Biogeosciences www.biogeosciences.net 1726-4170 1726-4189 6 2 2009 10.5194/bg-6-171-2009 http://www.biogeosciences.net/6/171/2009/ http://www.biogeosciences.net/6/171/2009/bg-6-171-2009.html http://www.biogeosciences.net/6/171/2009/bg-6-171-2009.pdf 171 179 2009-02-11 Temporal variability in bioassays of the stomatal ammonia compensation point in relation to plant and soil nitrogen parameters in intensively managed grassland M. Mattsson B. Herrmann M. David B. Loubet M. Riedo M. R. Theobald M. A. Sutton D. Bruhn A. Neftel J. K. Schjoerring jks@life.ku.dk Plant and Soil Science Laboratory, University of Copenhagen, Faculty of Life Sciences, Thorvaldsensvej 40, 1871 Frederiksberg C, Copenhagen, Denmark Agroscope Reckenholz-Tänikon Rsearch Station ART, Reckenholzstrasse 191, 8046 Zürich, Switzerland Institut National de la Recherche Agronomique (INRA), UMR Environnement et Grandes Cultures, Thiverval-Grignon, France Natural Environmental Research Council, Centre for Ecology and Hydrology, Edinburgh Research Station, Penicuik EH260QB, Midlothian, Scotland now at: Section for Economy and Technology, Halmstad University, 30118 Halmstad, Sweden The exchange of ammonia between crop canopies and the atmosphere depends on a range of plant parameters and climatic conditions. However, little is known about effects of management factors. We have here investigated the stomatal ammonia compensation point in response to cutting and fertilization of a grass sward dominated by <i>Lolium perenne</i>. Tall grass had a very low NH₃ compensation point (around 1 nmol mol^&minus;1), reflecting the fact that leaf nitrogen (N) concentration was very low. During re-growth after cutting, leaf tissue concentrations of NO₃^&minus;, NH₄⁺, soluble N and total N increased along with apoplastic NH₄⁺ concentrations. In contrast, apoplastic pH decreased resulting in largely unaltered NH₃ compensation points. Nitrogen fertilization one week after cutting caused the apoplastic NH₄⁺ concentration of the newly emerging leaves to increase dramatically. The NH₃ compensation point peaked between 15 and 25 nmol mol^&minus;1 the day after the fertiliser was applied and thereafter decreased over the following 10 days until reaching the same level as before fertilisation. Ammonium concentrations in leaf apoplast, bulk tissue and litter were positively correlated (P=0.001) throughout the experimental period. Bulk tissue NH₄⁺ concentrations, total plant N and soil NH₄⁺ concentrations also showed a positive correlation. A very high potential for NH₃ emission was shown by the plant litter. Bakken, A. K., Macduff, J. H., and Collison, M.: Dynamics of nitrogen remobilization in defoliated \textitPhleum pratense and \textitFestuca pratensis under short and long photoperiods, Physiol. Plant., 103, 426–436, 1998. Bloom, A. J. and Chapin, F. S.: Differences in steady-state net ammonium and nitrate influx by cold- and warm adapted barley varieties, Plant Physiol., 68, 1064–1067, 1981. Clarkson, D. T., Hopper, M. J., and Jones, L. H. P.: The effect of root temperature on the uptake of nitrogen and the relative size of the root system in \textitLolium perenne. I. Solutions containing both NH$_4^+$ and NO$_3^-$, Plant Cell Environ., 9, 535–545, 1986. David, M., Roche, R., Mattsson M., Schjoerring, J. K., Sutton, M. A., Daemmgen, U., and Cellier, P.: Analysis of ammonia fluxes with intensively managed grassland using dynamic chambers II. The effect of management options, Biogeosciences Discuss., (in press), 2009. Farquhar, G. D., Firth, P. M., Wetselaar, R., and Weir, B.: On the gaseous exchange of ammonia between leaves and the environment. Determination of the ammonia compensation point, Plant Physiol., 66, 710–714, 1980. Hanstein, S., Mattsson, M., Jaeger, H.