Biogeosciences www.biogeosciences.net 1726-4170 1726-4189 5 5 2008 10.5194/bg-5-1425-2008 http://www.biogeosciences.net/5/1425/2008/ http://www.biogeosciences.net/5/1425/2008/bg-5-1425-2008.html http://www.biogeosciences.net/5/1425/2008/bg-5-1425-2008.pdf 1425 1435 2008-10-21 Heterogeneous ice nucleation activity of bacteria: new laboratory experiments at simulated cloud conditions O. Möhler D. G. Georgakopoulos C. E. Morris S. Benz V. Ebert S. Hunsmann H. Saathoff M. Schnaiter R. Wagner Institute for Meteorology and Climate Research (IMK-AAF), Forschungszentrum Karlsruhe, Germany Department of Agricultural Biotechnology, Agricultural University of Athens, Greece INRA, Unité de Pathologie Végétale UR407, Montfavet, France Institute for Physical Chemistry, University of Heidelberg, Germany The ice nucleation activities of five different Pseudomonas syringae, Pseudomonas viridiflava and Erwinia herbicola bacterial species and of Snomax™ were investigated in the temperature range between −5 and −15°C. Water suspensions of these bacteria were directly sprayed into the cloud chamber of the AIDA facility of Forschungszentrum Karlsruhe at a temperature of −5.7°C. At this temperature, about 1% of the Snomax™ cells induced immersion freezing of the spray droplets before the droplets evaporated in the cloud chamber. The living cells didn't induce any detectable immersion freezing in the spray droplets at −5.7°C. After evaporation of the spray droplets the bacterial cells remained as aerosol particles in the cloud chamber and were exposed to typical cloud formation conditions in experiments with expansion cooling to about −11°C. During these experiments, the bacterial cells first acted as cloud condensation nuclei to form cloud droplets. Then, only a minor fraction of the cells acted as heterogeneous ice nuclei either in the condensation or the immersion mode. The results indicate that the bacteria investigated in the present study are mainly ice active in the temperature range between −7 and −11°C with an ice nucleation (IN) active fraction of the order of 10−4. In agreement to previous literature results, the ice nucleation efficiency of Snomax™ cells was much larger with an IN active fraction of 0.2 at temperatures around −8°C. Amato, P., Parazols, M., Sancelme, M., Laj, P., Mailhot, G., and Delort, A M.: Microorganisms isolated from the water phase of tropospheric clouds at the Puy de Dome: major groups and growth abilities at low temperatures, FEMS Microbiol. Ecol., 59, 242–254, 2007. Bauer, H., Giebl, H., Hitzenberger, R., Kasper-Giebl, A., Reischl, G., Zibuschka, F., and Puxbaum, H.: Airborne bacteria as cloud condensation nuclei, J. Geophys. Res.-Atmos., 108, 4658, doi:10.1029/2003JD003545, 2003. Benz, S., Megahed, K., Möhler, O., Saathoff, H., Wagner, R., and Schurath, U.: T-dependent rate measurements of homogeneous ice nucleation in cloud droplets using a large atmospheric simulation chamber, J. Photoch. Photobio. A, 176, 208–217, 2005. Constantinidou, H A., Hirano, S S., Baker, L S., and Upper, C D.: Atmospheric Dispersal of Ice Nucleation-Active Bacteria – the Role of Rain, Phytopathology, 80, 934–937, 1990. Ebert, V., Teichert, H., Giesemann, C., Saathoff, H., and Schurath, U.: Fiber-coupled in-situ laser absorption spectrometer for the selective detection of water vapour traces down to the ppb-level, Tech. Mess., 72, 23–30, 2005. Franc, G D. and DeMott, P J.: Cloud activation characteristics of airborne Erwinia carotovora cells, J. Appl. Meteorol., 37, 1293–1300, 1998. Jayaweera, K. and Flanagan, P.: Investigations on biogenic ice nuclei in the arctic atmosphere, Geophys. Res. Lett., 9, 94–97, 1982. King, E O., Ward, M N., and Raney, D T.: Two simple media for the demonstration of pyocyanin and fluorescein, J. Lab. Clin. Med., 44, 301–307, 1954. Lee, R E., Warren, G J., and Gusta, L V.: Biological Ice Nuclation