Journal cover Journal topic
Biogeosciences An interactive open-access journal of the European Geosciences Union
Biogeosciences, 14, 1021-1038, 2017
© Author(s) 2017. This work is distributed
under the Creative Commons Attribution 3.0 License.
Research article
03 Mar 2017
Quantification of multiple simultaneously occurring nitrogen flows in the euphotic ocean
Min Nina Xu1, Yanhua Wu2, Li Wei Zheng1, Zhenzhen Zheng1, Huade Zhao3, Edward A. Laws4, and Shuh-Ji Kao1 1State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen, China
2Shenzhen Marine Environment Monitoring Center Station, State Oceanic Administration, Shenzhen, China
3National Marine Environmental Monitoring Center, Dalian, China
4Environmental Sciences Department, School of the Coast & Environment, Louisiana State University, Baton Rouge, USA
Abstract. The general features of the N cycle in the sunlit region of the ocean are well known, but methodological difficulties have previously confounded simultaneous quantification of transformation rates among the many different forms of N, e.g., ammonium (NH4+), nitrite (NO2), nitrate (NO3), and particulate/dissolved organic nitrogen (PN/DON). However, recent advances in analytical methodology have made it possible to employ a convenient isotope labeling technique to quantify in situ fluxes among oft-measured nitrogen species within the euphotic zone. Addition of a single 15N-labeled NH4+ tracer and monitoring of the changes in the concentrations and isotopic compositions of the total dissolved nitrogen (TDN), PN, NH4+, NO2, and NO3 pools allowed us to quantify the 15N and 14N fluxes simultaneously. Constraints expressing the balance of 15N and 14N fluxes between the different N pools were expressed in the form of simultaneous equations, the unique solution of which via matrix inversion yielded the relevant N fluxes, including rates of NH4+, NO2, and NO3 uptake; ammonia oxidation; nitrite oxidation; DON release; and NH4+ uptake by bacteria. The matrix inversion methodology that we used was designed specifically to analyze the results of incubations under simulated in situ conditions in the euphotic zone. By taking into consideration simultaneous fluxes among multiple N pools, we minimized potential artifacts caused by non-targeted processes in traditional source–product methods. The proposed isotope matrix method facilitates post hoc analysis of data from on-deck incubation experiments and can be used to probe effects of environmental factors (e.g., pH, temperature, and light) on multiple processes under controlled conditions.

Citation: Xu, M. N., Wu, Y., Zheng, L. W., Zheng, Z., Zhao, H., Laws, E. A., and Kao, S.-J.: Quantification of multiple simultaneously occurring nitrogen flows in the euphotic ocean, Biogeosciences, 14, 1021-1038, doi:10.5194/bg-14-1021-2017, 2017.
Publications Copernicus
Short summary
To resolve multiple N transformation rates, we proposed an innovative “isotope matrix method” to simultaneously derive rates for multiple transformations. This method was designed specifically for incubations in the euphotic zone under simulated in situ light conditions and minimized potential biases caused by non-targeted processes. The method facilitates simple post hoc analysis of data and can be used to probe specific effects of environmental factors on the rates of interactive N processes.
To resolve multiple N transformation rates, we proposed an innovative “isotope matrix...