Abstract. As the field of ocean acidification has grown, researchers have increasingly turned to laboratory experiments to understand the impacts of increased CO₂ on marine organisms. However, other changes such as ocean warming and deoxygenation are occurring concurrently with the increasing CO₂ concentrations, complicating the understanding of the impacts of anthropogenic changes on organisms. This experimental aquarium design allows for independent regulation of CO₂ concentration, O₂ levels, and temperature in a controlled environment to study the impacts of multiple stressors. The system has the flexibility for a wide range of treatment chemistry, seawater volumes, and study organisms. Control of the seawater chemistry is achieved by equilibration of a chosen gas mixture with seawater using a Liqui-Cel^® membrane contactor. Included as examples, two experiments performed using the system have shown control of CO₂ at values between approximately 500 and 1400 μatm and O₂ at values from 80 to 240 μmol kg⁻¹. Temperature has been maintained to 0.5 °C or better in the range of 10–17 °C. On a weeklong timescale, the system has achieved variability in pH of less than 0.007 pH units and in oxygen concentration of less than 3.5 μmol kg⁻¹. Longer experiments, over a month in duration, have been completed with control to better than 0.08 pH units and 13 μmol kg⁻¹ O₂. The ability to study the impacts of multiple stressors in the laboratory simultaneously, as well as independently, will be an important part of understanding the response of marine organisms to a high-CO₂ world.