Articles | Volume 12, issue 18
https://doi.org/10.5194/bg-12-5515-2015
https://doi.org/10.5194/bg-12-5515-2015
Technical note
 | 
28 Sep 2015
Technical note |  | 28 Sep 2015

Technical Note: Towards resolving in situ, centimeter-scale location and timing of biomineralization in calcareous meiobenthos – the calcein–osmotic pump method

J. M. Bernhard, W. G. Phalen, A. McIntyre-Wressnig, F. Mezzo, J. C. Wit, M. Jeglinski, and H. L. Filipsson

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Cited articles

Allen, K. A., and Hönisch, B.: The planktic foraminiferal B/Ca proxy for seawater carbonate chemistry: A critical evaluation, Earth Planet. Sci. Lett., 345, 203–211, 2012.
Apotheloz-Perret-Gentil, L., Holzmann, M., and Pawlowski, J.: Arnoldiellina fluorescens gen. et sp. nov. – A new green autofluorescent foraminifer from the Gulf of Eilat (Israel), Europ. J. Protistol., 49, 210–216, 2013.
Bernhard, J. M., Blanks, J. K., Hintz, C. J., and Chandler, G. T.: Use of the fluorescent calcite marker calcein to label foraminiferal tests, J. Foraminifer. Res., 34, 96–101, 2004.
Bernhard, J. M., Martin, J. B., and Rathburn, A. E.: Combined carbonate carbon isotopic and cellular ultrastructural studies of individual benthic foraminifera: 2. Toward an understanding of apparent disequilibrium in hydrocarbon seeps, Paleoceanography, 25, Pa4206, https://doi.org/10.1029/2010pa001930, 2010.
Bijma, J., Spero, H. J., and Lea, D. W.: Reassessing foraminiferal stable isotopes geochemistry: Impact of the ocean carbonate system (experimental results), in: Uses of proxies in paleoceanography: Examples from the South Atlantic, edited by: Fischer, G. and Wefer, G., Springer-Verlag, Berlin, 489–512, 1999.
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Short summary
We present an innovative method using osmotic pumps and the fluorescent marker calcein to help identify where and when calcareous bottom-dwelling organisms mineralize in sediments. These organisms, and their geochemical signatures in their carbonate, are the ocean’s storytellers helping us understand past marine conditions. For many species, the timing and location of their calcite growth is not known. Knowing this will enable us to reconstruct past marine environments with greater accuracy.
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