Biogeosciences www.biogeosciences.net 1726-4170 1726-4189 4 1 2007 10.5194/bg-4-1-2007 http://www.biogeosciences.net/4/1/2007/ http://www.biogeosciences.net/4/1/2007/bg-4-1-2007.html http://www.biogeosciences.net/4/1/2007/bg-4-1-2007.pdf 1 9 2007-01-10 Culturable bacteria in Himalayan glacial ice in response to atmospheric circulation S. Zhang S. Hou shugui@lzb.ac.cn X. Ma D. Qin T. Chen Laboratory of Cryosphere and Environment, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou 730000, China School of Life Science, Lanzhou University, Lanzhou 730000, China Only recently has specific attention been given to culturable bacteria in Tibetan glaciers, but their relation to atmospheric circulation is less understood yet. Here we present the results of culturable bacteria preserved in an ice core drilled from the East Rongbuk (ER) glacier, Himalayas. The average concentrations of culturable bacteria are 5.0, 0.8, 0.1 and 0.7 CFU mL^−1 for the glacier ice deposited during the premonsoon, monsoon, postmonsoon and winter seasons, respectively. The high concentration of culturable bacteria in ER glacier deposited during the premonsoon season is attributed to the transportation of continental dust stirred up by the frequent dust storms during spring. This is also confirmed by the spatial distribution of culturable bacteria in Tibetan glaciers. Continental dust originated from the Northwest China accounts for the high abundance of culturable bacteria in the northern Tibetan Plateau, while monsoon moisture exerts great influence on culturable bacteria with low abundance in the southern plateau. The numbers of representatives with different ARDRA patterns from RFLP analysis are 10, 15, 1 and 2 for the glacial ice deposited during the premonsoon, monsoon, postmonsoon and winter seasons, respectively, suggesting that culturable bacteria deposited in ER glacier during monsoon season are more diverse than that deposited during the other seasons, possibly due to their derivation from both marine air masses and local or regional continental sources, while culturable bacteria deposited during the other seasons are from only one possible origin that is transported by westerlies. Our results show the first report of seasonal variations of abundance and species diversity of culturable bacteria recovered from glacial ice in the Himalayas, and we suggest that microorganisms in Himalayan ice might provide a potential new proxy for the reconstruction of atmospheric circulation. Abyzov, S. S., Mitskevich, I. N., and Poglazova, M. N.: Micro?ora of the deep glacier horizons of central Antarctica, Microbiology (Moscow), 67, 66–73, 1998. Altschul, S. F., Gish, W., Miller, W., Myers, E. W., and Lipman, D. J.: Basic local alignment search tool, J. Mol. Biol., 215, 403–410, 1990. Brinkmeyer, R., Knittel, K., Jurgens, J., Weyland, H., Amann, R., and Helmke, E.: Diversity and Structure of Bacterial Communities in Arctic versus Antarctic Pack Ice, Appl. Environ. Microbiol., 69, 6610–6619, 2003. Bryson, R. A.: Air stream climatology of Asia. In: Proceedings of the International Symposium on the Qinghai-Xizang Plateau and Mountain Meteorology, American Meteorological Society, Boston, MA, 604-617, 1986. Castello, J. D., Rogers, S. O., Starmer, W. T., Catranis, C. M., Ma, L., Bachand, G. D., Zhao, Y., and Smith, J. E.: Detection of tomato mosaic tobamovirus RNA in ancient glacial ice, Polar Biol., 22, 207–212, 1999. Chin, K. J., Hahn, D., Hengstmann, U., Liesack, W., and Janssen, P. H.: Characterization and identification of numerically abundant culturable bacteria from the anoxic bulk soil of rice paddy microcosms, Appl. Environ. Microbiol., 65, 5042–5049, 