Technical Note: Towards resolving in situ, centimeter-scale location and timing of biomineralization in calcareous meiobenthos &ndash; the calcein–osmotic pump method

Bernhard, J. M.; Phalen, W. G.; McIntyre-Wressnig, A.; Mezzo, F.; Wit, J. C.; Jeglinski, M.; Filipsson, H. L.

doi:https://doi.org/10.5194/bg-12-5515-2015

Articles | Volume 12, issue 18

https://doi.org/10.5194/bg-12-5515-2015

© Author(s) 2015. This work is distributed under
the Creative Commons Attribution 3.0 License.

https://doi.org/10.5194/bg-12-5515-2015

© Author(s) 2015. This work is distributed under
the Creative Commons Attribution 3.0 License.

Articles | Volume 12, issue 18

Technical note

|

28 Sep 2015

Technical note |

| 28 Sep 2015

Technical Note: Towards resolving in situ, centimeter-scale location and timing of biomineralization in calcareous meiobenthos – the calcein–osmotic pump method

J. M. Bernhard, W. G. Phalen, A. McIntyre-Wressnig, F. Mezzo, J. C. Wit, M. Jeglinski, and H. L. Filipsson

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Final revised paper (published on 28 Sep 2015)
Preprint (discussion started on 25 Jun 2015)

Interactive discussion

Status: closed

AC: Author comment | RC: Referee comment | SC: Short comment | EC: Editor comment

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- Supplement

RC C3755: 'Referee comments on the manuscript bg-2015-213', Anonymous Referee #2, 22 Jul 2015
- AC C5088: 'Response to Reviewer #2', Joan Bernhard, 08 Sep 2015
RC C4675: 'Review Technical Note Bernhard et al.', Lennart de Nooijer, 25 Aug 2015
- AC C5085: 'Response to Review by L. de Nooijer', Joan Bernhard, 08 Sep 2015

Peer-review completion

AR: Author's response | RR: Referee report | ED: Editor decision

ED: Publish as is (09 Sep 2015) by Hiroshi Kitazato

AR by Joan Bernhard on behalf of the Authors (10 Sep 2015) Author's response

Short summary

We present an innovative method using osmotic pumps and the fluorescent marker calcein to help identify where and when calcareous bottom-dwelling organisms mineralize in sediments. These organisms, and their geochemical signatures in their carbonate, are the ocean’s storytellers helping us understand past marine conditions. For many species, the timing and location of their calcite growth is not known. Knowing this will enable us to reconstruct past marine environments with greater accuracy.