-J., and Schjoerring, J. K.: Uptake and utilization of atmospheric ammonia in three native \textitPoaceae species: Leaf conductances, composition of apoplastic solution and interactions with nitrogen supply, New Phytol., 141, 71–83, 1999. Herrmann, B., Jones, S. K., Fuhrer, J., Feller, U., and Neftel, A.: N budget and NH₃ exchange of a grass/clover crop at two levels of N application, Plant and Soil, 235, 243–252, 2001. Herrmann, B., Mattsson, M., Jones, S. K., Cellier, P., Milford, C., Sutton, M. A., Schjoerring, J. K., and Neftel, A.: Vertical structure and diurnal variability of ammonia exchange potential within an intensively managed grass canopy, Biogeosciences, 6, 15–23, 2009. Hill, P. W., Raven, J. A., and Sutton, M. A.: Leaf age-related differences in apoplastic NH$_4^+$ concentration, pH and the NH₃ compensation point for a wild perennial, J. Exp. Bot., 53, 277–286, 2002. Horvath, L., Astalos, M., Fuhrer, E., Meszaros, R., and Weidinger, T.: Measurement of ammonia exchange over grassland in the Hungarian Great Plain, Agric. Forest Meteorol., 130, 282–298, 2005. Husted, S. and Schjoerring, J. K.: Apoplastic pH and ammonium concentration in leaves of \textitBrassica napus L., Plant Physiol., 109, 1453–1460, 1995. Husted, S. and Schjoerring, J. K.: Ammonia flux between oilseed rape plants and the atmosphere in response to changes in leaf temperature, light intensity, and air humidity, Plant Physiol., 112, 67–74, 1996. Husted, S., Mattsson, M, and Schjoerring, J. K.: Ammonia compensation points in two cultivars of \textitHordeum vulgare L. during vegetative and generative growth, Plant, Cell Environ., 19, 1299–1306, 1996. Husted, S., Hebbern, C. A., Mattsson, M., and Schjoerring, J. K.: Determination of ammonium, low molecular weight amines and amides in plant tissue, Physiol. Plant., 109, 167–179, 2000a. Husted, S., Schjoerring, J. K., Nielsen, K. H. Nemitz, E., and Sutton, M. A.: Stomatal compensation points for ammonia in oilseed rape plants under field conditions, Agr. Forest Meteorol., 105, 371–383, 2000b. Loubet, B., Milford, C., Hill, P. W., Tang, Y. S., Cellier, P., and Sutton, M. S.: Seasonal variability of apoplastic NH$_4^+$ and pH in an intensively managed grassland, Plant and Soil, 238, 97–110, 2002. Mattsson, M. and Schjoerring, J. K.: Ammonia emission from young barley plant: influence of N-source, light/dark cycles and inhibition of glutamine synthetase, J. Exp. Bot., 47, 477–484, 1996. Mattsson, M., Häusler, R. E., Leegood, R. C., Lea, P. J., and Schjoerring, J. K.: Leaf-atmosphere ammonia exchange in barley mutants with reduced activities of glutamine synthetase, Plant Physiol., 114, 1307–1312, 1997. Mattsson, M., Husted, S., and Schjoerring, J. K.: Influence of nitrogen nutrition and metabolism on ammonia volatilization in plants, Nutr. Cycl. Agroecosys., 51, 35–40, 1998. Mattsson, M. and Schjoerring, J. K.: Dynamic and steady state responses of inorganic nitrogen pools and NH₃ exchange in leaves of \textitLolium perenne and \textitBromus erectus to changes in root supply, Plant Physiol, 128, 742–750, 2002. Mattsson, M. and Schjoerring, J. K.: Senescence-induced changes in apoplastic and bulk tissue ammonia concentrations of rye-grass leaves, New Phytol., 160, 489–499, 2003. Mattsson, M., Herrmann, B., Jones, S., Borella, S., Dorsey, J., and Schjoerring, J. K.: Contribution of different grass species to NH₃ exchange between plants and the atmosphere in intensively managed grassland, Biogeosciences, 6, 59–66, 2009. Milford, C., Theobald, M. R., Nemitz, E. N., Hargreaves, K. J., Horvath, L., Raso, J., Daemmgen, U., Neftel, A., Jones, S., Hensen, A., Loubet, B., and