1Institute of Desertification Studies, Chinese Academy of Forestry,
2Research Institute of Forestry, Chinese Academy of Forestry, Beijing,
3Environmental Sciences Division and Climate Change Science Institute,
Oak Ridge National Laboratory, Oak Ridge, TN 37831, USA
4The Experimental Center of Desert Forestry of the Chinese Academy of
Forestry, Dengkou, Inner Mongolia Autonomous Region, China
5Center for Earth System Science, Tsinghua University, Beijing, China
6Headquarters, Chinese Academy of Forestry, Beijing, China
Received: 28 May 2016 – Discussion started: 06 Jun 2016
Abstract. Understanding intra-plant variations in δ15N is essential for fully utilizing the potential of δ15N as an integrator of the terrestrial nitrogen (N) cycle and as an indicator of the relative limitation of N and phosphorous (P) on plant growth. Studying such variations can also yield insights into N metabolism by plant as a whole or by specific organs. However, few researchers have systematically evaluated intra-plant variations in δ15N and their relationships with organ nutrient contents. We excavated whole plant architectures of Nitraria tangutorum Bobrov, a C3 species of vital regional ecological importance, in two deserts in northwestern China. We systematically and simultaneously measured N isotope ratios and N and P contents of different parts of the excavated plants. We found that intra-plant variations in δ15N of N. tangutorum were positively correlated with corresponding organ N and P contents. However, it was the N × P interaction, not N and P individually or their linear combination, that was the strongest predictor of intra-plant δ15N. Additionally, we showed that root δ15N increased with depth into soil, a pattern similar to profiles of soil δ15N reported by previous studies in different ecosystems. We hypothesized that the strong positive intra-plant δ15N–N and P relationships are caused by three processes acting in conjunction: (1) N and P content-driven fractionating exchanges of ammonia between leaves and the atmosphere (volatilization) during photorespiration, (2) resorption and remobilization of N and P from senescing leaves, and (3) mixture of the re-translocated foliar N and P with existing pools in stems and roots. To test our hypothesis, future studies should investigate plant N volatilization and associated isotope fractionation and intra-plant variations in δ15N in different species across ecosystems and climates.
Revised: 24 Nov 2016 – Accepted: 10 Dec 2016 – Published: 11 Jan 2017
Zhang, J., Gu, L., Zhang, J., Wu, R., Wang, F., Lin, G., Wu, B., Lu, Q., and Meng, P.: The interaction between nitrogen and phosphorous is a strong predictor of intra-plant variation in nitrogen isotope composition in a desert species, Biogeosciences, 14, 131-144, doi:10.5194/bg-14-131-2017, 2017.