Journal cover Journal topic
Biogeosciences An interactive open-access journal of the European Geosciences Union
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Volume 13, issue 19
Biogeosciences, 13, 5527–5539, 2016
https://doi.org/10.5194/bg-13-5527-2016
© Author(s) 2016. This work is distributed under
the Creative Commons Attribution 3.0 License.
Biogeosciences, 13, 5527–5539, 2016
https://doi.org/10.5194/bg-13-5527-2016
© Author(s) 2016. This work is distributed under
the Creative Commons Attribution 3.0 License.

Research article 06 Oct 2016

Research article | 06 Oct 2016

Seasonal changes in the D  /  H ratio of fatty acids of pelagic microorganisms in the coastal North Sea

Sandra Mariam Heinzelmann1, Nicole Jane Bale1, Laura Villanueva1, Danielle Sinke-Schoen1, Catharina Johanna Maria Philippart2,3, Jaap Smede Sinninghe Damsté1,4, Stefan Schouten1,4, and Marcel Teunis Jan van der Meer1 Sandra Mariam Heinzelmann et al.
  • 1Department of Marine Microbiology and Biogeochemistry, NIOZ Royal Netherlands Institute for Sea Research and Utrecht University, P.O. Box 59, 1790 AB Den Burg, the Netherlands
  • 2Department of Coastal Systems Sciences, NIOZ Royal Netherlands Institute for Sea Research and Utrecht University, P.O. Box 59, 1790 AB Den Burg, the Netherlands
  • 3Utrecht University, Faculty of Geosciences, Department of Physical Geography, Coastal Processes, P.O. Box 80.115, 3508 TC Utrecht, the Netherlands
  • 4Utrecht University, Faculty of Geosciences, Department of Earth Sciences, Geochemistry, P.O. Box 80.021, 3508 TA Utrecht, the Netherlands

Abstract. Culture studies of microorganisms have shown that the hydrogen isotopic composition of fatty acids depends on their metabolism, but there are only few environmental studies available to confirm this observation. Here we studied the seasonal variability of the deuterium-to-hydrogen (D / H) ratio of fatty acids in the coastal Dutch North Sea and compared this with the diversity of the phyto- and bacterioplankton. Over the year, the stable hydrogen isotopic fractionation factor ε between fatty acids and water (εlipid/water) ranged between −172 and −237 ‰, the algal-derived polyunsaturated fatty acid nC20:5 generally being the most D-depleted (−177 to −235 ‰) and nC18:0 the least D-depleted fatty acid (−172 to −210 ‰). The in general highly D-depleted nC20:5 is in agreement with culture studies, which indicates that photoautotrophic microorganisms produce fatty acids which are significantly depleted in D relative to water. The εlipid/water of all fatty acids showed a transient shift towards increased fractionation during the spring phytoplankton bloom, indicated by increasing chlorophyll a concentrations and relative abundance of the nC20:5 polyunsaturated fatty acids, suggesting increased contributions of photoautotrophy. Time periods with decreased fractionation (less negative εlipid/water values) can potentially be explained by an increased contribution of heterotrophy to the fatty acid pool. Our results show that the hydrogen isotopic composition of fatty acids is a promising tool to assess the community metabolism of coastal plankton potentially in combination with the isotopic analysis of more specific biomarker lipids.<

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In order to understand microbial communities in the environment it is necessary to assess their metabolic potential. The hydrogen isotopic composition of fatty acids has been shown to be promising tool to study the general metabolism of microorganisms in pure culture. Here we showed that it is possible to study seasonal changes in the general metabolism of the whole community by studying the hydrogen isotopic composition of fatty acids.
In order to understand microbial communities in the environment it is necessary to assess their...
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