent flux, storage and advection at an “ideal” eucalypt woodland site, Biogeosciences, 14, 3027–3050, https://doi.org/10.5194/bg-14-3027-2017, 2017. 

Medlyn, B. E., Duursma, R. A., Eamus, D., Ellsworth, D. S., Prentice, I. C., Barton, C. V., Crous, K. Y., De Angelis, P., Freeman, M., and Wingate, L.: Reconciling the optimal and empirical approaches to modelling stomatal conductance, Glob. Change Biol., 17, 2134–2144, 2011. 

Medlyn, B. E., De Kauwe, M. G., Lin, Y., Knauer, J., Duursma, R. A., Williams, C. A., Arneth, A., Clement, R., Isaac, P., Limousin, J., Linderson, M., Meir, P., Martin-StPaul, N., and Wingate, L.: How do leaf and ecosystem measures of water-use efficiency compare?, New Phytol., 216, 758–770, 2017. 

Merbold, L., Ardö, J., Arneth, A., Scholes, R. J., Nouvellon, Y., de Grandcourt, A., Archibald, S., Bonnefond, J. M., Boulain, N., Brueggemann, N., Bruemmer, C., Cappelaere, B., Ceschia, E., El-Khidir, H. A. M., El-Tahir, B. A., Falk, U., Lloyd, J., Kergoat, L., Le Dantec, V., Mougin, E., Muchinda, M., Mukelabai, M. M., Ramier, D., Roupsard, O., Timouk, F., Veenendaal, E. M., and Kutsch, W. L.: Precipitation as driver of carbon fluxes in 11 African ecosystems, Biogeosciences, 6, 1027–1041, https://doi.org/10.5194/bg-6-1027-2009, 2009. 

Meyer, W., Kondrlovà, E., and Koerber, G.: Evaporation of perennial semi-arid woodland in southeastern Australia is adapted for irregular but common dry periods, Hydrol. Proc., 29, 3714–3726, 2015. 

Miralles, D., van den Berg, M., Teuling, A., and de Jeu, R.: Soil moisture-temperature coupling: A multiscale observational analysis, Geophys. Res. Lett., 39, L21707, https://doi.org/10.1029/2012GL053703, 2012. 

Miralles, D. G., Teuling, A. J., Van Heerwaarden, C. C., and de Arellano, J. V.-G.: Mega-heatwave temperatures due to combined soil desiccation and atmospheric heat accumulation, Nat. Geosci., 7, 345–349, 2014. 

Oren, R., Sperry, J. S., Katul, G. G., Pataki, D. E., Ewers, B. E., Phillips, N., and Schäfer, K. V.: Survey and synthesis of intra- and interspecific variation in stomatal sensitivity to vapour pressure deficit, Plant Cell Environ., 22, 1515–1526, https://doi.org/10.1046/j.1365-3040.1999.00513.x, 1999. 

O'Sullivan, O. S., Heskel, M. A., Reich, P. B., Tjoelker, M. G., Weerasinghe, L. K., Penillard, A., Zhu, L., Egerton, J. J., Bloomfield, K. J., Creek, D., Bahar, N. H., Griffin, K. L., Hurry, V., Meir, P., Turnbull, M. H., and Atkin, O. K.: Thermal limits of leaf metabolism across biomes, Glob. Change Biol., 23, 209–223, 2017. 

Perkins, S., Alexander, L., and Nairn, J.: Increasing frequency, intensity and duration of observed global heatwaves and warm spells, Geophys. Res. Lett., 39, L20714, https://doi.org/10.1029/2012GL053361, 2012. 

Perkins, S. E.: A review on the scientific understanding of heatwaves—their measurement, driving mechanisms, and changes at the global scale, Atmos. Res., 164, 242–267, 2015. 

Rambal, S., Joffre, R., Ourcival, J., Cavender-Bares, J., and Rocheteau, A.: The growth respiration component in eddy CO2 flux from a quercus ilex mediterranean forest, Glob. Change Biol., 10, 1460–1469, 2004. 

