Journal cover Journal topic
Biogeosciences An interactive open-access journal of the European Geosciences Union
Biogeosciences, 11, 6277-6292, 2014
© Author(s) 2014. This work is distributed under
the Creative Commons Attribution 3.0 License.
Research article
21 Nov 2014
Retrieval of the photochemical reflectance index for assessing xanthophyll cycle activity: a comparison of near-surface optical sensors
A. Harris1, J. A. Gamon2, G. Z. Pastorello3, and C. Y. S. Wong2 1Geography, School of Environment, Education and Development, University of Manchester, Manchester, M13 9PL, UK
2Departments of Earth & Atmospheric Sciences and Biological Sciences, University of Alberta, Edmonton, AB T6G 2E3, Canada
3Computational Research Division, Lawrence Berkeley National Laboratory, CA 94720, USA
Abstract. Unattended optical sensors are increasingly being deployed on eddy covariance flux towers and are often used to complement existing vegetation and micrometeorological measurements to enable assessment of biophysical states and biogeochemical processes over a range of spatial scales. Of particular interest are sensors that can measure the photochemical reflectance index (PRI), which can provide information pertaining to leaf pigments and photosynthetic activity. This interest has facilitated the production of a new range of lower-cost multispectral sensors specifically designed to measure temporal changes in the PRI signal. However, little is known about the characteristics (spectral, radiometric and temporal) of many of these PRI sensors, making it difficult to compare data obtained from these sensors across time, geographical locations and instruments. Furthermore, direct testing of the capability of these sensors to actually detect the conversion of the xanthophyll cycle, which is the original biological basis of the PRI diurnal signal, is largely absent, often resulting in an unclear interpretation of the signal, particularly given the wide range of factors now known to influence PRI. Through a series of experiments, we assess the sensitivity of one of the leading brands of PRI sensor (Skye SKR 1800) to changes in vegetation photosynthetic activity in response to changing irradiance. We compare the results with those obtained using a more expensive industry-standard visible to near-infrared hyperspectral spectrometer (PP Systems UniSpec) and determine the radiometric compatibility of measurements made by the different instruments. Results suggest that the SKR 1800 instrument is able to track rapid (seconds to minutes) and more gradual diurnal changes in photosynthetic activity associated with xanthophyll cycle pigment conversion. Measurements obtained from both the high and lower cost instrument were significantly linearly correlated but were subject to a large systematic bias, illustrating that differences in instrument configuration (e.g. spectral response functions and band positions) can have a large impact on the PRI measurement values obtained. Despite differences in absolute PRI values, significant correlations were observed between the canopy PRI derived from both the SKR 1800 and the UniSpec instruments, and the epoxidation state of the xanthophyll cycle (r2 = 0.46 p < 0.05 and r2 = 0.76 p < 0.01, respectively). However, the dynamic range of the SKR 1800 PRI signal was often lower than more expensive instruments and thus the lower cost multispectral instrument may be less sensitive to pigment dynamics related to photosynthetic activity. Based on our findings, we make a series of recommendations for the effective use of such sensors under field conditions and advocate that sensors should be fully characterized prior to their field deployment.

Citation: Harris, A., Gamon, J. A., Pastorello, G. Z., and Wong, C. Y. S.: Retrieval of the photochemical reflectance index for assessing xanthophyll cycle activity: a comparison of near-surface optical sensors, Biogeosciences, 11, 6277-6292,, 2014.
Publications Copernicus
Short summary
Lower-cost optical sensors, which can be left outdoors, are often used to provide information regarding plant photosynthetic activity. A lower-cost sensor was compared with an expensive instrument to see if the data collected were comparable. Both instruments were able to track changes in photosynthetic activity, but the values recorded by each were different. This can cause difficulties when comparing data across space and time. We provide advice on how best to use these sensors in the field.
Lower-cost optical sensors, which can be left outdoors, are often used to provide information...