Articles | Volume 12, issue 6
https://doi.org/10.5194/bg-12-1865-2015
https://doi.org/10.5194/bg-12-1865-2015
Research article
 | 
20 Mar 2015
Research article |  | 20 Mar 2015

Multi-isotope labelling of organic matter by diffusion of 2H/18O-H2O vapour and 13C-CO2 into the leaves and its distribution within the plant

M. S. Studer, R. T. W. Siegwolf, M. Leuenberger, and S. Abiven

Related authors

Carbon transfer, partitioning and residence time in the plant-soil system: a comparison of two 13CO2 labelling techniques
M. S. Studer, R. T. W. Siegwolf, and S. Abiven
Biogeosciences, 11, 1637–1648, https://doi.org/10.5194/bg-11-1637-2014,https://doi.org/10.5194/bg-11-1637-2014, 2014

Related subject area

Biogeochemistry: Stable Isotopes & Other Tracers
Climatic controls on leaf wax hydrogen isotope ratios in terrestrial and marine sediments along a hyperarid-to-humid gradient
Nestor Gaviria-Lugo, Charlotte Läuchli, Hella Wittmann, Anne Bernhardt, Patrick Frings, Mahyar Mohtadi, Oliver Rach, and Dirk Sachse
Biogeosciences, 20, 4433–4453, https://doi.org/10.5194/bg-20-4433-2023,https://doi.org/10.5194/bg-20-4433-2023, 2023
Short summary
Fractionation of stable carbon isotopes during microbial propionate consumption in anoxic rice paddy soils
Ralf Conrad and Peter Claus
Biogeosciences, 20, 3625–3635, https://doi.org/10.5194/bg-20-3625-2023,https://doi.org/10.5194/bg-20-3625-2023, 2023
Short summary
Sources and sinks of carbonyl sulfide inferred from tower and mobile atmospheric observations in the Netherlands
Alessandro Zanchetta, Linda M. J. Kooijmans, Steven van Heuven, Andrea Scifo, Hubertus A. Scheeren, Ivan Mammarella, Ute Karstens, Jin Ma, Maarten Krol, and Huilin Chen
Biogeosciences, 20, 3539–3553, https://doi.org/10.5194/bg-20-3539-2023,https://doi.org/10.5194/bg-20-3539-2023, 2023
Short summary
Downpour dynamics: outsized impacts of storm events on unprocessed atmospheric nitrate export in an urban watershed
Joel T. Bostic, David M. Nelson, and Keith N. Eshleman
Biogeosciences, 20, 2485–2498, https://doi.org/10.5194/bg-20-2485-2023,https://doi.org/10.5194/bg-20-2485-2023, 2023
Short summary
The hidden role of dissolved organic carbon in the biogeochemical cycle of carbon in modern redox-stratified lakes
Robin Havas, Christophe Thomazo, Miguel Iniesto, Didier Jézéquel, David Moreira, Rosaluz Tavera, Jeanne Caumartin, Elodie Muller, Purificación López-García, and Karim Benzerara
Biogeosciences, 20, 2405–2424, https://doi.org/10.5194/bg-20-2405-2023,https://doi.org/10.5194/bg-20-2405-2023, 2023
Short summary

Cited articles

Augusti, A. and Schleucher, J.: The ins and outs of stable isotopes in plants, New Phytol., 174, 473–475, 2007.
Augusti, A., Betson, T. R., and Schleucher, J.: Hydrogen exchange during cellulose synthesis distinguishes climatic and biochemical isotope fractionations in tree rings, New Phytol., 172, 490–499, 2006.
Badeck, F.-W., Tcherkez, G., Nogués, S., Piel, C., and Ghashghaie, J.: Post-photosynthetic fractionation of stable carbon isotopes between plant organs – a widespread phenomenon, Rapid Commun. Mass Spectrom., 19, 1381–1391, 2005.
Barbour, M. M.: Stable oxygen isotope composition of plant tissue: a review, Funct. Plant Biol., 34, 83–94, 2007.
Bird, J. A. and Torn, M. S.: Fine roots vs. needles: a comparison of 13C and 15N dynamics in a ponderosa pine forest soil, Biogeochemistry, 79, 361–382, 2006.
Download
Short summary
We present a new technique to label organic matter (OM) at its place of formation by the application of 13C, 18O and 2H through the gaseous phase. The label diffused into leaves was incorporated into assimilates and was detected in plant tissues. This technique can be applied in soil sciences, e.g. to trace the decomposition pathways of soil OM inputs, or in plant physiology and palaeoclimatic reconstruction, e.g. to further investigate the origin of the 18O and 2H signal in tree ring cellulose.
Altmetrics
Final-revised paper
Preprint