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Biogeosciences An interactive open-access journal of the European Geosciences Union
Biogeosciences, 13, 3187-3202, 2016
https://doi.org/10.5194/bg-13-3187-2016
© Author(s) 2016. This work is distributed under
the Creative Commons Attribution 3.0 License.
Research article
02 Jun 2016
Heterotrophic bacterial production and metabolic balance during the VAHINE mesocosm experiment in the New Caledonia lagoon
France Van Wambeke1, Ulrike Pfreundt2, Aude Barani1, Hugo Berthelot1, Thierry Moutin1, Martine Rodier3,a, Wolfgang R. Hess2, and Sophie Bonnet1,3 1Aix Marseille Université, CNRS/INSU, Université de Toulon, IRD, Mediterranean Institute of Oceanography (MIO) UM110, 13288, Marseille, France
2University of Freiburg, Faculty of Biology, Schaenzlestr. 1, 79104 Freiburg, Germany
3Mediterranean Institute of Oceanography (MIO) – IRD/CNRS/Aix-Marseille University IRD Nouméa, 101 Promenade R. Laroque, BPA5, 98848, Nouméa CEDEX, New Caledonia, France
anow at: IRD, Université de la Polynésie française – Institut Malardé – Ifremer, UMR 241 Ecosystèmes Insulaires Océaniens (EIO), IRD Tahiti, PB 529, 98713 Papeete, Tahiti, French Polynesia
Abstract. Studies investigating the fate of diazotrophs through the microbial food web are lacking, although N2 fixation can fuel up to 50 % of new production in some oligotrophic oceans. In particular, the role played by heterotrophic prokaryotes in this transfer is largely unknown. In the frame of the VAHINE (VAriability of vertical and tropHIc transfer of diazotroph derived N in the south wEst Pacific) experiment, three replicate large-volume (∼ 50 m3) mesocosms were deployed for 23 days in the new Caledonia lagoon and were intentionally fertilized on day 4 with dissolved inorganic phosphorus (DIP) to stimulate N2 fixation. We specifically examined relationships between heterotrophic bacterial production (BP) and N2 fixation or primary production, determined bacterial growth efficiency and established carbon budgets. BP was statistically higher during the second phase of the experiment (P2: days 15–23), when chlorophyll biomass started to increase compared to the first phase (P1: days 5–14). Phosphatase alkaline activity increased drastically during the second phase of the experiment, showing adaptations of microbial populations after utilization of the added DIP. Notably, among autotrophs, Synechococcus abundances increased during P2, possibly related to its capacity to assimilate leucine and to produce alkaline phosphatase. Bacterial growth efficiency based on the carbon budget (27–43 %), was notably higher than generally cited for oligotrophic environments and discussed in links with the presence of abundant species of bacteria expressing proteorhodopsin. The main fates of gross primary production (particulate + dissolved) were respiration (67 %) and export through sedimentation (17 %). BP was highly correlated with particulate primary production and chlorophyll biomass during both phases of the experiment but was slightly correlated, and only during P2 phase, with N2 fixation rates. Heterotrophic bacterial production was strongly stimulated after mineral N enrichment experiments, suggesting N-limitation of heterotrophic bacteria across the experiment. N2 fixation rates corresponded to 17–37 % of the nitrogen demand of heterotrophic bacteria. Our results suggest that most of the diazotroph-derived nitrogen fuelled the heterotrophic bacterial community through indirect processes generating dissolved organic matter and detritus, like mortality, lysis and grazing of both diazotrophs and non-diazotrophs.

Citation: Van Wambeke, F., Pfreundt, U., Barani, A., Berthelot, H., Moutin, T., Rodier, M., Hess, W. R., and Bonnet, S.: Heterotrophic bacterial production and metabolic balance during the VAHINE mesocosm experiment in the New Caledonia lagoon, Biogeosciences, 13, 3187-3202, https://doi.org/10.5194/bg-13-3187-2016, 2016.
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Short summary
The phytoplankton is at the base of the plankton food web in large parts of oceanic "deserts" such as the South Pacific Ocean, where nitrogen sources limit activity. Mesocosms were fertilized with phosphorus to stimulate diazotrophy (atmospheric N2 fixation). Mostly diazotroph-derived nitrogen fuelled the heterotrophic bacterial community through indirect processes generating dissolved organic matter and detritus, such as mortality, lysis and grazing of both diazotrophs and non-diazotrophs.
The phytoplankton is at the base of the plankton food web in large parts of oceanic "deserts"...
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