Biogeosciences www.biogeosciences.net 1726-4170 1726-4189 5 3 2008 10.5194/bg-5-739-2008 http://www.biogeosciences.net/5/739/2008/ http://www.biogeosciences.net/5/739/2008/bg-5-739-2008.html http://www.biogeosciences.net/5/739/2008/bg-5-739-2008.pdf 739 748 2008-05-07 Effects of increased atmospheric CO2 on small and intermediate sized osmotrophs during a nutrient induced phytoplankton bloom A. I. Paulino J. K. Egge A. Larsen aud.larsen@bio.uib.no Department of Biology, Microbiology, University of Bergen, P. Box 7800, N-5020 Bergen, Norway We report the transient population dynamic response of the osmotrophic community initiated by a nutrient pulse in mesocosms exposed to different pCO2 levels. Differences in phytoplankton and heterotrophic bacteria abundances associated with the CO2 treatment are also described. Coastal seawater was enclosed in floating mesocosms (27 m3) and nutrients were supplied initially in order to stimulate growth of microbial organisms, including the coccolitophorid Emiliania huxleyi. The mesocosms were modified to achieve 350 μatm (1×CO2), 700 μatm (2×CO2) and 1050 μatm (3×CO2) CO2 pressure. The temporal dynamics was related to nutrient conditions in the enclosures. Numerically small osmotrophs (picoeukaryotes and Synechoccocus sp.) dominated initially and towards the end of the experiment, whereas intermediate sized osmotrophs bloomed as the initial bloom of small sized osmotrophs ceased. Maximum concentrations of E. huxleyi were approximately 4.6×103 cells ml−1 whereas other intermediate sized osmotrophs reached approximately twice as high concentrations. The osmotrophic succession pattern did not change, and neither were we able to detect differences with regard to presence or absence of specific osmotrophic taxa as a consequence of altered pCO2. 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