Articles | Volume 15, issue 5
https://doi.org/10.5194/bg-15-1415-2018
https://doi.org/10.5194/bg-15-1415-2018
Research article
 | 
08 Mar 2018
Research article |  | 08 Mar 2018

Revisiting chlorophyll extraction methods in biological soil crusts – methodology for determination of chlorophyll a and chlorophyll a + b as compared to previous methods

Jennifer Caesar, Alexandra Tamm, Nina Ruckteschler, Anna Lena Leifke, and Bettina Weber

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Latest update: 28 Mar 2024
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Short summary
In our study we analyzed the efficiency of different chlorophyll extraction solvents and investigated the effect of different preparatory steps to determine the optimal extraction method for biological soil crusts. Based on our results we confirm a DMSO-based chlorophyll extraction method without grinding pretreatment and suggest to insert an intermediate shaking step for complete chlorophyll extraction.
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