Biogeosciences www.biogeosciences.net 1726-4170 1726-4189 7 1 2010 10.5194/bg-7-333-2010 http://www.biogeosciences.net/7/333/2010/ http://www.biogeosciences.net/7/333/2010/bg-7-333-2010.html http://www.biogeosciences.net/7/333/2010/bg-7-333-2010.pdf 333 341 2010-01-28 A kinetic analysis of leaf uptake of COS and its relation to transpiration, photosynthesis and carbon isotope fractionation U. Seibt ulli@atmos.ucla.edu J. Kesselmeier L. Sandoval-Soto U. Kuhn J. A. Berry Université Pierre et Marie Curie Paris 6, UMR BioEmco, Campus ParisAgroTech, 78850 Thiverval-Grignon, France Max Planck Institute for Chemistry, Biogeochemistry Dept., Joh.-J.-Becher-Weg 27, 55128 Mainz, Germany Carnegie Institution for Science, Department of Global Ecology, 260 Panama St., Stanford, CA 94305-1297, USA now at: Fachhochschule Nordwestschweiz, Hochschule für Life Sciences, Institut für Ecopreneurship, Gründenstraße 40, 4132 Muttenz, Switzerland now at: Agroscope Reckenholz-Tänikon Research Station ART, Reckenholzstraße 191, 8046 Zürich, Switzerland Carbonyl sulfide (COS) is an atmospheric trace gas that holds great promise for studies of terrestrial carbon and water exchange. In leaves, COS follows the same pathway as CO₂ during photosynthesis. Both gases are taken up in enzyme reactions, making COS and CO₂ uptake closely coupled at the leaf scale. The biological background of leaf COS uptake is a hydrolysis reaction catalyzed by the enzyme carbonic anhydrase. Based on this, we derive and test a simple kinetic model of leaf COS uptake, and relate COS to CO₂ and water fluxes at the leaf scale. The equation was found to predict realistic leaf COS fluxes compared to observations from field and laboratory chambers. We confirm that COS uptake at the leaf level is directly linked to stomatal conductance. As a consequence, the ratio of normalized uptake rates (uptake rates divided by ambient mole fraction) for leaf COS and CO₂ fluxes can provide an estimate of <i>C_i</i>/<i>C_a</i>, the ratio of intercellular to atmospheric CO₂, an important plant gas exchange parameter that cannot be measured directly. The majority of published normalized COS to CO₂ uptake ratios for leaf studies on a variety of species fall in the range of 1.5 to 4, corresponding to <i>C_i</i>/<i>C_a</i> ratios of 0.5 to 0.8. 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