To evaluate the role of the razor clam <i>Ensis directus</i> in Dutch coastal waters, understanding its population dynamics is important. As such, the age structure of the population forms a key parameter. Accurate age determination in bivalve shells is not always straightforward due to the difficulty of interpreting externally visible growth lines. In the present paper, we aimed at validating the seasonality in growth line formation using visual techniques in combination with stable oxygen and carbon isotope analyses (δ<sup>18</sup>O and δ<sup>13</sup>C). <br><br> High δ<sup>18</sup>O values in the shell coincided with growth marks on the external surface of the valve and in acetate peels of the shell's cross section. Most shell δ<sup>18</sup>O samples were assigned to the months from June to September. From November to March no samples were retrieved, indicating that the shell did not grow. The lowest reconstructed temperature (6.3 °C) suggests that ~ 6 °C may be the threshold temperature for growth. Nevertheless, most of the reconstructed values fell above 14.5 °C, indicating that growth occurred mainly in the summer at relatively high temperatures. Shell δ<sup>13</sup>C profiles followed a more or less seasonal cycle, but no direct relationship could be made between δ<sup>13</sup>C values and annual growth lines. Although counting external annual growth lines led to a correct estimation of age and consequently of growth rates, we recommend analysing acetate peels of cross sections to support the distinction between annual lines and disturbance lines.