1999. Christner, B. C., Mosley-Thompson, E., Thompson, L. G., Zagorodnov, V., Sandman, K., and Reeve, J. N.: Recovery and identification of viable bacteria immured in glacial ice, Icarus, 144, 479–485, 2000. Christner, B. C., Mosley-Thompson, E., Thompson, L. G., and Reeve, J. N.: Isolation of bacteria and 16S rRNAs from Lake Vostok accretion ice, Environ. Microbiol., 3, 570–577, 2001. Christner, B. C.: Detection, recovery, isolation, and characterization of bacteria in glacial ice and Lake Vostok accretion ice. Ph. D. theis. The Ohio State University, Columbus, 2002. Dai, X., Wang, Y. N., Wang, B. J., Liu, S. J., and Zhou, Y. G.: Planomicrobium chinense sp. nov., isolated from coastal sediment, and transfer of Planococcus psychrophilus and Planococcus alkanoclasticus to Planomicrobium as Planomicrobium psychrophilum comb. nov. and Planomicrobium alkanoclasticum comb. nov, Int. J. Syst. Evol. Microbiol., 55, 699–702, 2005. Dancer, S. J., Shears, P., and Platt, D. J.: Isolation and characterization of coliforms from glacial ice and water in Canada's High Arctic, J. Appl. Microbiol., 82, 597–609, 1997. Green, P. N.: The genus Methylobacterium: The Prokaryotes, 2nd ed., edited by: Balows, A., Truper, H. G., Dworkin, M., Harder, W., and Schleifer, K.-H., 2342–2349, New York, Springer-Verlag Holland, MA, 1992. Groudieva, T., Kambourova, M., Yusef, H., Royter, M., Grote, R., Trinks, H., and Antranikian, G.: Diversity and cold-active hydrolytic enzymes of culturable bacteria associated with Arctic sea ice, Spitzbergen, Extremophile, 8, 475–488, 2004. Li, G.: Dynamic Meteorological of the Tibetan Plateau, China Meteorological Press, Beijing, 2002 (In Chinese). Kang, S., Wake, C. P., Qin, D., Mayewski, P. A., and Yao, T.: Monsoon and dust signals recorded in the Dasuopu firn core, central Himalayas, J. Glaciol., 46, 222–226, 2000. Kang, S. C., Mayewski, P. A., Qin, D. H., Yan, Y. P., Hou, S. G., Zhang, D. Q., Ren, J. W., and Kreutz, K. J.: Glaciochemical records from a Mt. Everest ice core: relationship to atmospheric circulation over Asia, Atmos. Environ., 36, 3351–3361, 2002. Kumar, S. and Tamura, K. M. N.: MEGA molecular evolutionary genetics analysis, version 1.01. Pennsylvania State University, University Park, 1993. Marinoni, A., Polesello, S., Smiraglia, C., and Valsecchi, S.: Chemical composition of freshsnow samples from the southern slope of Mt. Everest region (Khumbu–Himal region, Nepal), Atmos. Environ., 35, 3183–3190, 2001. Priscu, J. C., Fritsen, C. H., Adams, E. E., Giovannoni, S. J., Paerl, H. W., McKay, C. P., Doran, P. T., Gordon, D. A., Lanoil, B. D., and Pinckney J. L. Perennial Antarctic lake ice: an oasis for life in a polar desert, Science, 280, 2095–2098, 1998. Priscu, J. C., Adams, E. E., Lyons, W. B., Voytek, M. A., Mogk, D. W., Brown, R. L., McKay, C. P., Takacs, C. D., Welch, K. A., Wolf, C. F., Kirshtein, J. D., and Avci, R.: Geomicrobiology of subglacial ice above Lake Vostok, Antarctica, Science, 286, 2141–2144, 1999. Sander, M. and Schmieger, H.: Method for host-independent detection of generalized transducing bacteriophages in natural habitats, Appl. Environ. Microbiol., 67, 1490–1493, 2001. Takami, H., Inoue, A., Fuji, F., and Horikoshi, K.: Microbial flora in the deepest sea mud of the Mariana Trench, FEMS Microbiol. Lett., 152, 279–285, 1997. Tang, M.: Formation, evolution and variability characteristics of Qinghai-Tibetan Plateau Monsoon. In: Contemporary climatic variations over Qinghai-Tibetan Plateau and their influences on environments (ed. Tang, M., Chen, G., Lin, Z.), Guangdong Sciences and Technology Press, Guangzhou, 161–182, 1998. