Biogeosciences www.biogeosciences.net 1726-4170 1726-4189 6 4 2009 10.5194/bg-6-705-2009 http://www.biogeosciences.net/6/705/2009/ http://www.biogeosciences.net/6/705/2009/bg-6-705-2009.html http://www.biogeosciences.net/6/705/2009/bg-6-705-2009.pdf 705 720 2009-04-28 Bottom up effects on bacterioplankton growth and composition during summer-autumn transition in the open NW Mediterranean Sea F. Van Wambeke france.van-wambeke@univmed.fr J.-F. Ghiglione J. Nedoma G. Mével P. Raimbault Laboratoire de Microbiologie, Géochimie et Ecologie Marines, CNRS, Université de la Méditerranée, Centre d'Océanologie de Marseille, UMR6117, Campus de Luminy, case 901, 13 288 Marseille Cedex 09, France CNRS, UMR7621, Laboratoire d'Océanographie Biologique de Banyuls, Avenue Fontaulé, BP 44, 66 650 Banyuls-sur-Mer, France UPMC Univ Paris 06, UMR7621, Laboratoire ARAGO, Avenue Fontaulé, BP 44, 66 650 Banyuls-sur-Mer, France Biological Centre of the Academy of Sciences of the Czech Republic, Hydrobiological Institute, Na sádkách 7, 37005 České Budějovice, Czech Republic CNRS, UMR7144, Equipe de Chimie Marine, Station Biologique de Roscoff, 29 682 Roscoff, France UPMC Univ Paris 06, Equipe de Chimie Marine, Station Biologique de Roscoff, 29 682 Roscoff, France We examined the vertical and temporal dynamics of nutrients, ectoenzymatic activities under late summer-fall transition period (September–October 2004) in NW Mediterranean Sea in relation to temporal change in factors limiting bacterial production. The depth of the mixed layer (12.8±5.3 m) was extremely stable until the onset of the destratification period after 11 October, creating a zone where diffusion of nutrient from the much deeper phosphacline (69±12 m) and nitracline (50±8 m) was probably strongly limited. However after 1st October, a shallowing of nutriclines occured, particularly marked for nitracline. Hence, the nitrate to phosphate ratio within the mixed layer, although submitted to a high short term variability, shifted the last week of the cruise from 1.1±1.2 to 4.6±3.8, and nitrate increased by a factor 2 (0.092±0.049 μM). A corresponding switch from more than one limitation (PN) to P-only limitation of bacterial production was observed during the month as detected by enrichment bioassays. 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