and Its Applications, APS Press, The American Phytopathological Society, St. Paul, Minnesota, 1995. Levin, Z. and Yankofsky, S A.: Contact Versus Immersion Freezing of Freely Suspended Droplets by Bacterial Ice Nuclei, J. Appl. Meteorol., 22, 1964–1966, 1983. Lindemann, J., Constantinidou, H A., Barchet, W R., and Upper, C D.: Plants as a source of airborne bacteria, including ice nucleation active bacteria, Appl. Environ. Microb., 44, 1059–1063, 1982. Lindow, S E.: Population dynamics of epiphytic ice nucleation active bacteria on frost sensiteive plants and frost control by means of antagonistic bacteria, in: Plant Cold Hardiness and Freezing Stress, edited by: Sakai, A. and Li, P H., vol 2, Academic Press, New York, 1982. Maki, L R., Galyan, E L., Chang-Chien, M M., and Caldwell, D R.: Ice nucleation induced by Pseudomonas syringae, Appl. Environ. Microb., 28, 456–459, 1974. Mangold, A., Wagner, R., Saathoff, H., Schurath, U., Giesemann, C., Ebert, V., Kramer, M., and Möhler, O.: Experimental investigation of ice nucleation by different types of aerosols in the aerosol chamber AIDA: implications to microphysics of cirrus clouds, Meteorol. Z., 14, 485–497, 2005. Möhler, O., Büttner, S., Linke, C., Schnaiter, M., Saathoff, H., Stetzer, O., Wagner, R., Krämer, M., Mangold, A., Ebert, V., and Schurath, U.: Effect of sulfuric acid coating on heterogeneous ice nucleation by soot aerosol particles, Journal of Geophysical Research-Atmospheres, 110, D11 210, doi:10.1029/2004JD005169, 2005. Möhler, O., Field, P R., Connolly, P., Benz, S., Saathoff, H., Schnaiter, M., Wagner, R., Cotton, R., Krämer, M., Mangold, A., and Heymsfield, A J.: Efficiency of the deposition mode ice nucleation on mineral dust particles, Atmos. Chem. Phys., 6, 3007–3021, 2006. Möhler, O., DeMott, P J., Vali, G., and Levin, Z.: Microbiology and atmospheric processes: the role of biological particles in cloud physics, Biogeosciences, 4, 1059–1071, 2007. Morris, C E., Wen, A.-M., Xu, X.-H., and Di, Y.-B.: Ice nucleation active bacteria on Chinese cabbage in northern China : populations dynamics and characteristics and their possible role in storage decay, Phytopathology, 82, 739–746, 1992. Morris, C E., Georgakopoulos, D G., and Sands, D C.: Ice nucleation active bacteria and their potential role in precipitation, J. Phys. IV, 121, 87–103, 2004. Morris, C E., Sands, D C., Vinatzer, B A., Glaux, C., Guilbaud, C., Buffière, A., Yan, S., Dominguez, H., and Thompson, B M.: The life history of the plant pathogen Pseudomonas syringae is linked to the water cycle, The ISME Journal, 2, 321–334, doi:10.1038/ismej.2007.113, 2008. Sands, D C., Langhans, V E., Scharen, A L., and Smet, G.: The association between bacteria and rain and possible resultant meteorological implications, Quarterly Journal of Hungarian Meteorological Service, 86, 148–152, 1982. Vali, G., Christensen, M., Fresh, R W., Galyan, L R., Maki, L R., and Schnell, R C.: Biogenic Ice Nuclei. Part II: Bacterial Sources, J. Atmos. Sci., 33, 1565-1570, doi:10.1175/1520–0469(1976)033, 1976. Wagner, R., Benz, S., Möhler, O., Saathoff, H., and Schurath, U.: Probing ice clouds by broadband mid-infrared extinction spectroscopy: case studies from ice nucleation experiments in the AIDA aerosol and cloud chamber, Atmos. Chem. Phys., 6, 4775–4800, 2006. Ward, P J. and DeMott, P J.: Preliminary experimental evaluation of Snomax, Pseudomonas syringae, as an artificial ice nucleus for wheather modification, Journal of Weather Modification, 21, 9–13, 1989. Wood, S E., Baker, M B., and Swanson, B D.: Instrument for studies of homogeneous and heterogeneous ice nucleation in free-falling supercooled water droplets, Rev. Sci. Instrum., 73, 3988–3996, 2002. Yankofsky, S A., Levin, Z., Bertold, T., and Sandlerman, N.: Some basic characteristics of bacterial freezing nuclei, J. Appl. Meteorol., 20, 1013–1019, 1981.