Rey, A., Pegoraro, E., Tedeschi, V., De Parri, I., Jarvis, P. G., and Valentini, R.: Annual variation in soil respiration and its components in a coppice oak forest in central Italy, Glob. Change Biol., 8, 851–866, 2002. 

Sabbatini, S., Arriga, N., Bertolini, T., Castaldi, S., Chiti, T., Consalvo, C., Njakou Djomo, S., Gioli, B., Matteucci, G., and Papale, D.: Greenhouse gas balance of cropland conversion to bioenergy poplar short-rotation coppice, Biogeosciences, 13, 95–113, https://doi.org/10.5194/bg-13-95-2016, 2016. 

Schmid, H. P., Grimmond, C. S. B., Cropley, F., Offerle, B., and Su, H.-B.: Measurements of CO2 and energy fluxes over a mixed hardwood forest in the mid-western United States, Agr. Forest Meteorol., 103, 357–374, 2000. 

Seneviratne, S. I., Luethi, D., Litschi, M., and Schaer, C.: Land-atmosphere coupling and climate change in Europe, Nature, 443, 205–209, 2006. 

Silberstein, R.: Gingin OzFlux: Australian and New Zealand Flux Research and Monitoring, hdl: 102.100.100/22677, 2015. 

Sillmann, J., Kharin, V. V., Zwiers, F. W., Zhang, X., and Bronaugh, D.: Climate extremes indices in the CMIP5 multimodel ensemble: Part 2. Future climate projections, J. Geophys. Res.-Atmos., 118, 2473–2493, 2013. 

Sperry, J. S., Venturas, M. D., Anderegg, W. R., Mencuccini, M., Mackay, D. S., Wang, Y., and Love, D. M.: Predicting stomatal responses to the environment from the optimization of photosynthetic gain and hydraulic cost, Plant Cell Environ., 40, 816–830, 2017. 

Tatarinov, F., Rotenberg, E., Maseyk, K., Ogée, J., Klein, T., and Yakir, D.: Resilience to seasonal heat wave episodes in a Mediterranean pine forest, New Phytol., 210, 485–496, 2016. 

Teuling, A. J., Seneviratne, S. I., Stöckli, R., Reichstein, M., Moors, E., Ciais, P., Luyssaert, S., van den Hurk, B., Ammann, C., Bern- hofer, C., Dellwik, E., Gianelle, D., Gielen, B., Grünwald, T., Klumpp, K., Montagnani, L., Moureaux, C., Sottocornola, M., and Wohlfahrt, G.: Contrasting response of European forest and grassland energy exchange to heatwaves, Nat. Geosci., 3, 722–727, 2010. 

Trewavas, A.: What is plant behaviour? Plant Cell Environ., 32, 606–616, 2009. 

Ukkola, A. M., Haughton, N., De Kauwe, M. G., Abramowitz, G., and Pitman, A. J.: FluxnetLSM R package (v1.0): a community tool for processing FLUXNET data for use in land surface modelling, Geosci. Model Dev., 10, 3379–3390, https://doi.org/10.5194/gmd-10-3379-2017, 2017. 

Urban, J., Ingwers, M. W., McGuire, M. A., and Teskey, R. O.: Increase in leaf temperature opens stomata and decouples net photosynthesis from stomatal conductance in pinus taeda and populus deltoides × nigra, J. Exp. Bot., 68, 1757–1767, 2017. 

van Heerwaarden, C. C. and Teuling, A. J.: Disentangling the response of forest and grassland energy exchange to heatwaves under idealized land–atmosphere coupling, Biogeosciences, 11, 6159–6171, https://doi.org/10.5194/bg-11-6159-2014, 2014. 

von Buttlar, J., Zscheischler, J., Rammig, A., Sippel, S., Reichstein, M., Knohl, A., Jung, M., Menzer, O., Arain, M. A., Buchmann, N., Cescatti, A., Gianelle, D., Kiely, G., Law, B. E., Magliulo, V., Margolis, H., McCaughey, H., Merbold, L., Migliavacca, M., Montagnani, L., Oechel, W., Pavelka, M., Peichl, M., Rambal, S., Raschi, A., Scott, R. L., Vaccari, F. P., van Gorsel, E., Varlagin, A., Wohlfahrt, G., and Mahecha, M. D.: Impacts of droughts and extreme-temperature events on gross primary production and ecosystem respiration: a systematic assessment across ecosystems and climate zones, Biogeosciences, 15, 1293–1318, https://doi.org/10.5194/bg-15-1293-2018, 2018. 

von Caemmerer, S. and Evans, J. R.: Temperature responses of mesophyll conductance differ greatly between species, Plant Cell Environ., 38, 629–637, 2015. 

