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The nonenzymatic template-directed ligation of oligonucleotides
Institute of Chemical Biology and Fundamental Medicine SB RAS, Lavrentiev Avenue 8, Novosibirsk 630090, Russia
Abstract. The nonenzymatic template-directed ligation of oligonucleotides containing 2', 3'-cyclic phosphate was investigated in the presence of divalent cations. Ligation of the oligonucleotides readily occurred in the presence of Mg2+, Mn2+, Co2+, Zn2+, Pb2+. Efficacy of the metal ion catalysts inversely correlated with pKa values of the metal-bound water molecules. The intermolecular transesterification reaction yielded at least 95metal ion. Relatively high reaction yields (up to 15fragmentation to oligonucleotides with 2',3'-cyclic phosphates, followed by reactions of those oligonucleotides could provide a source of new RNA molecules under prebiotic conditions.
Final Revised Paper (PDF, 431 KB) Discussion Paper (BGD)
Citation: Lutay, A. V., Chernolovskaya, E. L., Zenkova, M. A., and Vlassov, V. V.: The nonenzymatic template-directed ligation of oligonucleotides, Biogeosciences, 3, 243-249, 2006. Bibtex EndNote Reference Manager
