Anthropogenic activity has led to significant emissions of greenhouse gas (GHG), which is thought to play important roles in global climate changes. It remains unclear about the kinetics of GHG emissions, including carbon dioxide (CO<sub>2</sub>), methane (CH<sub>4</sub>) and nitrous Oxide (N<sub>2</sub>O) from the Three Gorges Reservoir (TGR) of China, which was formed after the construction of the famous Three Gorges Dam. Here we report monthly measurements for one year of the fluxes of these gases at multiple sites within the TGR region, including three major tributaries, six mainstream sites, two downstream sites and one upstream site. The tributary areas have lower CO<sub>2</sub> fluxes than the main storage; CH<sub>4</sub> fluxes in the tributaries and upper reach mainstream sites are relative higher. Overall, TGR showed significantly lower CH<sub>4</sub> emission rates than most new reservoirs in temperate and tropical regions. We attribute this to the well-oxygenated deep water and high water velocities that may facilitate the consumption of CH<sub>4</sub>. TGR's CO<sub>2</sub> fluxes were lower than most tropical reservoirs and higher than most temperate systems. This could be explained by the high load of labile soil carbon delivered through erosion to the Yangtze River. Compared to fossil-fuelled power plants of equivalent power output, TGR is a very small GHG emitter – annual CO<sub>2</sub>-equivalent emissions are approximately 1.7% of that of a coal-fired generating plant of comparable power output.