Sutton, M. A.: Ammonia fluxes in relation to cutting and fertilization of intensively managed grassland derived from an inter-comparison of gradient measurements, Biogeosciences Discuss., 5, 4699–4744, 2008. Nemitz, E., Sutton, M. A., Gut, A., San José, R., Husted, S., and Schjoerring, J. K.: Sources and sinks of ammonia within an oilseed rape canopy, Agric. Forest Meteorol., 105, 385–404, 2000. Ourry, A., Boucard, J., and Salette, J.: Nitrogen remobilisation from stubble and roots during re-growth of defoliated ryegrass, J. Exp. Bot., 39, 803–809, 1988. Ourry, A., Gonzales, B., and Boucaud, J.: Osmoregulation and role of nitrate during regrowth after cutting of ryegrass (\textitLolium perenne), Physiol. Plant., 76, 177–182, 1989. Riedo, M., Milford, C., Schmid, M., and Sutton, M. A.: Coupling soil-plant-atmosphere exchange of ammonia with ecosystem functioning in grasslands, Ecol. Model., 158, 83–110, 2002.. Schjoerring, J. K. and Mattsson, M.: Quantification of ammonia exchange between agricultural cropland and the atmosphere: Measurements over two complete growth cycles of oilseed rape, wheat, barley and pea, Plant Soil, 228, 105–115, 2001. Sutton, M. A., Milford, C., Nemitz, E., Theobald, M. R., Hill, P. W., Fowler, D., Schjoerring, J. K., Mattsson, M., Nielsen, K. H., Husted, S., Erisman, J. W., Otjes, R., Hensen, A., Cellier, P., Loubet, B., David, M., Genermont, S., Neftel, A., Blatter, A., Hermann, B., Jones, S. K., Horvath, L., Führer, E., Mantzanas, C., Koukoura, K., Gallagher, M., Williams, P., and Riedo, M.: Biosphere-atmosphere interactions of ammonia with grasslands: experimental strategy and results from a new European initiative, Plant Soil, 228, 131–135, 2001. Schäufele, R. and Schnyder, H.: Carbon and nitrogen deposition in expanding tissue elements of perennial ryegrass (\textitLolium perenne L) leaves during non-steady growth after defoliation, Plant. Cell Environ., 24, 407–417, 2001. Sommer, S. G., Schjoerring, J. K., and Denmead, O. T.: Ammonia emission from mineral fertilizers and fertilized crops, Adv. Agron., 82, 557–622, 2004. Sutton, M. A., Nemitz, E., Theobald, M. R., Milford, C., Dorsey, J. R., Gallagher, M. W., Hensen, A., Jongejan, P. A. C., Erisman, J.,W., Mattsson, M., Schjoerring, J. K., Cellier, P., Loubet, B., Roche, R., Neftel, A., Herrmann, B., Jones, S., Lehman, B. E., Horvath, L., Weidinger, T., Rajkai, K., Burkhardt, J., Löpmeier, F. J., and Daemmgen U.: Dynamics of ammonia exchange with cut grassland: Strategy and implementation of the GRAMINAE Integrated Experiment, Biogeosciences Discuss., 5, 3347–3407, 2008. Trebs, I., Lara, L. L., Zeri, L. M. M., Gatti, L. V., Artaxo, P., Dlugi, R., Slanina, J., Andreae, M. O., and Meixner, F. X.: Dry and wet deposition of inorganic nitrogen compounds to a tropical pasture site (Rondonia, Brazil), Atmos. Chem. Phys., 6, 447–469, 2006. van Hove, L. W. A., Heeres, P., and Bossen, M. E.: The annual variation in stomatal ammonia compensation point of rye grass (\textitLolium perenne L.) leaves in an intensively managed grassland, Atmos. Environ., 36, 2965–2977, 2002. Whitehead, D. C.: Grasses: Uptake of nitrogen and effects on morphology and physiology. In: DC Whitehead, Ed. Grassland nitrogen. CAB International, UK 16–34, 1995. Wichink Kruit, R. J., van Pul, W. A. J., Otjes, R. P., Hofschreuder, P., Jacobs, A. F. G., and Holtslag, A. A. M.: Ammonia fluxes and derived canopy compensation points over non-fertilized agricultural grassland in The Netherlands using the new gradient ammonia – high accuracy – monitor (GRAHAM), Atmos. Environ., 41, 1275–1287, 2007.