Weston, D. J. and Bauerle, W. L.: Inhibition and acclimation of C3 photosynthesis to moderate heat: a perspective from thermally contrasting genotypes of Acer rubrum L. (red maple), Tree Physiol., 27, 1083–1092, 2007. 

Williams, M., Bond, B., and Ryan, M.: Evaluating different soil and plant hydraulic constraints on tree function using a model and sap flow data from ponderosa pine, Plant Cell Amp. Environ., 24, 679–690, 2001. 

Wilson, K., Goldstein, A., Falge, E., Aubinet, M., Baldocchi, D., Berbigier, P., Bernhofer, C., Ceulemans, R., Dolman, H., Field, C., Grelle, A., Ibrom, A., Law, B. E., Kowalski, A., Meyers, T., Moncrieff, J., Monson, R., Oechel, W., Tenhunen, J., Valentini, R., and Verma, S.: Energy balance closure at FLUXNET sites, Agr. Forest Meteorol., 113, 223–243, 2002.  

Wohlfahrt, G., Haslwanter, A., Hörtnagl, L., Jasoni, R. L., Fenster- maker, L. F., Arnone, J. A., and Hammerle, A.: On the consequences of the energy imbalance for calculating surface conductance to water vapour, Agr. Forest Meteorol., 149, 1556–1559, 2009. 

Wolf, S., Eugster, W., Ammann, C., Häni, M., Zielis, S., Hiller, R., Stieger, J., Imer, D., Merbold, L., and Buchmann, N.: Contrasting response of grassland versus forest carbon and water fluxes to spring drought in Switzerland, Environ. Res. Lett., 8, 035007, https://doi.org/10.1088/1748-9326/8/3/035007, 2013. 

Wolf, A., Anderegg, W. R. L., and Pacala, S. W.: Optimal stomatal behavior with competition for water and risk of hydraulic impairment, P. Natl. Acad. Sci. USA, 113, E7222–E7230, https://doi.org/10.1073/pnas.1615144113, 2016 

Yu, G.-R., Wen, X.-F., Sun, X.-M., Tanner, B. D., Lee, X., and Chen, J.-Y.: Overview of Chinaflux and evaluation of its eddy covariance measurement, Agr. Forest Meteorol., 137, 125–137, 2006. 

Zhou, S., Yu, B., Huang, Y., and Wang, G.: The effect of vapor pressure deficit on water use efficiency at the subdaily time scale, Geophys. Res. Lett., 41, 5005–5013, https://doi.org/10.1002/2014GL060741, 2014. 

Zhou, S., Yu, B., Huang, Y., and Wang, G.: Daily underlying water use efficiency for AmeriFlux sites, J. Geophys. Res.-Biogeosci., 120, 887–902, https://doi.org/10.1002/2015JG002947, 2015. 

Zhu, L., Bloomfield, K. J., Hocart, C. H., Egerton, J. J. G., O'Sullivan, O. S., Penillard, A., Weerasinghe, L. K., and Atkin, O. K.: Plasticity of photosynthetic heat tolerance in plants adapted to thermally contrasting biomes, Plant Cell Environ., 18, 1251–1262, 2018. 

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Short summary
Recent experimental evidence suggests that during heat extremes, trees may reduce photosynthesis to near zero but increase transpiration. Using eddy covariance data and examining the 3 days leading up to a temperature extreme, we found evidence of reduced photosynthesis and sustained or increased latent heat fluxes at Australian wooded flux sites. However, when focusing on heatwaves, we were unable to disentangle photosynthetic decoupling from the effect of increasing vapour pressure deficit.
Recent experimental evidence suggests that during heat extremes, trees may reduce photosynthesis...
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