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F., and Higgins, D. G.: The CLUSTAL-X Windows interface-flexible strategies for multiple sequence alignment aided by quality analysis tools, Nucleic Acids Res., 24, 4876–4882, 1997. Thompson, L. G., Yao, T. D., Mosley-Thompson, E., Davis, M. E., Henderson, K. A., and Lin, P.-N.: A high-resolution millennial record of the South Asian Monsoon from Himalayan ice cores, Science, 289, 1916–1919, 2000. Tiago, I., Chung, A. P., and Ver\'issimo, A.: Bacterial diversity in a nonsaline alkaline environment: heterotrophic aerobic populations, Appl. Environ. Microbiol., 70, 7378–7387, 2004. Tian, L., Masson-Delmotte, V., Stievenard, M., Yao, T., and Jouzel, J.: Tibetan plateau summer monsoon northward extent revealed by measurements of water stable isotopes, J. Geophys. Res., 106(D22), 28 081–28 088, 2001. Tian, L., Yao, T., Schuster, P. F., White, J. W. C., Ichiyanagi, K., Pendall, E., Pu, J., and Yu, W.: Oxygen-18 concentrations in recent precipitation and ice cores on the Tibetan Plateau, J. Geophys. Res., 108(D9), 4293–4302, 2003. Wake, C. P., Dibb, J. E., Mayewski, P. A., Li, Z. Q., and Xie, Z. C.: The chemical composition of aerosols over the eastern Himalayas and Tibetan Plateau during low dust periods, Atmos. Environ., 28, 695–704, 1994. Willerslev, E., Hansen, A. J., Christensen, B., Steffensen, J. P., and Arctander, P.: Diversity of Holocene life forms in fossil glacier ice, Proc. Natl. Acad. Sci., 96, 8017–8021, 1999. Wu, G., Yao, T., Thompson, L. G., and Li, Z.: Microparticle record in the Guliya ice core and its comparison with polar records since the last interglacial, Chin. Sci. Bull., 49(6), 607–611, 2004. Wu, G. and Zhang, Y.: Tibetan Plateau forcing and the monsoon onset over South Asia and the South China Sea, Mon. Wea. Rev., 126, 913–927, 1998. Xiang, S. R., Yao, T. D., An, L. Z., Xu, B. Q., and Wang, J. X.: 16S rRNA Sequences and Differences in Bacteria Isolated from the Muztag Ata Glacier at Increasing Depths, Appl. Environ. Microbiol., 71, 4619–4627, 2005a. Xiang, S. R., Yao, T. D., An, L. Z., Wu, G. J., Xu, B. Q., Ma X. J., Li, Z., Wang J. X., and Yu, W. S.: Vertical quantitative and dominant population distribution of the bacteria in the Muztagh Ata ice core, Sci. China, D48(10), 1728–1739, 2005b. Xiao, C. D., Kang, S. C., Qin, D. H., Yao, T. D., and Ren, J. W.: Transport of atmospheric impurities over the Qinghai-Xizang (Tibetan) Plateau as shown by snow chemistry, J. Asian Earth Sci., 20, 231–239, 2002. Yao, T., Wu, G., Pu, J., Jiao, K., and Huang, C.: Relationship between calcium and atmospheric dust recorded in Guliya ice core, Chin. Sci. Bull., 49, 706–710, 2004. Yao, T., Xiang, S., Zhang, X., Wang, N., and Wang, Y.: Microorganisms in the Malan ice core and their relation to climatic and environmental changes, Global Biogeochem. Cycles, 20, GB1004, doi:10.1029/2004GB002424, 2006. Zhang, D. Q., Qin, D. H., Hou, S. G., Kang, S. C., Ren, J. W., and Mayewski. P. A.: Climatic significance of record from an 80.36 m ice core in the East Rongbuk Glacier, Mount Qomolangma (Everest), Sci. China, D48(2), 266–272, 2005. Zhang, X. J., Yao, T. D., Ma, X. J., and Wang, N. L.: Analysis of the characteristics packed in the ice core of Malan Glacier, Tibet, China, Sci. China, D44 (suppl.), 369–374, 2001. Zhang, X. J., Ma, X., Yao, T., and Zhang, G.: Diversity of 16S rRNA and environmental factor influencing bacteria in Malan ice core, Chin. Sci. Bull., 48 (11), 1146–1150, 2003. Zhou, J. Z., Bruns, M. A., and Tiedje, J. M.: DNA recovery from soils of diverse composition, Appl. Environ. Microbiol., 62, 316–322, 1996. Zhou, S. Meteorology and Climatology (3rd edit). High Education Press, Beijing. 1997 (In Chinese).