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	<journal>
		<journal_title>Biogeosciences</journal_title>
		<journal_url>www.biogeosciences.net</journal_url>
		<issn>1726-4170</issn>
		<eissn>1726-4189</eissn>
		<volume_number>5</volume_number>
		<issue_number>2</issue_number>
		<publication_year>2008</publication_year>
	</journal>
	<doi>10.5194/bg-5-523-2008</doi>
	<article_url>http://www.biogeosciences.net/5/523/2008/</article_url>
	<abstract_html>http://www.biogeosciences.net/5/523/2008/bg-5-523-2008.html</abstract_html>
	<fulltext_pdf>http://www.biogeosciences.net/5/523/2008/bg-5-523-2008.pdf</fulltext_pdf>
	<start_page>523</start_page>
	<end_page>533</end_page>
	<publication_date>2008-04-09</publication_date>
	<article_title content_type="html">Response of marine viral populations to a nutrient induced phytoplankton bloom at different pCO&lt;sub&gt;2&lt;/sub&gt; levels</article_title>
	<authors>
		<author numeration="1" affiliations="1">
			<name>J. B. Larsen</name>
		</author>
		<author numeration="2" affiliations="1">
			<name>A. Larsen</name>
			<email>aud.larsen@bio.uib.no</email>
		</author>
		<author numeration="3" affiliations="1">
			<name>R. Thyrhaug</name>
		</author>
		<author numeration="4" affiliations="1">
			<name>G. Bratbak</name>
		</author>
		<author numeration="5" affiliations="1">
			<name>R.-A. Sandaa</name>
		</author>
	</authors>
	<affiliations>
		<affiliation numeration="1" content_type="html">Department of Biology, Jahnebakken 5, University of Bergen, P. Box 7800, 5020 Bergen, Norway</affiliation>
	</affiliations>
	<abstract content_type="html">During the PeECE III mesocosm experiment in 2005 we investigated how the
virioplankton community responded to increased levels of nutrients (N and P)
and CO&lt;sub&gt;2&lt;/sub&gt;. We applied a combination of flow cytometry, Pulsed Field Gel
Electrophoresis and degenerate PCR primers to categorize and quantify
individual viral populations, and to investigate their temporal dynamics.
Species specific and degenerate primers enabled us to identify two specific
large dsDNA viruses, EhV and CeV, infecting the haptophytes &lt;i&gt;Emiliania huxleyi&lt;/i&gt; and
&lt;i&gt;Crysochromulina ericina&lt;/i&gt;, respectively. Some of the viral populations detected and enumerated by
flow cytometry did not respond to altered CO&lt;sub&gt;2&lt;/sub&gt;-levels, but the abundance
of EhV and an unidentified dsDNA virus decreased with increasing CO&lt;sub&gt;2&lt;/sub&gt;
levels. Our results thus indicate that CO&lt;sub&gt;2&lt;/sub&gt; conditions, or the related
change in pH, may affect the marine pelagic food web at the viral level. Our
results also demonstrate that in order to unravel ecological problems as how
CO&lt;sub&gt;2&lt;/sub&gt; and nutrient levels affect the relationship between marine algal
viruses and their hosts, we need to continue the effort to develop molecular
markers used to identify both hosts and viruses.</abstract>
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</article>

