Articles | Volume 12, issue 6
https://doi.org/10.5194/bg-12-1865-2015
https://doi.org/10.5194/bg-12-1865-2015
Research article
 | 
20 Mar 2015
Research article |  | 20 Mar 2015

Multi-isotope labelling of organic matter by diffusion of 2H/18O-H2O vapour and 13C-CO2 into the leaves and its distribution within the plant

M. S. Studer, R. T. W. Siegwolf, M. Leuenberger, and S. Abiven

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AR by Mirjam S. Studer on behalf of the Authors (20 Feb 2015)  Author's response    Manuscript
ED: Reconsider after major revisions (24 Feb 2015) by Xinming Wang
AR by Mirjam S. Studer on behalf of the Authors (26 Feb 2015)  Author's response    Manuscript
ED: Publish as is (02 Mar 2015) by Xinming Wang
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Short summary
We present a new technique to label organic matter (OM) at its place of formation by the application of 13C, 18O and 2H through the gaseous phase. The label diffused into leaves was incorporated into assimilates and was detected in plant tissues. This technique can be applied in soil sciences, e.g. to trace the decomposition pathways of soil OM inputs, or in plant physiology and palaeoclimatic reconstruction, e.g. to further investigate the origin of the 18O and 2H signal in tree ring cellulose.
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