Biogeosciences www.biogeosciences.net 1726-4170 1726-4189 5 3 2008 10.5194/bg-5-833-2008 http://www.biogeosciences.net/5/833/2008/ http://www.biogeosciences.net/5/833/2008/bg-5-833-2008.html http://www.biogeosciences.net/5/833/2008/bg-5-833-2008.pdf 833 845 2008-05-19 Factors limiting heterotrophic bacterial production in the southern Pacific Ocean F. Van Wambeke france.van-wambeke@univmed.fr S. Bonnet T. Moutin P. Raimbault G. Alarcón C. Guieu Laboratoire de Microbiologie, Géochimie et Ecologie Marines (LMGEM), CNRS, UMR 6117, Université de la Méditerranée, Campus de Luminy – Case 901, 13 288 Marseille cedex 9, France CNRS, Laboratoire d'océanographie de Villefranche, 06 230 Villefranche-sur-Mer, France; Université Pierre et Marie Curie - Paris6, Laboratoire d'océanographie de Villefranche, 06 230 Villefranche-sur-Mer, France Laboratoire d'Océanographie et de Biogéochimie (LOB), CNRS, UMR 6535, Université de la Méditerranée, Campus de Luminy – Case 901, 13 288 Marseille cedex 9, France Department of Oceanography & Center for Oceanographic Research in the eastern South Pacific, University of Concepcion, Casilia 160-C, Concepcion, Chile The role of potential factors limiting bacterial growth was investigated along vertical and longitudinal gradients across the South Eastern Pacific Gyre. The effects of glucose, nitrate, ammonium and phosphate additions on heterotrophic bacterial production (using leucine technique) were studied in parallel in unfiltered seawater samples incubated under natural daily irradiance. The enrichments realized on the subsurface showed three types of responses. From 141° W (Marquesas plateau) to approx 125° W, bacteria were not bottom-up controlled, as confirmed by the huge potential of growth in non-enriched seawater (median of enhancement factor×39 in 24 h). Within the Gyre (125° W–95° W), nitrogen alone stimulated leucine incorporation rates (median×4.2), but rapidly labile carbon (glucose) became a second limiting factor (median×37) when the two elements were added. Finally from the border of the gyre to the Chilean upwelling (95° W–73° W), labile carbon was the only factor stimulating heterotrophic bacterial production. 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