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Biogeosciences An interactive open-access journal of the European Geosciences Union
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Volume 14, issue 14
Biogeosciences, 14, 3431–3444, 2017
https://doi.org/10.5194/bg-14-3431-2017
© Author(s) 2017. This work is distributed under
the Creative Commons Attribution 3.0 License.

Special issue: Ecosystem processes and functioning across current and future...

Biogeosciences, 14, 3431–3444, 2017
https://doi.org/10.5194/bg-14-3431-2017
© Author(s) 2017. This work is distributed under
the Creative Commons Attribution 3.0 License.

Research article 20 Jul 2017

Research article | 20 Jul 2017

Interaction of CO2 concentrations and water stress in semiarid plants causes diverging response in instantaneous water use efficiency and carbon isotope composition

Na Zhao1,3, Ping Meng2, Yabing He1, and Xinxiao Yu1,3 Na Zhao et al.
  • 1College of soil and water conservation, Beijing Forestry University, Beijing 100083, People's Republic of China
  • 2Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, People's Republic of China
  • 3Beijing collaborative innovation center for eco-environmental improvement with forestry and fruit trees, Beijing, People's Republic of China

Abstract. In the context of global warming attributable to the increasing levels of CO2, severe drought may be more frequent in areas that already experience chronic water shortages (semiarid areas). This necessitates research on the interactions between increased levels of CO2 and drought and their effect on plant photosynthesis. It is commonly reported that 13C fractionation occurs as CO2 gas diffuses from the atmosphere to the substomatal cavity. Few researchers have investigated 13C fractionation at the site of carboxylation to cytoplasm before sugars are exported outward from the leaf. This process typically progresses in response to variations in environmental conditions (i.e., CO2 concentrations and water stress), including in their interaction. Therefore, saplings of two typical plant species (Platycladus orientalis and Quercus variabilis) from semiarid areas of northern China were selected and cultivated in growth chambers with orthogonal treatments (four CO2 concentration ([CO2])  ×  five soil volumetric water content (SWC)). The δ13C of water-soluble compounds extracted from leaves of saplings was determined for an assessment of instantaneous water use efficiency (WUEcp) after cultivation. Instantaneous water use efficiency derived from gas-exchange measurements (WUEge) was integrated to estimate differences in δ13C signal variation before leaf-level translocation of primary assimilates. The WUEge values in P. orientalis and Q.  variabilis both decreased with increased soil moisture at 35–80 % of field capacity (FC) and increased with elevated [CO2] by increasing photosynthetic capacity and reducing transpiration. Instantaneous water use efficiency (iWUE) according to environmental changes differed between the two species. The WUEge in P. orientalis was significantly greater than that in Q. variabilis, while an opposite tendency was observed when comparing WUEcp between the two species. Total 13C fractionation at the site of carboxylation to cytoplasm before sugar export (total 13C fractionation) was species-specific, as demonstrated in the interaction of [CO2] and SWC. Rising [CO2] coupled with moistened soil generated increasing disparities in δ13C between water-soluble compounds (δ13CWSC) and estimates based on gas-exchange observations (δ13Cobs) in P. orientalis, ranging between 0.0328 and 0.0472 ‰. Differences between δ13CWSC and δ13Cobs in Q. variabilis increased as [CO2] and SWC increased (0.0384–0.0466 ‰). The 13C fractionation from mesophyll conductance (gm) and post-carboxylation both contributed to the total 13C fractionation that was determined by δ13C of water-soluble compounds and gas-exchange measurements. Total 13C fractionation was linearly dependent on stomatal conductance, indicating that post-carboxylation fractionation could be attributed to environmental variation. The magnitude and environmental dependence of apparent post-carboxylation fractionation is worth our attention when addressing photosynthetic fractionation.

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Few researchers have investigated post-photosynthetic 13C fractionation happening in leaves. Potted saplings were cultivated under orthogonal treatments of CO2 concentration and soil water content. The 13C fractionation from mesophyll conductance and post-carboxylation both contributed to the total 13C fractionation that was determined by δ13C of water-soluble compounds and gas-exchange measurements.
Few researchers have investigated post-photosynthetic 13C fractionation happening in leaves....
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