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Biogeosciences An interactive open-access journal of the European Geosciences Union
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Volume 12, issue 4 | Copyright

Special issue: Geological and biological development of volcanic islands

Biogeosciences, 12, 1191-1203, 2015
© Author(s) 2015. This work is distributed under
the Creative Commons Attribution 3.0 License.

Research article 24 Feb 2015

Research article | 24 Feb 2015

Microbial colonization in diverse surface soil types in Surtsey and diversity analysis of its subsurface microbiota

V. Marteinsson1,3, A. Klonowski1, E. Reynisson1, P. Vannier1, B. D. Sigurdsson3, and M. Ólafsson2 V. Marteinsson et al.
  • 1Matís, Food safety, Environment & Genetics, Vínlandsleiđ 12, 113 Reykjavík, Iceland
  • 2ÍSOR, Gensásvegi 9, 108 Reykjavík, Iceland
  • 3Agricultural University of Iceland, Hvanneyri, 311 Borgarnes, Iceland

Abstract. Colonization of life on Surtsey has been observed systematically since the formation of the island 50 years ago. Although the first colonisers were prokaryotes, such as bacteria and blue–green algae, most studies have been focused on the settlement of plants and animals but less on microbial succession. To explore microbial colonization in diverse soils and the influence of associated vegetation and birds on numbers of environmental bacteria, we collected 45 samples from different soil types on the surface of the island. Total viable bacterial counts were performed with the plate count method at 22, 30 and 37 °C for all soil samples, and the amount of organic matter and nitrogen (N) was measured. Selected samples were also tested for coliforms, faecal coliforms and aerobic and anaerobic bacteria. The subsurface biosphere was investigated by collecting liquid subsurface samples from a 181 m borehole with a special sampler. Diversity analysis of uncultivated biota in samples was performed by 16S rRNA gene sequences analysis and cultivation. Correlation was observed between nutrient deficits and the number of microorganisms in surface soil samples. The lowest number of bacteria (1 × 104–1 × 105 cells g−1) was detected in almost pure pumice but the count was significantly higher (1 × 106–1 × 109 cells g−1) in vegetated soil or pumice with bird droppings. The number of faecal bacteria correlated also to the total number of bacteria and type of soil. Bacteria belonging to Enterobacteriaceae were only detected in vegetated samples and samples containing bird droppings. The human pathogens Salmonella, Campylobacter and Listeria were not in any sample. Both thermophilic bacteria and archaea 16S rDNA sequences were found in the subsurface samples collected at 145 and 172 m depth at 80 and 54 °C, respectively, but no growth was observed in enrichments. The microbiota sequences generally showed low affiliation to any known 16S rRNA gene sequences.

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Colonization of life on Surtsey has been observed systematically since the formation of the island. Microbial colonization and the influence of associate vegetation and birds on viable counts of environmental bacteria at the surface of the Surtsey was explored for the first time in diverse surface soils. Also, hot subsurface samples deep in the centre of this volcanic island were collected. Both uncultivated bacteria and archaea were found in the subsurface samples collected below 145 m.
Colonization of life on Surtsey has been observed systematically since the formation of the...